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    Method For the Fermentative Production of L-Valine and Suitable Microorganism
    11.
    发明申请
    Method For the Fermentative Production of L-Valine and Suitable Microorganism 失效
    L-缬氨酸和合适的微生物发酵生产方法

    公开(公告)号：US20080153139A1

    公开(公告)日：2008-06-26

    申请号：US11663984

    申请日：2006-04-06

    申请人： Jan Marienhagen ,  Lothar Eggeling ,  Hermann Sahm

    发明人： Jan Marienhagen ,  Lothar Eggeling ,  Hermann Sahm

    IPC分类号： C12P13/08 ,  C12N1/21

    CPC分类号： C12P13/08 ,  C12N9/1096

    摘要： The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacteriuim glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.

    摘要翻译： 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌（Corynebacteriuim glutamicum）的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。

    Production of L-isoleucine by means of recombinant microorganisms with deregulated threonine dehydratase
    14.
    发明授权
    Production of L-isoleucine by means of recombinant microorganisms with deregulated threonine dehydratase 失效
    通过具有失调的苏氨酸脱水酶的重组微生物生产L-异亮氨酸

    公开(公告)号：US6107063A

    公开(公告)日：2000-08-22

    申请号：US669378

    申请日：1997-03-20

    申请人： Bettina Moeckel ,  Lothar Eggeling ,  Hermann Sahm

    发明人： Bettina Moeckel ,  Lothar Eggeling ,  Hermann Sahm

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/88 ,  C12N15/00 ,  C12N15/60 ,  C12P13/06 ,  C12R1/15 ,  C12N15/31 ,  C12N15/63

    CPC分类号： C12N9/88 ,  C12P13/06

    摘要： The invention relates to processes for the microbial production of L-isoleucine. To this end, in a gene in vitro of a threonine dehydratse, one or more bases in the gene region coding the enzyme's allosteric domains are exchanged in such a way that at least one amino acid in the amino acid sequence of the allosteric domains of the enzyme is replaced by another so that the enzyme is no longer inhibited by L-isoleucine feedback. Furthermore, concrete amino acid exchanges in the amino acid sequence of the enzyme are effected in a gene in vitro of a threonin dehydratase of Corynebacterium glutamicum by base exchange both outside and inside and outside the gene region coding the allosteric domains of the enzyme si that, after the transformation of such mutated threonine dehydratase genes into a threonine or L-isoleucine-producing host cell, the latter repeatedly forms L-isoleucine.

    摘要翻译： PCT No.PCT / DE95 / 00017 Sec。 371日期1997年3月20日 102（e）1997年3月20日PCT PCT 1995年1月9日PCT公布。 WO95 / 19442 PCT公开号 日期1995年7月20日本发明涉及L-异亮氨酸的微生物生产方法。 为此，在苏氨酸脱水体外的基因的基因中，编码酶变构域的基因区域中的一个或多个碱基以这样的方式交换，使得至少一个氨基酸序列的变构域 酶被另一个替代，使得酶不再被L-异亮氨酸反馈抑制。 此外，酶的氨基酸序列中的具体氨基酸交换通过在编码酶si的变构结构域的基因区域的外部和外部的碱基交换体外在谷氨酸棒杆菌的苏氨酸脱水酶的基因中进行， 在将这种突变的苏氨​​酸脱水酶基因转化成产生苏氨酸或L-异亮氨酸的宿主细胞后，后者重复形成L-异亮氨酸。

    Process for the microbial production of amino acids by boosted activity of export carriers
    16.
    发明授权
    Process for the microbial production of amino acids by boosted activity of export carriers 失效
    通过出口承运人的加强活动，微生物生产氨基酸的过程

    公开(公告)号：US06858406B1

    公开(公告)日：2005-02-22

    申请号：US09105117

    申请日：1998-06-17

    申请人： Marina Vrlijc ,  Lothar Eggeling ,  Hermann Sahm

    发明人： Marina Vrlijc ,  Lothar Eggeling ,  Hermann Sahm

    IPC分类号： C12N15/09 ,  C07K14/34 ,  C12N1/21 ,  C12N15/00 ,  C12N15/31 ,  C12N15/63 ,  C12N15/77 ,  C12P13/04 ,  C12P13/08 ,  C12P21/02 ,  C12R1/15 ,  C12P21/06 ,  C07H21/04 ,  C07K14/00 ,  C12N1/20 ,  C12N5/00

    CPC分类号： C07K14/34 ,  C12N15/77 ,  C12P13/04 ,  C12P13/08

    摘要： The invention pertains to a process for the microbial production of amino acids. The process involves boosting the export carrier activity and/or export gene expression of a microorganism which produces the desired amino acid. It was found that a single specific gene is responsible for the export of a given amino acid, and on that basis, a process for the microbial production of amino acids, involving the controlled boosting of the export gene expression and/or export carrier activity of a microorganism was developed, which produces the amino acid. The boosted expression or activity of the export carrier resulting from this process increases the secretion rate and thus increases transport of the desired amino acid.

    摘要翻译： 本发明涉及一种微生物生产氨基酸的方法。 该方法包括增加产生所需氨基酸的微生物的出口载体活性和/或导出基因表达。 已经发现，单个特定基因负责输出给定的氨基酸，并且在此基础上，氨基酸的微生物生产过程涉及出口基因表达的受控增强和/或导出载体活性 开发出产生氨基酸的微生物。 由该方法产生的出口载体的增强的表达或活性增加分泌速率，从而增加所需氨基酸的转运。

    Method for microbially producing L-valine
    20.
    发明授权
    Method for microbially producing L-valine 失效
    微生物生产L-缬氨酸的方法

    公开(公告)号：US07632663B1

    公开(公告)日：2009-12-15

    申请号：US09914006

    申请日：2000-02-21

    申请人： Lothar Eggeling ,  Hermann Sahm

    发明人： Lothar Eggeling ,  Hermann Sahm

    IPC分类号： C12P13/04 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/00

    CPC分类号： C12N9/93 ,  C12N9/1014 ,  C12N9/88 ,  C12P13/06

    摘要： The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.

    摘要翻译： 本发明涉及微生物生产L-缬氨酸的方法，其中二羟基酸合酶（ilvD）活性和/或ilvD基因表达在微生物中增强。 作为替代或与其结合，在微生物中增强乙酰羟酸合酶（ilvBN）活性和异构还原酶（ilvC）活性和/或ilvBNC基因表达。 根据本发明的方法优选利用其中减少或消除了减少L-缬氨酸形成的参与代谢途径的至少一种酶的活性的微生物。 因此，例如，根据本发明的方法优选利用在苏氨酸脱水酶（ilvA）基因中具有缺陷突变的微生物和/或泛酸合成的一种或多种基因中的缺陷突变。