公开(公告)号：US20060263801A1

公开(公告)日：2006-11-23

申请号：US11361312

申请日：2006-02-24

Applicant: James Marks ,  Ulrik Nielsen ,  Dimitri Kirpotin

Inventor： James Marks ,  Ulrik Nielsen ,  Dimitri Kirpotin

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: G01N33/5011 ,  C40B30/04 ,  G01N33/5008 ,  G01N33/5432 ,  G01N33/566 ,  G01N33/6845 ,  G01N33/6854 ,  G01N33/6863

Abstract: This invention provides methods of identifying ligands that are internalized into a cell. The methods typically involve i) contacting the cell with a reporter non-covalently coupled to a ligand; ii) dissociating the reporter from the ligand and removing dissociated reporter from the surface of the cell; and iii) detecting the reporter within said cell (if any is present) where the presence of the reporter within said cell indicates that the ligand binds to an internalizing receptor and is internalized.

公开(公告)号：US20060099205A1

公开(公告)日：2006-05-11

申请号：US11154103

申请日：2005-06-15

Applicant: Gregory Adams ,  Eva Horak ,  Louis Weiner ,  James Marks

Inventor： Gregory Adams ,  Eva Horak ,  Louis Weiner ,  James Marks

IPC: A61K39/395 ,  C07K16/44 ,  C07K16/28

CPC classification number: A61K39/395 ,  A61K51/109 ,  A61K2039/505 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/622 ,  G01N2333/71

Abstract: Bispecific single chain antibody molecules are disclosed which may be used to advantage to treat various forms of cancer associated with the overexpression of members of the EGFR protein family.

Abstract translation: 公开了双特异性单链抗体分子，其可用于治疗与EGFR蛋白家族的成员的过表达相关的各种形式的癌症。

公开(公告)号：US06868736B2

公开(公告)日：2005-03-22

申请号：US10371871

申请日：2003-02-21

Applicant: Takeo Sawatari ,  Alex Klooster ,  Dean M. Aslam ,  Yuping Lin ,  James Marks

Inventor： Takeo Sawatari ,  Alex Klooster ,  Dean M. Aslam ,  Yuping Lin ,  James Marks

IPC: G01L7/08 ,  G01L9/00 ,  G02B6/32 ,  G02B6/38 ,  G01L1/24 ,  F16C11/00

CPC classification number: G02B6/3869 ,  G01L9/0042 ,  G01L9/0047 ,  G01L9/0077 ,  G02B6/32 ,  G02B6/3855

Abstract: The fiber optic pressure sensing system includes a sensor housing formed using MEMS processing. The sensor housing has ribs and grooves in both horizontal and vertical directions relative to the surface to allow the membrane to flex in a consistent manner. The flexing of the membrane allows the pedestal to be repeatedly positioned in response to pressure acting on the extension of the sensor head and membrane.

Abstract translation: 光纤压力传感系统包括使用MEMS处理形成的传感器外壳。 传感器外壳在水平和垂直方向上具有相对于表面的肋和槽，以允许膜以一致的方式弯曲。 膜的弯曲允许基座响应于作用在传感器头和膜的延伸部上的压力而重复定位。

公开(公告)号：US07350311B2

公开(公告)日：2008-04-01

申请号：US11398781

申请日：2006-04-05

Applicant: James Marks

Inventor： James Marks

IPC: B43L9/00 ,  B23K37/00

CPC classification number: B43L13/20

Abstract: A universal reusable template for cutting ends of cylindrical objects such as pipes, in preparation for joining the cylindrical objects to other cylindrical bodies. The template is formed as a flat flexible bendable sheet with magnetic properties allowing the sheet to be temporarily secured on a metal pipe. The front face of the sheet has reference indicia corresponding to contour lines of a desired cut to be made in the end of the cylindrical body. The cut lines represent most conventional types and sizes of lateral-type cuts, eccentric cuts and saddle-type cuts, with pipe diameters ranging from about 3″ to about 24″.

Abstract translation: 用于切割诸如管子的圆柱形物体的端部的通用可重用模板，以准备将圆柱形物体接合到其它圆柱体。 模板形成为具有磁性能的扁平柔性可弯曲片材，其允许片材临时固定在金属管上。 片材的正面具有对应于要在圆柱体的端部制成的期望切口的轮廓线的参考标记。 切割线表示大多数常规类型和尺寸的横向切割，偏心切割和鞍形切割，管直径范围为约3“至约24”。

公开(公告)号：US20080050359A1

公开(公告)日：2008-02-28

申请号：US11625750

申请日：2007-01-22

Applicant: Andrew Griffiths ,  Hendricus Renerus Jacobus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

Inventor： Andrew Griffiths ,  Hendricus Renerus Jacobus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

IPC: A61K31/395 ,  A61P43/00 ,  C07K16/00 ,  C40B20/08

CPC classification number: C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

Abstract translation: 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所用的核酸文库可能来源于未免疫哺乳动物的重排的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US20070232790A1

公开(公告)日：2007-10-04

申请号：US11625769

申请日：2007-01-22

Applicant: ANDREW GRIFFITHS ,  Hendricus Renerus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

Inventor： ANDREW GRIFFITHS ,  Hendricus Renerus Hoogenboom ,  James Marks ,  John McCafferty ,  Gregory Winter ,  Geoffrey Grigg

IPC: C07K16/18

CPC classification number: C40B40/02 ,  B01J2219/00718 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2812 ,  C07K16/2863 ,  C07K16/2866 ,  C07K16/3007 ,  C07K16/3092 ,  C07K16/34 ,  C07K16/40 ,  C07K16/4208 ,  C07K16/4241 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  Y10S530/867

Abstract: Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.

Abstract translation: 公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的特定哺乳动物种类的抗体或片段。 方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其作为抗体或抗体片段的特异性结合对的表面成员显示，并且每个含有rgdp的核酸序列衍生自物种 的哺乳动物。 每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。 通过与来自所述哺乳动物物种的自身抗原结合来选择抗自身抗体片段。 显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。 所用的核酸文库可能来源于未免疫哺乳动物的重排的V基因序列。 描述和显示合成或人造文库是有用的。

公开(公告)号：US20060287509A1

公开(公告)日：2006-12-21

申请号：US10092640

申请日：2002-03-05

Applicant: James Marks ,  Robert Schier

Inventor： James Marks ,  Robert Schier

IPC: C07K16/28

CPC classification number: C07K16/32 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/77 ,  C07K2317/92 ,  C07K2319/00 ,  F16H59/105 ,  F16H61/32 ,  F16H2061/326 ,  G01N33/57492 ,  G01N2333/71

Abstract: This invention provides for novel human antibodies that specifically bind to c-erbB-2. The antibodies may be used alone or as components of chimeric molecules that specifically target and deliver effector molecules to cells overexpressing c-erbB-2.

Abstract translation: 本发明提供特异性结合c-erbB-2的新型人抗体。 抗体可以单独使用或作为特异性靶向并将效应分子递送至过量表达c-erbB-2的细胞的嵌合分子的组分使用。

公开(公告)号：US20060115808A1

公开(公告)日：2006-06-01

申请号：US11238245

申请日：2005-09-29

Applicant: Michael Cardone ,  Ulrik Nielsen ,  Gavin MacBeath ,  James Marks ,  Peter Sorger ,  Anthony Sinskey

Inventor： Michael Cardone ,  Ulrik Nielsen ,  Gavin MacBeath ,  James Marks ,  Peter Sorger ,  Anthony Sinskey

IPC: C12Q1/70 ,  G01N33/542 ,  C12M1/34 ,  G01N33/53

CPC classification number: C40B30/04 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00725 ,  C07K17/06 ,  C40B40/10 ,  G01N33/5079 ,  G01N33/54353 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6845 ,  G01N33/6848 ,  G01N33/6851

Abstract: Disclosed are products and methods to facilitate the identification of compounds that are capable of interacting with biological macromolecules of interest, especially when such macromolecules are attached to a support surface in microarray. Aspects of the invention concern attachment chemistry, peptide labeling, antibody preparation, applications and so on.