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8 条记录 (耗时 0.033 秒)

    POLYHYDROXYBUTYRATE POLYMERASE
    4.
    发明申请
    POLYHYDROXYBUTYRATE POLYMERASE 失效
    聚羟基丁酸酯聚合物

    公开(公告)号：US20050064565A1

    公开(公告)日：2005-03-24

    申请号：US10341214

    申请日：2003-01-13

    申请人： Oliver Peoples ,  Anthony Sinskey

    发明人： Oliver Peoples ,  Anthony Sinskey

    IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N15/52 ,  C12N15/60 ,  C12P7/62

    CPC分类号： C12N9/0006 ,  C12N9/00 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/625

    摘要： A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.

    摘要翻译： 通过在原核和真核细胞，特别是植物中的分子水平处理聚羟基丁酸酯（PHB）和聚羟基链烷酸酯（PHA）聚酯的遗传学和酶学方法来控制和改变生物聚合物合成的方法。 实施例证明参与PHB和PHA聚合物生产的基因的分离，表征和表达。 鉴定或分离来自Zoogloea苎麻菌株I-16-M，产碱假单胞菌，痢疾杆菌（Nocardia salmonicolur）和橄榄油（Psuedomonas olevarans）的PHB和PHA合成途径（β-酮硫解酶，乙酰乙酰辅酶A还原酶和PHB聚合酶或PHA聚合酶）中的酶编码基因 并在非PHB生物体大肠杆菌中表达。 对聚合物的具体修饰包括聚合物链长度的变化以及将不同单体引入到聚合物中以产生具有不同物理性质的共聚物。

    Polyhydroxybutyrate polymerase
    5.
    发明申请
    Polyhydroxybutyrate polymerase 失效

    公开(公告)号：US20060040281A1

    公开(公告)日：2006-02-23

    申请号：US11086605

    申请日：2005-03-22

    申请人： Oliver Peoples ,  Anthony Sinskey

    发明人： Oliver Peoples ,  Anthony Sinskey

    IPC分类号： C12Q1/68 ,  C12P7/62 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12N9/00 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/625

    摘要： A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerase or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and Psuedomonas olevarans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.

    Microbioreactor for continuous cell culture
    6.
    发明申请
    Microbioreactor for continuous cell culture 审中-公开
    用于连续细胞培养的微生物反应器

    公开(公告)号：US20060199260A1

    公开(公告)日：2006-09-07

    申请号：US11236453

    申请日：2005-09-26

    申请人： Zhiyu Zhang ,  Paolo Boccazzi ,  Hyun-Goo Choi ,  Klavs Jensen ,  Anthony Sinskey

    发明人： Zhiyu Zhang ,  Paolo Boccazzi ,  Hyun-Goo Choi ,  Klavs Jensen ,  Anthony Sinskey

    IPC分类号： C12M3/00 ,  C12M1/12

    CPC分类号： C12M23/16 ,  B01F13/0059 ,  B01F13/0827 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502723 ,  B01L7/00 ,  B01L2300/0822 ,  B01L2300/0887 ,  B01L2300/10 ,  B82Y30/00 ,  C12M23/24 ,  C12M23/34 ,  C12M27/02 ,  C12M29/04 ,  C12M41/26 ,  C12M41/32

    摘要： The present invention microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes of less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. Methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The microbioreactors having a variety of different designs, some of which incorporate active fluid mixing and/or have the capability to operate in batch, fed-batch, or continuous mode. In certain embodiments the microreactors operate as microchemostats. Methods for culturing cells under chemostat conditions in a microbioreactor are also provided.

    摘要翻译： 本发明的用于培养细胞的微生物生物反应器（微发酵器）和微生物生物反应器阵列。 微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长（例如细菌细胞生长）的浓度向容器内部提供氧气的装置。 取决于实施方案，微发酵罐容器可以具有小于约1ml的各种内部体积。 微发酵器可以包括曝气膜和任选的各种感测装置。 提供了使用微发酵器的方法，例如选择最佳细胞株或生物过程参数。 具有各种不同设计的微生物反应器，其中一些结合了活性流体混合和/或具有以批量，补料分批或连续模式操作的能力。 在某些实施方案中，微反应器作为微量化学剂操作。 还提供了在微生物反应器中在恒化器条件下培养细胞的方法。

    Feedback-resistant pyruvate carboxylase gene from corynebacterium
    8.
    发明申请
    Feedback-resistant pyruvate carboxylase gene from corynebacterium 有权
    来自棒状杆菌的反馈抗性丙酮酸羧化酶基因

    公开(公告)号：US20050221452A1

    公开(公告)日：2005-10-06

    申请号：US11136887

    申请日：2005-05-25

    申请人： Paul Hanke ,  Anthony Sinskey ,  Laura Willis ,  Stephane Guillouet

    发明人： Paul Hanke ,  Anthony Sinskey ,  Laura Willis ,  Stephane Guillouet

    IPC分类号： C12N1/21 ,  C12N9/00 ,  C12P13/08 ,  C07H21/04 ,  C12N9/88 ,  C12P13/20

    CPC分类号： C12N9/93 ,  C12P13/08 ,  C12Y604/01001

    摘要： The present invention relates to a mutated pyruvate carboxylase gene from Corynebacterium. The mutant pyruvate carboxylase gene encodes a pyruvate carboxylase enzyme which is resistant to feedback inhibition from aspartic acid. The present invention also relates to a method of replacing the wild-type pyruvate carboxylase gene in Corynebacterium with this feedback-resistant pyruvate carboxylase gene. The present invention further relates to methods of the production of amino acids, preferably lysine, comprising the use of this mutant pyruvate carboxylase enzyme in microorganisms.

    摘要翻译： 本发明涉及来自棒状杆菌的突变型丙酮酸羧化酶基因。 突变体丙酮酸羧化酶基因编码丙酮酸羧化酶，其对天冬氨酸的反馈抑制具有抗性。 本发明还涉及用该反馈抗性丙酮酸羧化酶基因替代棒状杆菌中的野生型丙酮酸羧化酶基因的方法。 本发明还涉及生产氨基酸，优选赖氨酸的方法，其包括在微生物中使用该突变型丙酮酸羧化酶。