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    Method for producing methacrylic acid acrylic acid with a combination of enzyme catalysts
    14.
    发明授权
    Method for producing methacrylic acid acrylic acid with a combination of enzyme catalysts 失效
    用酶催化剂组合生产甲基丙烯酸和丙烯酸的方法

    公开(公告)号：US06670158B2

    公开(公告)日：2003-12-30

    申请号：US10067652

    申请日：2002-02-05

    申请人： Robert Dicosimo ,  Robert D. Fallon ,  John E. Gavagan ,  Leo Ernest Manzer

    发明人： Robert Dicosimo ,  Robert D. Fallon ,  John E. Gavagan ,  Leo Ernest Manzer

    IPC分类号： C12P1300

    CPC分类号： C12P7/40

    摘要： The invention provides a process for the hydrolysis of acrylonitrile to acrylic acid, and for the hydrolysis of methacrylonitrile to methacrylic acid, in high yield and at high concentration with high specificity. Acrylonitrile or methacrylonitrile is hydrolyzed in a suitable aqueous reaction mixture by a catalyst characterized by a nitrile hydratase and amidase activity of Comamonas testosteroni 5-MGAM-4D, producing the corresponding acid. The acrylic acid or methacrylic acid is isolated as the acid or corresponding salt.

    摘要翻译： 本发明提供了一种将丙烯腈水解成丙烯酸，并以高产率和高浓度高特异性水解甲基丙烯腈至甲基丙烯酸的方法。 丙烯腈或甲基丙烯腈在合适的水性反应混合物中通过催化剂水解，其特征在于产生相应酸的腈水合酶和酰胺酶活性的Comamonas testosteroni 5-MGAM-4D。 分离丙烯酸或甲基丙烯酸作为酸或相应的盐。

    Producing diastereomers of 4-hydroxyproline using 4-hp epimerase from Serratia marcescens or Acinetobacter baumanni
    15.
    发明授权
    Producing diastereomers of 4-hydroxyproline using 4-hp epimerase from Serratia marcescens or Acinetobacter baumanni 失效
    使用来自粘质沙雷氏菌或鲍氏不动杆菌的4-hp差向异构酶生产4-羟脯氨酸的非对映异构体

    公开(公告)号：US06204050B1

    公开(公告)日：2001-03-20

    申请号：US09356838

    申请日：1999-07-19

    申请人： Robert Dicosimo ,  Susan K. Fager ,  John E. Gavagan

    发明人： Robert Dicosimo ,  Susan K. Fager ,  John E. Gavagan

    IPC分类号： C12P4100

    CPC分类号： C12R1/43 ,  C12N9/90 ,  C12P13/24 ,  C12R1/01

    摘要： The present invention relates to two novel microorganisms, Acinetobacter baumanni WT-50/50-4A (ATCC 202144) and Serratia marcescens WT-L-4A1(RED) (ATCC 202145) characterized by 4-hydroxyproline epimerase activity. The compounds trans-4-hydroxy-L-proline (THLP) or cis-4-hydroxy-D-proline (CHDP) are converted with this epimerase activity to a mixture of THLP and CHDP. Additionally, the compounds trans-4-hydroxy-D-proline (THDP) or cis-4-hydroxy-L-proline (CHLP) are converted with this epimerase activity to a mixture of THDP and CHLP.

    摘要翻译： 本发明涉及以4-羟基脯氨酸差向异构酶活性为特征的两种新型微生物鲍氏不动杆菌WT-50 / 50-4A（ATCC 202144）和粘质沙雷氏菌WT-L-4A1（RED）（ATCC 202145）。 化合物反式-4-羟基-L-脯氨酸（THLP）或顺式-4-羟基-D-脯氨酸（CHDP）用该差向异构酶活性转化成THLP和CHDP的混合物。 此外，反式-4-羟基-D-脯氨酸（THDP）或顺式-4-羟基-L-脯氨酸（CHLP）的化合物用该差向异构酶活性转化成THDP和CHLP的混合物。

    Perhydrolase providing improved specific activity
    17.
    发明授权
    Perhydrolase providing improved specific activity 失效
    过水解酶提供更好的比活性

    公开(公告)号：US08389255B2

    公开(公告)日：2013-03-05

    申请号：US13070135

    申请日：2011-03-23

    申请人： Robert Dicosimo ,  John Edward Gavagan ,  Mark Scott Payne

    发明人： Robert Dicosimo ,  John Edward Gavagan ,  Mark Scott Payne

    IPC分类号： C12N9/18

    CPC分类号： C12N9/18 ,  C11D3/38636 ,  C12P7/40 ,  C12P7/54

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰热转录海马乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。

    PERHYDROLASE VARIANT PROVIDING IMPROVED SPECIFIC ACTIVITY
    19.
    发明申请
    PERHYDROLASE VARIANT PROVIDING IMPROVED SPECIFIC ACTIVITY 失效
    PERHYDROLASE变体提供改进的特定活性

    公开(公告)号：US20120156162A1

    公开(公告)日：2012-06-21

    申请号：US13328418

    申请日：2011-12-16

    申请人： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

    发明人： Robert Dicosimo ,  Mark Scott Payne ,  John Edward Gavagan

    IPC分类号： A61L9/01 ,  C12N9/16 ,  C12N1/21 ,  D21H21/32 ,  A01N37/02 ,  C07H21/04 ,  D06L3/00 ,  C12P7/62 ,  C12N15/63

    CPC分类号： C12N9/18 ,  C12Y301/01072

    摘要： An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritime acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.

    摘要翻译： 提供具有过水解活性的乙酰木聚糖酯酶变体，用于从羧酸酯和过氧源产生过氧羧酸。 更具体地，使用易错PCR和定点诱变来修饰Thermotoga海洋乙酰木聚糖酯酶基因，以产生特征在于比活性增加的酶催化剂。 变体乙酰木聚糖酯酶可用于制备适用于各种用途的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。