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公开(公告)号：US09623052B2
公开(公告)日：2017-04-18
申请号：US14009018
申请日：2012-03-30
CPC分类号： A61K35/34 , A61K9/70 , A61K35/44 , A61L27/3834 , A61L27/3886 , A61L27/54 , A61L2300/414 , A61L2430/20 , C12N5/0657 , C12N5/0697 , C12N2501/01 , C12N2501/04 , C12N2501/165 , C12N2502/1329 , C12N2502/1347 , C12N2502/28 , C12N2506/02 , A61K2300/00
摘要： The present invention provides a method for producing a myocardial sheet using a group of cells derived from embryonic stem cells. This method is characterized by mixing Flk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells, all derived from embryonic stem cells, and culturing the mixed cells. Furthermore, the myocardial sheet can be used as a therapeutic agent for heart diseases since VEGF is released from the sheet.
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公开(公告)号：US08809053B2
公开(公告)日：2014-08-19
申请号：US12186572
申请日：2008-08-06
IPC分类号： C12N5/07
CPC分类号： C12N5/0657 , C12N2533/30
摘要： Methods and reagents are described for myocardial regenerative therapy using cardiomyocytes that are cultured ex vivo to have a controlled orientation of beating. This is achieved by growing the cardiomyocytes on a culture support that is coated with a polymerizable liquid crystal which is oriented so as to be in a liquid crystal phase state prior to curing with ionizing radiation or ultraviolet rays.
摘要翻译： 描述了使用心肌细胞进行心肌再生治疗的方法和试剂，所述心肌细胞离体培养以具有受控的打浆取向。 这是通过在涂覆有可聚合液晶的培养支持物上生长心肌细胞来实现的，该可聚合液晶在用电离辐射或紫外线固化之前被定向为处于液晶相状态。
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13.发明申请CELL SHEET FOR MYOCARDIAL REGENERATION, METHOD OF PRODUCING THE SAME, AND METHOD OF USING THE SAME 审中-公开用于心肌细胞再生的细胞片及其制备方法及其使用方法公开(公告)号：US20130101659A1
公开(公告)日：2013-04-25
申请号：US13509322
申请日：2010-09-09
申请人： Yoshiki Sawa , Yasuhiro Shudo , Shigeru Miyagawa , Tatsuya Shimizu , Teruo Okano , Akifumi Matsuyama , Atsuhiro Saito
发明人： Yoshiki Sawa , Yasuhiro Shudo , Shigeru Miyagawa , Tatsuya Shimizu , Teruo Okano , Akifumi Matsuyama , Atsuhiro Saito
CPC分类号： A61K35/34 , A61K9/70 , A61K35/12 , A61K35/28 , A61K38/18 , A61K38/19 , A61L27/367 , A61L27/3826 , A61L27/3834 , A61L27/3886 , A61L27/3891 , C12N5/0657 , C12N5/0697 , C12N2502/1329 , C12N2502/1382 , C12N2513/00 , C12N2539/10
摘要： Provided are a cell sheet and a three-dimensional structure thereof that include at least mesenchymal stem cells and myoblasts isolated from a cell culture support and that are to be applied to heart disease. Use of the cell sheet and the three-dimensional structure thereof including mesenchymal stem cells and myoblasts can notably improve cardiac functions compared with a conventional technology, i.e., use of cell sheets composed of myoblasts only or mesenchymal stem cells only. Furthermore, the cell sheet itself has high strength, and the transplantation procedure is also improved.
摘要翻译： 提供一种细胞片及其三维结构，其至少包括间充质干细胞和从细胞培养支持物分离的并且应用于心脏病的成肌细胞。 包括间充质干细胞和成肌细胞在内的细胞片及其三维结构的使用与常规技术相比，可以显着地改善心脏功能，即仅使用由成肌细胞或间充质干细胞构成的细胞片。 此外，细胞片本身具有高强度，并且移植程序也得到改善。
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公开(公告)号：US09617519B2
公开(公告)日：2017-04-11
申请号：US13823007
申请日：2011-09-14
CPC分类号： C12N5/0691 , A61F2/02 , C12M21/08 , C12M25/02 , C12N5/0657 , C12N2502/28 , C12N2513/00 , C12N2533/54 , C12N2533/56
摘要： A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.
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公开(公告)号：US09127270B2
公开(公告)日：2015-09-08
申请号：US13125491
申请日：2009-08-11
CPC分类号： C12N11/02 , C12M23/20 , C12M33/00 , C12N5/0062 , C12N2535/10 , C12N2539/10
摘要： This invention provides a cell pattern recovery tool comprising a base material layer having a surface subjected to easy adhesion treatment, a temperature responsive polymer layer that is provided on the base material layer and has a surface subjected to silane treatment, and a cell adhesion inhibiting material layer provided on the temperature responsive polymer layer. According to the present invention, a cell pattern can be rapidly recovered while maintaining the cell pattern stably and reliably under minimally invasive conditions for the cells.
摘要翻译： 本发明提供了一种电池图案回收工具，其包括具有易于粘附处理的表面的基材层，设置在基材层上并具有进行硅烷处理的表面的温度响应性聚合物层和细胞粘附抑制材料 提供在温度敏感聚合物层上的层。 根据本发明，可以在针对细胞的微创条件下稳定且可靠地保持细胞图案，能够快速地恢复细胞图案。
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公开(公告)号：US20140072599A1
公开(公告)日：2014-03-13
申请号：US14001962
申请日：2012-02-28
申请人： Masahiro Kinooka , Atsuhiro Saito , Yoshiki Sawa , Tatsuya Shimizu , Teruo Okano
发明人： Masahiro Kinooka , Atsuhiro Saito , Yoshiki Sawa , Tatsuya Shimizu , Teruo Okano
CPC分类号： A61K35/33 , A61K35/34 , A61L27/3804 , A61L27/3826 , A61L27/3886 , A61L2300/414 , A61L2300/426 , A61L2300/64 , A61L2430/20 , C12N5/0691 , C12N2502/1323 , C12N2502/1335 , C12N2502/28 , C12N2533/52
摘要： The types and relative proportions of cells constituting a cell sheet stack, and the number of cells to be seeded are altered to change the state of the cells in the cell sheet stack, whereby production of an angiogenesis-promoting cytokine as well as construction of a vascular endothelial network can be optimized.
摘要翻译： 改变构成细胞片叠层的细胞的种类和相对比例，以及待接种的细胞数目，以改变细胞片堆叠中细胞的状态，由此产生促血管生成细胞因子以及 血管内皮网可以优化。
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17.发明申请METHOD FOR INDUCING DIFFERENTIATION OF EMBRYONIC STEM CELLS OR ARTIFICIAL PLURIPOTENT STEM CELLS 审中-公开诱导胚胎干细胞或人工细胞干细胞分化的方法公开(公告)号：US20120107930A1
公开(公告)日：2012-05-03
申请号：US13320777
申请日：2010-05-21
申请人： Daisuke Sasaki , Tatsuya Shimizu , Teruo Okano
发明人： Daisuke Sasaki , Tatsuya Shimizu , Teruo Okano
IPC分类号： C12N5/071
CPC分类号： A61L27/3834 , A61K35/12 , A61L27/16 , A61L2430/20 , C12N5/0657 , C12N2506/02 , C12N2513/00 , C12N2535/10 , C12N2539/10 , C08L33/00
摘要： Differentiation of a large number of embryonic stem cells or artificial pluripotent stem cells such as induced pluripotent stem cells can be readily induced by the following method. A substrate surface having a cell non-adhesive region on the periphery of a cell adhesive region is used, and (1) the cells are allowed to adhere to the cell adhesive region of the substrate surface in a differentiation-inducing medium; (2) the cells are subsequently allowed to grow on the cell adhesive surface to reach confluence; (3) the culture is further continued to three-dimensionally stratify the cells from the boundary with the cell non-adhesive region surrounding the cell adhesive region; and (4) a plurality of the stratified portions formed in the cell adhesive region is finally bound to one another to produce a cell agglomerate spreading over the entire cell adhesive region with a certain thickness.
摘要翻译： 通过以下方法可以容易地诱导大量胚胎干细胞或人造多能干细胞如诱导多能干细胞的分化。 使用在细胞粘附区域的周围具有细胞非粘合区域的基板表面，（1）允许细胞在分化诱导介质中粘附到基板表面的细胞粘附区域; （2）随后使细胞在细胞粘附剂表面上生长以达到汇合; （3）进一步将培养物与细胞粘附区域周围的细胞非粘合区域从边界三维地分层化， 和（4）形成在电池粘合剂区域中的多个分层部分最终彼此结合以产生在整个电池粘合剂区域上以一定厚度铺展的电池团聚体。
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18.发明申请公开(公告)号：US20120052524A1
公开(公告)日：2012-03-01
申请号：US13254040
申请日：2010-03-04
申请人： Masahiro Kinooka , Yasunori Takezawa , Masahito Taya , Atsuhiro Saito , Yoshiki Sawa , Tatsuya Shimizu , Teruo Okano
发明人： Masahiro Kinooka , Yasunori Takezawa , Masahito Taya , Atsuhiro Saito , Yoshiki Sawa , Tatsuya Shimizu , Teruo Okano
CPC分类号： G01N33/5008 , C12M41/46 , C12Q1/025 , G01N33/5026 , G01N33/5029 , G01N33/5064
摘要： Three-dimensional information on a biological parameter relating to at least one selected from viability, proliferating ability, migration, and differentiation of cultured cells can be obtained by a simple two-dimensional analytical technique by constructing a cell evaluation system including a multilayered cell sheet, target cells, and a two-dimensional analyzer.
摘要翻译： 通过简单的二维分析技术，可以通过构建包括多层细胞片的细胞评估系统来获得关于选自生殖力，增殖能力，迁移和培养细胞分化中的至少一种的生物学参数的三维信息， 靶细胞和二维分析仪。
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19.发明授权公开(公告)号：US08916189B2
公开(公告)日：2014-12-23
申请号：US12122194
申请日：2008-05-16
CPC分类号： C12N5/0657 , C12N5/0068 , C12N2533/30 , C12N2535/10
摘要： This invention is intended to provide cell aggregates that can reproduce functions of the myocardium, such as the function of beating, and thus are available for myocardial regenerative therapy, and to provide a cell culture support for producing the same.According to this invention, a cell culture support for forming string-shaped cardiomyocyte aggregates, which has a substrate portion having one surface on which at least one linear cell-adherent region is formed is provided.
摘要翻译： 本发明旨在提供可以再现心肌功能的细胞聚集体，例如打浆的功能，因此可用于心肌再生治疗，并提供用于制备心肌再生治疗的细胞培养支持物。 根据本发明，提供了一种用于形成丝状心肌细胞聚集体的细胞培养支持体，其具有其上形成有至少一个线性细胞粘附区域的一个表面的基底部分。
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公开(公告)号：US08821815B2
公开(公告)日：2014-09-02
申请号：US13542459
申请日：2012-07-05
CPC分类号： C12M45/02
摘要： A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out.
摘要翻译： 电池隔离器包括：第一容器，其在上部具有开口; 限定所述第一容器的下部的至少一部分的过滤构件; 隔离构件，其可移动地容纳在第一容器中以与组织碰撞，从而隔离单元; 以及容纳第一容器的第二容器，其中第一容器可以被取出。
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