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公开(公告)号：US20140056859A1
公开(公告)日：2014-02-27
申请号：US14009018
申请日：2013-03-30
CPC分类号： A61K35/34 , A61K9/70 , A61K35/44 , A61L27/3834 , A61L27/3886 , A61L27/54 , A61L2300/414 , A61L2430/20 , C12N5/0657 , C12N5/0697 , C12N2501/01 , C12N2501/04 , C12N2501/165 , C12N2502/1329 , C12N2502/1347 , C12N2502/28 , C12N2506/02 , A61K2300/00
摘要： The present invention provides a method for producing a myocardial sheet using a group of cells derived from embryonic stem cells. This method is characterized by mixing Flk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells, all derived from embryonic stem cells, and culturing the mixed cells. Furthermore, the myocardial sheet can be used as a therapeutic agent for heart diseases since VEGF is released from the sheet.
摘要翻译： 本发明提供使用源自胚胎干细胞的一组细胞产生心肌片的方法。 该方法的特征在于混合来自胚胎干细胞的Flk / KDR阳性细胞，心肌细胞，内皮细胞和壁细胞，并培养混合细胞。 此外，心肌片可用作心脏病的治疗剂，因为VEGF从片材中释放出来。
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公开(公告)号：US09623052B2
公开(公告)日：2017-04-18
申请号：US14009018
申请日：2012-03-30
CPC分类号： A61K35/34 , A61K9/70 , A61K35/44 , A61L27/3834 , A61L27/3886 , A61L27/54 , A61L2300/414 , A61L2430/20 , C12N5/0657 , C12N5/0697 , C12N2501/01 , C12N2501/04 , C12N2501/165 , C12N2502/1329 , C12N2502/1347 , C12N2502/28 , C12N2506/02 , A61K2300/00
摘要： The present invention provides a method for producing a myocardial sheet using a group of cells derived from embryonic stem cells. This method is characterized by mixing Flk/KDR positive cells, cardiomyocytes, endothelial cells, and mural cells, all derived from embryonic stem cells, and culturing the mixed cells. Furthermore, the myocardial sheet can be used as a therapeutic agent for heart diseases since VEGF is released from the sheet.
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3.发明授权Method for manufacturing multilayered cell sheet, multilayered cell sheet having vascular network obtained thereby, and method of use thereof 有权制造多层细胞片的方法，由此获得的具有血管网的多层细胞片及其使用方法公开(公告)号：US09289454B2
公开(公告)日：2016-03-22
申请号：US13822945
申请日：2011-09-14
申请人： Hidekazu Sekine , Tatsuya Shimizu , Teruo Okano
发明人： Hidekazu Sekine , Tatsuya Shimizu , Teruo Okano
CPC分类号： A61K35/44 , A61K35/34 , A61K35/36 , A61L27/3604 , A61L27/3839 , A61L27/3895 , C12N5/0657 , C12N2502/28 , C12N2513/00
摘要： A method for producing multilayered cell sheets, including producing a vascular bed which includes an artery-vein loop and in which a capillary vascular network is constructed; layering cell sheets on the vascular bed; and perfusing a culture medium in vitro to construct a vascular network in the cell sheets. The production method enables vascular networks to be constructed in cell sheets and enables thick multilayered cell sheets to foe easily produced by layering the cell sheets. Such thick multilayered cell sheets are useful as in vivo tissue-like products for regenerative medicine for various tissues and for evaluation of drugs and the like.
摘要翻译： 一种生产多层细胞片的方法，包括制备包括动静脉环的血管床，其中构成毛细血管网络; 在血管床上分层细胞片; 并在体外灌注培养基以在细胞片中构建血管网络。 该制造方法能够在细胞片中构建血管网络，能够通过层叠细胞片容易地制造厚层多层细胞片。 这种厚的多层细胞片可用作用于各种组织的再生医学的体内组织样产品和药物等的评估。
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公开(公告)号：US20120214250A1
公开(公告)日：2012-08-23
申请号：US13400106
申请日：2012-02-19
申请人： Mitsuhiro Oura , Teruo Okano , Tatsuya Shimizu , Hirotsugu Kubo , Sunao Takeda , Shinji Yamamori
发明人： Mitsuhiro Oura , Teruo Okano , Tatsuya Shimizu , Hirotsugu Kubo , Sunao Takeda , Shinji Yamamori
IPC分类号： G01N21/59
CPC分类号： G01N21/80 , C12M41/26 , G01N21/3151 , G01N31/221 , G01N2201/0627 , G01N2201/1211
摘要： A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances ; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.
摘要翻译： 测量溶液的pH的方法包括：在测量区域中的溶液脉动的同时，从混合有指示剂的溶液的测量区域的一侧发射两个波长的光束; 在所述测量区域中的所述溶液脉动的同时，在测量区域的另一侧上接收发射光束的透射光束和反射光束中的至少一个; 基于所接收的所述透射光束和所述反射光束中的至少一个，获得所述两个波长的吸光度; 从所获得的吸光度获得吸光度比; 并且基于所获得的吸光度比和预先存储的吸光度比/ pH值对应数据库来计算溶液的pH值。
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公开(公告)号：US20110207220A1
公开(公告)日：2011-08-25
申请号：US13125491
申请日：2009-08-11
IPC分类号： C12N5/071
CPC分类号： C12N11/02 , C12M23/20 , C12M33/00 , C12N5/0062 , C12N2535/10 , C12N2539/10
摘要： This invention provides a cell pattern recovery tool comprising a base material layer having a surface subjected to easy adhesion treatment, a temperature responsive polymer layer that is provided on the base material layer and has a surface subjected to silane treatment, and a cell adhesion inhibiting material layer provided on the temperature responsive polymer layer. According to the present invention, a cell pattern can be rapidly recovered while maintaining the cell pattern stably and reliably under minimally invasive conditions for the cells.
摘要翻译： 本发明提供了一种电池图案回收工具，其包括具有易于粘附处理的表面的基材层，设置在基材层上并具有进行硅烷处理的表面的温度响应性聚合物层和细胞粘附抑制材料 提供在温度敏感聚合物层上的层。 根据本发明，可以在针对细胞的微创条件下稳定且可靠地保持细胞图案，能够快速地恢复细胞图案。
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公开(公告)号：US09304088B2
公开(公告)日：2016-04-05
申请号：US13400106
申请日：2012-02-19
申请人： Mitsuhiro Oura , Teruo Okano , Tatsuya Shimizu , Hirotsugu Kubo , Sunao Takeda , Shinji Yamamori
发明人： Mitsuhiro Oura , Teruo Okano , Tatsuya Shimizu , Hirotsugu Kubo , Sunao Takeda , Shinji Yamamori
CPC分类号： G01N21/80 , C12M41/26 , G01N21/3151 , G01N31/221 , G01N2201/0627 , G01N2201/1211
摘要： A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.
摘要翻译： 测量溶液的pH的方法包括：在测量区域中的溶液脉动的同时，从混合有指示剂的溶液的测量区域的一侧发射两个波长的光束; 在所述测量区域中的所述溶液脉动的同时，在所述测量区域的另一侧上接收所发射的光束的透射光束和反射光束中的至少一个; 基于所接收的所述透射光束和所述反射光束中的至少一个，获得所述两个波长的吸光度; 从所获得的吸光度获得吸光度比; 并且基于所获得的吸光度比和预先存储的吸光度比/ pH值对应数据库来计算溶液的pH值。
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公开(公告)号：US08809053B2
公开(公告)日：2014-08-19
申请号：US12186572
申请日：2008-08-06
IPC分类号： C12N5/07
CPC分类号： C12N5/0657 , C12N2533/30
摘要： Methods and reagents are described for myocardial regenerative therapy using cardiomyocytes that are cultured ex vivo to have a controlled orientation of beating. This is achieved by growing the cardiomyocytes on a culture support that is coated with a polymerizable liquid crystal which is oriented so as to be in a liquid crystal phase state prior to curing with ionizing radiation or ultraviolet rays.
摘要翻译： 描述了使用心肌细胞进行心肌再生治疗的方法和试剂，所述心肌细胞离体培养以具有受控的打浆取向。 这是通过在涂覆有可聚合液晶的培养支持物上生长心肌细胞来实现的，该可聚合液晶在用电离辐射或紫外线固化之前被定向为处于液晶相状态。
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8.发明申请CELL SHEET FOR MYOCARDIAL REGENERATION, METHOD OF PRODUCING THE SAME, AND METHOD OF USING THE SAME 审中-公开用于心肌细胞再生的细胞片及其制备方法及其使用方法公开(公告)号：US20130101659A1
公开(公告)日：2013-04-25
申请号：US13509322
申请日：2010-09-09
申请人： Yoshiki Sawa , Yasuhiro Shudo , Shigeru Miyagawa , Tatsuya Shimizu , Teruo Okano , Akifumi Matsuyama , Atsuhiro Saito
发明人： Yoshiki Sawa , Yasuhiro Shudo , Shigeru Miyagawa , Tatsuya Shimizu , Teruo Okano , Akifumi Matsuyama , Atsuhiro Saito
CPC分类号： A61K35/34 , A61K9/70 , A61K35/12 , A61K35/28 , A61K38/18 , A61K38/19 , A61L27/367 , A61L27/3826 , A61L27/3834 , A61L27/3886 , A61L27/3891 , C12N5/0657 , C12N5/0697 , C12N2502/1329 , C12N2502/1382 , C12N2513/00 , C12N2539/10
摘要： Provided are a cell sheet and a three-dimensional structure thereof that include at least mesenchymal stem cells and myoblasts isolated from a cell culture support and that are to be applied to heart disease. Use of the cell sheet and the three-dimensional structure thereof including mesenchymal stem cells and myoblasts can notably improve cardiac functions compared with a conventional technology, i.e., use of cell sheets composed of myoblasts only or mesenchymal stem cells only. Furthermore, the cell sheet itself has high strength, and the transplantation procedure is also improved.
摘要翻译： 提供一种细胞片及其三维结构，其至少包括间充质干细胞和从细胞培养支持物分离的并且应用于心脏病的成肌细胞。 包括间充质干细胞和成肌细胞在内的细胞片及其三维结构的使用与常规技术相比，可以显着地改善心脏功能，即仅使用由成肌细胞或间充质干细胞构成的细胞片。 此外，细胞片本身具有高强度，并且移植程序也得到改善。
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公开(公告)号：US09617519B2
公开(公告)日：2017-04-11
申请号：US13823007
申请日：2011-09-14
CPC分类号： C12N5/0691 , A61F2/02 , C12M21/08 , C12M25/02 , C12N5/0657 , C12N2502/28 , C12N2513/00 , C12N2533/54 , C12N2533/56
摘要： A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.
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公开(公告)号：US09291572B2
公开(公告)日：2016-03-22
申请号：US13441630
申请日：2012-04-06
申请人： Mitsuhiro Oura , Hirotsugu Kubo , Sunao Takeda , Teruo Okano , Tatsuya Shimizu
发明人： Mitsuhiro Oura , Hirotsugu Kubo , Sunao Takeda , Teruo Okano , Tatsuya Shimizu
CPC分类号： G01N21/80 , C12M41/26 , G01N31/221 , G01N2021/3148 , G01N2021/3181 , G01N2201/0627
摘要： A pH measuring method includes: illuminating a medium solution, which includes: a first material; and a second material, with a plurality of light beams, which includes: a first light beam, a wavelength of which corresponds to a first absorption peak; a second light beam, a wavelength of which corresponds to a second absorption peak or a second convergence point; a third light beam, a wavelength of which corresponds to a third absorption peak or a third convergence point; and a fourth light beam, a wavelength of the fourth light beam at which an absorbance of at least one of the first and second materials is converged irrespective of a pH; receiving transmitted or reflected light beams of the first to fourth light beams; measuring absorbances at the wavelengths of the received light beams respectively; and calculating a pH of the medium solution based on the absorbances.
摘要翻译： pH测量方法包括：照射介质溶液，其包括：第一材料; 以及具有多个光束的第二材料，其包括：第一光束，其波长对应于第一吸收峰; 第二光束，其波长对应于第二吸收峰或第二会聚点; 第三光束，其波长对应于第三吸收峰或第三会聚点; 以及第四光束，所述第四光束的波长，其中所述第一和第二材料中的至少一个的吸光度在不同pH下会聚; 接收第一至第四光束的透射或反射光束; 分别测量接收光束波长处的吸光度; 并基于吸光度计算介质溶液的pH。
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