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    PRODUCTION METHOD OF MYOCARDIAL CELL LAYER, MYOCARDIAL CELL LAYER, AND USE THEREOF
    15.
    发明公开
    PRODUCTION METHOD OF MYOCARDIAL CELL LAYER, MYOCARDIAL CELL LAYER, AND USE THEREOF 审中-公开

    公开(公告)号：US20240277899A1

    公开(公告)日：2024-08-22

    申请号：US18648087

    申请日：2024-04-26

    Applicant: FUJIFILM Corporation ,  OSAKA UNIVERSITY

    Inventor： Hayato MIYOSHI ,  Li LIU ,  Yoshiki SAWA ,  Junjun LI ,  Shigeru MIYAGAWA ,  Maki TAKEDA

    IPC: A61L27/38 ,  A61K35/34 ,  C12N5/077

    CPC classification number: A61L27/38 ,  A61K35/34 ,  C12N5/0657 ,  C12N2502/1323

    Abstract: An object of the present invention is to provide a production method of a myocardial cell layer, and a myocardial cell layer, in which myocardial cells can be matured without using a complicated control device and can be maintained for a long period of time, and to provide a kit for evaluating a pharmaceutical candidate substance, a transplantation material, and an evaluation method of a pharmaceutical candidate substance. According to the present invention, there is provided a production method of a myocardial cell layer including the following steps (1) to (3).



    (1) seeding a myocardial cell induced from a pluripotent stem cell in a single layer on a seeding surface having a shape shown in the following (1-1) to form a myocardial cell layer,
    (1-1) in a case where spread of the seeding surface in a horizontal direction is a plane figure, a shape thereof is a shape obtained by hollowing out at least a part of a first plane figure of an optional shape having uniform spread in a second plane figure of another optional shape having uniform spread without contacting an outer periphery of the first plane figure of the optional shape, where an average value of a length of the outer periphery of the first plane figure and a length of an outer periphery of the second plane figure satisfies the following formula;










    in the formula, x represents the average value of the length of the outer periphery of the first plane figure and the length of the outer periphery of the second plane figure, where a unit thereof is cm, and v represents a conduction velocity of the myocardial cell induced from the pluripotent stem cell, where a unit thereof is cm·s−1,
    (2) circulating and proceeding an electrical signal or a calcium signal in the myocardial cell layer, and
    (3) culturing the cells for 8 days or more while maintaining a state of the (2).

    METHOD FOR PRODUCING FIBRIN SHEET
    17.
    发明申请
    METHOD FOR PRODUCING FIBRIN SHEET 有权

    公开(公告)号：US20220372437A1

    公开(公告)日：2022-11-24

    申请号：US17765637

    申请日：2020-09-09

    Applicant: OSAKA UNIVERSITY

    Inventor： Takaaki SAMURA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Junya YOKOYAMA

    IPC: C12N5/00 ,  C07K14/75

    Abstract: A method for producing a fibrin sheet containing at least one selected from the group consisting of cells and drugs in a fibrin gel, the method comprising: a step 1 of applying a fibrinogen solution containing at least one selected from the group consisting of cells and drugs and fibrinogen dropwise onto a surface of a substrate made of a gelatin hydrogel; a step 2 of adding thrombin to the fibrinogen solution on the surface of the substrate; a step 3 of placing a support film on and in contact with a top surface of the fibrinogen solution to which the thrombin has been added; a step 4 of forming a fibrin sheet containing the at least one selected from the group consisting of cells and drugs in a fibrin gel between the substrate and the support film by a reaction between the fibrinogen and the thrombin; and a step 5 of melting the substrate at a temperature not lower than a melting temperature of the gelatin hydrogel to separate, from the substrate, the fibrin sheet supported by the support film.

    MYOCARDIAL CELL SHEET
    18.
    发明申请
    MYOCARDIAL CELL SHEET 审中-公开

    公开(公告)号：US20170247658A1

    公开(公告)日：2017-08-31

    申请号：US15594011

    申请日：2017-05-12

    Applicant: TERUMO KABUSHIKI KAISHA ,  OSAKA UNIVERSITY

    Inventor： Hiroko ISEOKA ,  Yoshiki SAWA ,  Shigeru MIYAGAWA ,  Satsuki FUKUSHIMA

    IPC: C12N5/077 ,  A61K35/34

    CPC classification number: C12N5/0657 ,  A61K35/12 ,  A61K35/34 ,  A61L27/00 ,  C12N2506/45

    Abstract: A sheet-shaped cell culture is disclosed that includes myocardial cells derived from pluripotent stem cells and has excellent functional properties. A sheet-shaped cell culture obtained by culturing a cell population that includes myocardial cells derived from pluripotent stem cells, wherein the proportion of the number of the myocardial cells derived from pluripotent stem cells to the total number of cells in the cell population is 50% to 70%; a method of producing the sheet-shaped cell culture; a composition including the sheet-shaped cell culture; and a method of treating a disease using the sheet-shaped cell culture or the composition.

    METHOD FOR PRODUCING LAMINATE OF SHEET-SHAPED CELL CULTURE AND FIBRIN GEL
    20.
    发明申请
    METHOD FOR PRODUCING LAMINATE OF SHEET-SHAPED CELL CULTURE AND FIBRIN GEL 有权
    用于生产片状细胞培养物和纤维蛋白凝胶层压体的方法

    公开(公告)号：US20160058908A1

    公开(公告)日：2016-03-03

    申请号：US14844259

    申请日：2015-09-03

    Applicant: TERUMO KABUSHIKI KAISHA ,  OSAKA UNIVERSITY

    Inventor： Fumiya OOHASHI ,  Ryouhei TAKEUCHI ,  Tadashi SAMESHIMA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Mitsuhiro SAITO

    IPC: A61L27/34 ,  A61L27/58 ,  A61L27/38

    CPC classification number: A61L27/58 ,  A61L27/225 ,  A61L27/38 ,  A61L27/52 ,  A61L2430/20

    Abstract: A laminate of a sheet-shaped cell culture is disclosed, which has excellent operability and is suitable for implantation. A method is disclosed for producing a laminate of a fibrin gel and a sheet-shaped cell culture, including a step of dripping a liquid containing fibrinogen onto an upper surface of a sheet-shaped cell culture, a step of spraying a liquid containing thrombin onto the surface, and a step of forming a fibrin gel layer on the surface by a reaction between fibrinogen and thrombin; and a laminate of a fibrin gel and a sheet-shaped cell culture produced by the method are disclosed.

    Abstract translation: 公开了一种片状细胞培养物的层压体，其具有优异的可操作性并且适于植入。 公开了用于制造纤维蛋白凝胶和片状细胞培养物的层压体的方法，包括将含有纤维蛋白原的液体滴落到片状细胞培养物的上表面的步骤，将含有凝血酶的液体喷雾到 表面和通过纤维蛋白原和凝血酶之间的反应在表面上形成纤维蛋白凝胶层的步骤; 并且公开了通过该方法制备的纤维蛋白凝胶和片状细胞培养物的层压体。

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