公开(公告)号：US12247219B2

公开(公告)日：2025-03-11

申请号：US17348174

申请日：2021-06-15

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Yuki Ishikawa

IPC: C12N5/079 ,  C12N5/074 ,  C12N5/10

Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.

公开(公告)号：US12013365B2

公开(公告)日：2024-06-18

申请号：US17258662

申请日：2019-07-18

Applicant: OSAKA UNIVERSITY

Inventor： Kohji Nishida ,  Noriyasu Hashida ,  Masateru Taniguchi ,  Makusu Tsutsui

IPC: G01N27/327 ,  G01N27/447 ,  G01N33/487 ,  G01N33/569

CPC classification number: G01N27/3278 ,  G01N27/447 ,  G01N33/48721 ,  G01N33/56983

Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.

公开(公告)号：US11839697B2

公开(公告)日：2023-12-12

申请号：US16621528

申请日：2018-06-19

Applicant: Osaka University

Inventor： Kohji Nishida ,  Motokazu Tsujikawa ,  Susumu Hara ,  Satoshi Kawasaki ,  Masahito Yoshihara ,  Masayoshi Itoh ,  Hideya Kawaji

IPC: A61L27/38 ,  C12Q1/06 ,  C12Q1/6869 ,  A61K35/545 ,  C12N5/079 ,  A61K35/30 ,  C12Q1/6883 ,  A61K35/28

CPC classification number: A61L27/3808 ,  A61K35/30 ,  A61K35/545 ,  A61L27/38 ,  C12N5/0621 ,  C12Q1/06 ,  C12Q1/6869 ,  A61K35/28 ,  C12Q1/6883 ,  C12Q2600/158

Abstract: Provided is a means for identifying cells suitable for transplantation into a cornea, or a means for producing a cell population suitable for transplantation into a cornea using the same. A method for producing a cell population suitable for transplantation into a cornea, the method comprising the steps of (1) preparing a cell population cultured under conditions suitable for inducing differentiation into corneal endothelial cells, and (2) measuring the expression of at least one gene selected from the group consisting of POU6F2, LMX1B, and TFAP2B in the cell population.

公开(公告)号：US11649433B2

公开(公告)日：2023-05-16

申请号：US16482160

申请日：2018-01-31

Applicant: Osaka University

Inventor： Kohji Nishida ,  Kiyotoshi Sekiguchi ,  Ryuhei Hayashi ,  Shun Shibata

IPC: A61K35/545 ,  C12N5/079 ,  A61K38/18

CPC classification number: C12N5/0621 ,  A61K35/545 ,  A61K38/1825 ,  A61K38/18 ,  C12N2501/998 ,  C12N2506/45 ,  C12N2533/52

Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.

公开(公告)号：US20190119747A1

公开(公告)日：2019-04-25

申请号：US16089317

申请日：2017-03-28

Applicant: Osaka University

Inventor： Kohji Nishida ,  Atsushi Kumanogoh ,  Noriyasu Hashida

IPC: C12Q1/6876

Abstract: The principal purpose of the present invention is to provide a novel stress biomarker that makes it possible to conveniently and accurately assess a state of stress. In addition, the present invention provides a diagnosis kit containing a reagent capable of detecting said biomarker, and a diagnosis method that uses said biomarker. It is possible to use mitochondrial DNA included in a biological fluid as the stress biomarker.

公开(公告)号：US20180148685A1

公开(公告)日：2018-05-31

申请号：US15543021

申请日：2016-01-12

Applicant: Osaka University

Inventor： Kohji Nishida ,  Yuzuru SASAMOTO ,  Ryuhei HAYASHI

IPC: C12N5/071 ,  C12N15/86 ,  C07K14/47

Abstract: The present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell from a surface ectoderm-derived cell. More specifically, the present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell, comprising introducing PAX6, KLF4, and OCT4 into a surface ectoderm-derived cell, such as an oral mucosal epithelial cell, and a corneal epithelioid cell induced by the method.

公开(公告)号：US12202218B2

公开(公告)日：2025-01-21

申请号：US17254120

申请日：2019-06-20

Applicant: OSAKA UNIVERSITY

Inventor： Kohji Nishida ,  Koichi Baba ,  Shizuka Koh ,  Kei Ohkubo ,  Haruyasu Asahara ,  Tsuyoshi Inoue ,  Hiroshi Uyama ,  Taka-Aki Asoh

IPC: B29D11/00 ,  A61F2/16 ,  C08L101/12

Abstract: The present invention provides a new method for producing a lens to be fitted to an eye in a simple manner at a low cost. In order to achieve the aforementioned object, the present invention provides a method for producing a lens to be fitted to an eye, including the step of: hydrophilizing a surface of a polymer lens by reacting with a compound radical, wherein the compound radical is a radical containing one element selected from the group consisting of Group 15 elements and Group 16 elements, and a Group 17 element.

公开(公告)号：US12035973B2

公开(公告)日：2024-07-16

申请号：US16964029

申请日：2019-01-25

Applicant: OSAKA UNIVERSITY

Inventor： Kohji Nishida ,  Kazuichi Maruyama ,  Noriyasu Hashida ,  Reiko Kobayashi

IPC: A61B3/14 ,  A61B3/10 ,  A61B5/16

CPC classification number: A61B3/14 ,  A61B3/102 ,  A61B5/165

Abstract: A method for detecting a stressed state, including a step of detecting a stressed state of a subject based on a tomographic image of a choroid of the subject, and a stress detection apparatus including: an image acquisition unit that acquires a tomographic image of a choroid of a subject; a calculation unit that calculates a choroidal thickness or a volume of the choroid based on the tomographic image; and a detection unit that detects a stressed state of the subject based on the choroidal thickness or the volume of the choroid.

公开(公告)号：US20210332324A1

公开(公告)日：2021-10-28

申请号：US16476521

申请日：2017-12-27

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Shun Shibata

IPC: C12N5/079 ,  A61L27/38

Abstract: The present invention provides a method for producing a corneal epithelial cell population for the fabrication of corneal epithelial cell sheets for transplantation, the method comprising the steps of: (1) preparing a cell population containing corneal epithelial cells; (2) subjecting the cell population prepared in step (1) to magnetic-activated cell sorting and collecting CD200-negative/SSEA-4-positive cells; and (3) bringing the cells collected in step (2) into contact with a laminin selected from the group consisting of a laminin having an α3 chain, a laminin having an α4 chain and a laminin having an α5 chain, or an E8 fragment thereof, and collecting cells adherent thereto.

公开(公告)号：US20210207087A1

公开(公告)日：2021-07-08

申请号：US17057021

申请日：2019-05-22

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Kimihito Nomi

IPC: C12N5/079 ,  G01N33/50

Abstract: Provided is a method for inducing differentiation into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells, the method comprising culturing colonies of pluripotent stem cells in a medium containing an epidermal growth factor (EGF) signaling activator to induce differentiation of the pluripotent stem cells. The present invention enables differentiation of pluripotent stem cells including iPS cells into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells and therefore is very useful for, for example, basic research on conjunctival epithelial cells and conjunctival goblet cells, regenerative therapy for intractable ocular surface diseases, and research related to the diseases.