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22 条记录 (耗时 0.032 秒)

    Methods of surface modification of a flexible substrate to enhance cell adhesion
    11.
    发明申请
    Methods of surface modification of a flexible substrate to enhance cell adhesion 有权
    柔性基材的表面改性方法，以增强细胞粘附力

    公开(公告)号：US20050058842A1

    公开(公告)日：2005-03-17

    申请号：US10660760

    申请日：2003-09-12

    申请人： Andrea Liebmann-Vinson ,  Bryce Chaney

    发明人： Andrea Liebmann-Vinson ,  Bryce Chaney

    IPC分类号： B05D1/18 ,  B05D3/12 ,  C12M3/00 ,  C12N5/00 ,  B32B25/20

    CPC分类号： B82Y30/00 ,  B05D1/185 ,  B05D3/12 ,  B05D2201/00 ,  C12M25/14 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50 ,  Y10T428/31663

    摘要： The present invention provides methods of producing a surface with enhanced cell-adhesive properties comprising applying a stress to a polymeric matrix. The strained matrix is then modified by grafting a self-assembled monolayer onto the strained matrix, with the self-assembled monolayer comprising at least one exposed functional group. At least one cell-adhesive molecule can then be coupled to the at least one exposed functional group on the self-assembled monolayer to produce a surface with enhanced cell-adhesive properties.

    摘要翻译： 本发明提供了生产具有增强的电池粘合性能的表面的方法，包括向聚合物基质施加应力。 然后通过将自组装单层接枝到应变基质上来修饰应变基质，其中自组装单层包含至少一个暴露的官能团。 然后可以将至少一个细胞粘附分子与自组装单层上的至少一个暴露的官能团偶联以产生具有增强的细胞粘附性质的表面。

    Methods of surface modification to enhance cell adhesion
    13.
    发明申请
    Methods of surface modification to enhance cell adhesion 审中-公开
    表面改性方法增强细胞粘附力

    公开(公告)号：US20050059140A1

    公开(公告)日：2005-03-17

    申请号：US10660759

    申请日：2003-09-12

    申请人： Andrea Liebmann-Vinson ,  Bryce Chaney

    发明人： Andrea Liebmann-Vinson ,  Bryce Chaney

    IPC分类号： C12N5/00 ,  C12N11/06 ,  C12N11/16 ,  G01N33/543

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12N5/0068 ,  C12N11/06 ,  C12N11/16 ,  C12N2533/30 ,  C12N2533/52 ,  G01N33/54353 ,  G01N33/54393 ,  G01N2610/00

    摘要： The current invention relates to methods of producing a surface with enhanced cell-adhesive properties comprising treating a pre-formed surface such that at least one intermediate reactive group is exposed on the surface. The exposed intermediate reactive group is then reacted to create a self-assembled monolayer, comprising at least one reactive group. The self-assembled monolayer is created non-mechanically. The reactive group is then coupled to at least one cell-adhesive molecule. The current invention also relates to cell culture devices comprising at least one oxygen-sensing compound and a cell-adhesive molecule.

    摘要翻译： 本发明涉及生产具有增强的电池 - 粘合性能的表面的方法，包括处理预成形表面，使得至少一个中间反应性基团暴露在表面上。 然后暴露的中间反应性基团反应以产生包含至少一个反应性基团的自组装单层。 自组装单层是非机械的。 然后将反应性基团偶联至至少一种细胞粘附分子。 本发明还涉及包含至少一种氧传感化合物和细胞粘附分子的细胞培养装置。

    Environments that maintain function of primary liver cells
    14.
    发明申请
    Environments that maintain function of primary liver cells 审中-公开
    维持原发性肝细胞功能的环境

    公开(公告)号：US20070092861A1

    公开(公告)日：2007-04-26

    申请号：US10570702

    申请日：2004-09-10

    申请人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammed Heidaran ,  John Hemperly ,  Stewart Jurgensen ,  Ronald Laethem ,  Neil Robbins

    发明人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammed Heidaran ,  John Hemperly ,  Stewart Jurgensen ,  Ronald Laethem ,  Neil Robbins

    IPC分类号： C12Q1/00 ,  C12N5/08

    CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, lamninin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

    摘要翻译： 用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，lamninin，胶原I，胶原 III，胶原IV和胶原蛋白VI。 此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL）， 聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。 该表面用于细胞培养以促进细胞附着，存活，功能的维持和/或原代肝细胞的增殖。 本发明还涉及利用该表面的方法，例如附着，存活，维持功能和/或细胞增殖的方法。 还公开了使用无血清培养基的细胞培养物中的表面。 还公开了使用本发明的表面进行筛选的方法。