利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.046 秒)

    Environments that maintain function of primary liver cells
    1.
    发明申请
    Environments that maintain function of primary liver cells 审中-公开
    维持原发性肝细胞功能的环境

    公开(公告)号：US20070092861A1

    公开(公告)日：2007-04-26

    申请号：US10570702

    申请日：2004-09-10

    申请人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammed Heidaran ,  John Hemperly ,  Stewart Jurgensen ,  Ronald Laethem ,  Neil Robbins

    发明人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammed Heidaran ,  John Hemperly ,  Stewart Jurgensen ,  Ronald Laethem ,  Neil Robbins

    IPC分类号： C12Q1/00 ,  C12N5/08

    CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, lamninin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

    摘要翻译： 用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，lamninin，胶原I，胶原 III，胶原IV和胶原蛋白VI。 此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL）， 聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。 该表面用于细胞培养以促进细胞附着，存活，功能的维持和/或原代肝细胞的增殖。 本发明还涉及利用该表面的方法，例如附着，存活，维持功能和/或细胞增殖的方法。 还公开了使用无血清培养基的细胞培养物中的表面。 还公开了使用本发明的表面进行筛选的方法。

    Covalently attached collagen VI for cell attachment and proliferation
    2.
    发明申请
    Covalently attached collagen VI for cell attachment and proliferation 审中-公开
    共价连接胶原蛋白VI用于细胞附着和增殖

    公开(公告)号：US20050058687A1

    公开(公告)日：2005-03-17

    申请号：US10660781

    申请日：2003-09-12

    申请人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    发明人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08 ,  G01N33/543

    CPC分类号： G01N33/54393 ,  C12N5/0068 ,  C12N2500/90 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

    摘要翻译： 用于细胞培养物的表面包括与CAR材料结合的载体，并结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体） ），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。 此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。 该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系 ）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。 还公开了包含表面和附加试剂的试剂盒。

    Covalently attached collagen VI for cell attachment and proliferation
    3.
    发明申请
    Covalently attached collagen VI for cell attachment and proliferation 审中-公开
    共价连接胶原蛋白VI用于细胞附着和增殖

    公开(公告)号：US20060105455A1

    公开(公告)日：2006-05-18

    申请号：US11322637

    申请日：2005-12-30

    申请人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    发明人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    IPC分类号： C12N5/08

    CPC分类号： G01N33/54393 ,  C12N5/0068 ,  C12N2500/90 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

    摘要翻译： 用于细胞培养物的表面包括与CAR材料结合的载体，并且结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体） ），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。 此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。 该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系 ）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。 还公开了包含表面和附加试剂的试剂盒。

    Environments that maintain function of primary liver cells
    4.
    发明申请
    Environments that maintain function of primary liver cells 审中-公开
    维持原发性肝细胞功能的环境

    公开(公告)号：US20050059150A1

    公开(公告)日：2005-03-17

    申请号：US10664037

    申请日：2003-09-17

    申请人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammad Heidaran

    发明人： Richard Guarino ,  Sharon Presnell ,  Andrea Liebmann-Vinson ,  Mohammad Heidaran

    IPC分类号： C12N5/071 ,  G01N33/50 ,  C12N5/08

    CPC分类号： G01N33/5067 ,  C12N5/067 ,  C12N5/0671 ,  C12N2533/32 ,  C12N2533/54

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.

    摘要翻译： 用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，层粘连蛋白，胶原I，胶原 III，胶原IV和胶原蛋白VI。 此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL）， 聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。 该表面用于细胞培养以促进原代肝细胞的细胞附着，存活和/或增殖。 本发明还涉及利用该表面的方法，例如细胞附着，存活和/或增殖的方法。 还公开了使用无血清培养基的细胞培养物中的表面。 还公开了使用本发明的表面进行筛选的方法。

    PHARMACEUTICAL COMPOSITIONS FOR TREATING OR PREVENTING MIGAINES
    9.
    发明申请
    PHARMACEUTICAL COMPOSITIONS FOR TREATING OR PREVENTING MIGAINES 审中-公开
    用于治疗或预防移植的药物组合物

    公开(公告)号：US20100273844A1

    公开(公告)日：2010-10-28

    申请号：US11992714

    申请日：2006-09-27

    申请人： Richard Guarino ,  Eyal S. Ron

    发明人： Richard Guarino ,  Eyal S. Ron

    IPC分类号： A61K31/42 ,  C07D261/18 ,  A61P25/06

    CPC分类号： A61K31/137

    摘要： The present invention provides a method for treating or preventing one or more types of migraines by administering a monoamine oxidase inhibitor, preferably isocarboxazid, in a rapid-release formulation. The present invention also provides a pharmaceutical composition suitable for treating or preventing migraines and having at least one monoamine oxidase inhibitor.

    摘要翻译： 本发明提供了通过在快速释放制剂中施用单胺氧化酶抑制剂（优选异卡波肼）来治疗或预防一种或多种类型的偏头痛的方法。 本发明还提供了适用于治疗或预防偏头痛并具有至少一种单胺氧化酶抑制剂的药物组合物。