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139 条记录 (耗时 0.035 秒)

    Methods for high fidelity production of long nucleic acid molecules
    12.
    发明授权
    Methods for high fidelity production of long nucleic acid molecules 失效
    高保真生产长核酸分子的方法

    公开(公告)号：US08206952B2

    公开(公告)日：2012-06-26

    申请号：US13019092

    申请日：2011-02-01

    申请人： Peter A. Carr ,  Brian Y. Chow ,  Joseph M. Jacobson ,  David W. Mosley ,  Christopher Emig

    发明人： Peter A. Carr ,  Brian Y. Chow ,  Joseph M. Jacobson ,  David W. Mosley ,  Christopher Emig

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12P19/34 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6848 ,  C12Q2565/518 ,  C12Q2565/513 ,  C12Q2521/514

    摘要： In a method for synthesizing a long nucleic acid molecule, a first immobilized nucleic acid has a first 5′ region and a first 3′ region and a second immobilized nucleic acid has a second 5′ region and a second 3′ region. The second 3′ region and the first 5′ region have identical nucleic acid sequences. An oligonucleotide is hybridized to the first 3′ region, extending the hybridized oligonucleotide and producing a first extension product having a 3′ region that is complementary to the first 5′ region. The 3′ region of the first extension product is hybridized to the second 3′ region, extending the 3′ region of the first extension product and producing a synthesized nucleic acid molecule having a 3′ region that is complementary to the second 5′ region, wherein the synthesized nucleic acid molecule has a sequence complementary to the first and second 3′ and 5′ regions.

    摘要翻译： 在合成长核酸分子的方法中，第一固定化核酸具有第一5'区和第3'区，第二固定化核具有第二5'区和第二3'区。 第二个3'区和第5个'区具有相同的核酸序列。 将寡核苷酸与前3'区域杂交，延伸杂交的寡核苷酸并产生具有与前5'区互补的3'区的第一延伸产物。 第一延伸产物的3'区域与第二3'区域杂交，延伸第一延伸产物的3'区域并产生具有与第二5'区域互补的3'区域的合成核酸分子， 其中所述合成的核酸分子具有与所述第一和第二3'和5'区互补的序列。

    Nanoparticle chains and preparation thereof
    16.
    发明授权
    Nanoparticle chains and preparation thereof 有权
    纳米粒子链及其制备

    公开(公告)号：US07476442B2

    公开(公告)日：2009-01-13

    申请号：US10621790

    申请日：2003-07-17

    申请人： Joseph M. Jacobson ,  David W. Mosley ,  Kie-Moon Sung

    发明人： Joseph M. Jacobson ,  David W. Mosley ,  Kie-Moon Sung

    IPC分类号： B32B5/16

    CPC分类号： C08B37/0012 ,  B05D1/00 ,  C01B13/00 ,  C08B37/0015 ,  Y10S977/778 ,  Y10S977/882 ,  Y10T428/25 ,  Y10T428/2991

    摘要： Fabrication and arrangement of nanoparticles into one-dimensional linear chains is achieved by successive chemical reactions, each reaction adding one or more nanoparticles by building onto exposed, unprotected linker functionalities. Optionally, protecting groups may be used to control and organize growth. Nanoparticle spheres are functionalized in a controlled manner in order to enable covalent linkages. Functionalization of nanoparticles is accomplished by either ligand exchange or chemical modification of the terminal functional groups of the capping ligand. Nanoparticle chains are obtained by a variety of connectivity modes such as direct coupling, use of linker molecules, and use of linear polymeric templates. In particular, a versatile building block system is obtained through controlled monofunctionalization of nanoparticles.

    摘要翻译： 通过连续的化学反应来实现纳米颗粒到一维线性链中的制备和排列，每个反应通过构建到暴露的未受保护的接头功能上而添加一种或多种纳米颗粒。 任选地，保护基团可用于控制和组织生长。 纳米颗粒球以受控的方式进行功能化，以实现共价键。 纳米颗粒的官能化通过配体交换或封端配体的末端官能团的化学修饰来实现。 通过各种连接模式（例如直接偶联，使用连接分子）和使用线性聚合物模板获得纳米颗粒链。 特别地，通过纳米颗粒的受控单官能化获得通用的结构单元体系。

    ELECTROSTATICALLY ADDRESSABLE ELECTROPHORETIC DISPLAY
    17.
    发明申请
    ELECTROSTATICALLY ADDRESSABLE ELECTROPHORETIC DISPLAY 失效
    静电地址电泳显示器

    公开(公告)号：US20080150888A1

    公开(公告)日：2008-06-26

    申请号：US11970811

    申请日：2008-01-08

    申请人： Jonathan D. Albert ,  Barrett Comiskey ,  Joseph M. Jacobson

    发明人： Jonathan D. Albert ,  Barrett Comiskey ,  Joseph M. Jacobson

    IPC分类号： G09G3/34

    CPC分类号： G09G3/344 ,  G06F3/03545 ,  G09G3/3446 ,  G09G2300/0434

    摘要： Novel addressing schemes for controlling electronically addressable displays include a scheme for rear-addressing displays, which allows for in-plane switching of the display material. Other schemes include a rear-addressing scheme which uses a retroreflecting surface to enable greater viewing angle and contrast. Another scheme includes an electrode structure that facilitates manufacture and control of a color display. Another electrode structure facilitates addressing a display using an electrostatic stylus. Methods of using the disclosed electrode structures are also disclosed. Another scheme includes devices combining display materials with silicon transistor addressing structures.

    摘要翻译： 用于控制电子可寻址显示器的新型寻址方案包括用于后寻址显示器的方案，其允许显示材料的平面内切换。 其他方案包括使用回射表面以实现更大视角和对比度的后方寻址方案。 另一方案包括有利于彩色显示器的制造和控制的电极结构。 另一种电极结构有助于使用静电触针来寻址显示器。 还公开了使用所公开的电极结构的方法。 另一种方案包括将显示材料与硅晶体管寻址结构组合在一起的装置。

    Templated monolayer polymerization and replication
    18.
    发明授权
    Templated monolayer polymerization and replication 失效
    模板单层聚合和复制

    公开(公告)号：US07311943B2

    公开(公告)日：2007-12-25

    申请号：US10621897

    申请日：2003-07-17

    申请人： Joseph M. Jacobson ,  David W. Mosley

    发明人： Joseph M. Jacobson ,  David W. Mosley

    IPC分类号： B05B3/00

    CPC分类号： H01L21/02112 ,  B05D1/185 ,  B82Y30/00 ,  B82Y40/00 ,  G01N31/22 ,  G03F7/165 ,  H01L21/283 ,  Y10S977/882 ,  Y10S977/883

    摘要： A self-replicating monolayer system employing polymerization of monomers or nanoparticle ensembles on a defined template provides a method for synthesis of two-dimensional single molecule polymers. Systems of self-replicating monolayers may be used as templates for the growth of inorganic colloids. A preferred embodiment is a SAM-based replication, wherein an initial monolayer is patterned and used as a template for self-assembly of a second monolayer by molecular recognition. Once the second monolayer has formed, it is polymerized in place and the two monolayers are separated to form a replicate. Both monolayers may then function as templates for monolayer assemblies. A generic self-replicating monomer unit suitable for use in one embodiment comprises a polymerizable moiety attached by methylene repeats to a recognition element and an ending unit that will not interfere with the chosen recognition chemistry. The recognition element is self-complementary, unless a set of two replicating monomers with compatible cross-linking chemistry is employed. In a two-component replication system utilizing two different kinds of recognition chemistries, the initial template undergoes replication cycles, while maintaining the two-dimensional segregation of the two types of monomers. During subsequent replications, the component domains experience little or no mixing, allowing the two-component, patterned assembly to be exponentially replicated. After replication, selective mineralization and/or electroless plating may produce a two-dimensional inorganic sheet having patterned domains within it.

    摘要翻译： 使用单体或纳米颗粒聚合体在定义的模板上进行聚合的自复制单层体系提供了合成二维单分子聚合物的方法。 自复制单层的系统可以用作无机胶体生长的模板。 优选的实施方案是基于SAM的复制，其中将初始单层图案化并用作通过分子识别自组装第二单层的模板。 一旦形成了第二个单层，就将其聚合，将两个单层分离形成一个复制体。 然后两个单层可以用作单层组件的模板。 适用于一个实施方案的通用自复制单体单元包括通过亚甲基重复序列连接到识别元件的可聚合部分和不干扰所选择的识别化学的结束单元。 识别元件是自互补的，除非使用具有相容的交联化学的两组复制单体。 在使用两种不同类型的识别化学物质的双组分复制系统中，初始模板经历复制循环，同时保持两种类型的单体的二维分离。 在随后的复制过程中，组件域经历很少或不混合，允许双组分图案化组件以指数方式复制。 复制后，选择性矿化和/或化学镀可以产生其内具有图案区的二维无机片。