公开(公告)号：US06982144B2

公开(公告)日：2006-01-03

申请号：US09972834

申请日：2001-10-04

申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

IPC分类号： C12Q1/68 ,  C12N9/10 ,  C07K1/00

CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

公开(公告)号：US06739676B1

公开(公告)日：2004-05-25

申请号：US09233583

申请日：1999-01-19

申请人： Hiroshi Isono ,  Yasuji Mizutani ,  Yutaka Onuma ,  Koichi Kondo ,  Hirofumi Nitta ,  Motoshi Suzuki

发明人： Hiroshi Isono ,  Yasuji Mizutani ,  Yutaka Onuma ,  Koichi Kondo ,  Hirofumi Nitta ,  Motoshi Suzuki

IPC分类号： B60T844

CPC分类号： B60T8/442 ,  B60T8/365 ,  B60T8/4872 ,  B60T13/575 ,  B60T13/686

摘要： Vehicle braking system including a vacuum booster for transmitting a boosted brake operating force to a master cylinder such that the boosting ratio is reduced at a fixed transition point before the boosting limit point is reached, a brake having a wheel brake cylinder connected through a fluid passage to a master cylinder serving as a first hydraulic pressure source, for braking a wheel, and a pressure increasing device having a second hydraulic pressure source connected to the fluid passage, and wherein the pressure increasing device initiating a pressure increasing operation, when the brake operating force has increased to the transition point, to increase the wheel cylinder pressure to be higher than the master cylinder pressure, by using the second hydraulic pressure source.

摘要翻译： 车辆制动系统包括用于将升压的制动器操作力传递到主缸的真空助力器，使得在升压极限点之前的固定转变点处升压比减小;制动器，其具有通过流体通道连接的车轮制动缸 到用于制动车轮的第一液压源的主缸，以及具有连接到流体通道的第二液压源的增压装置，并且其中，当制动器操作时，增压装置启动增压操作 力已经增加到过渡点，通过使用第二液压源将轮缸压力增加到高于主缸压力。

公开(公告)号：US06276763B1

公开(公告)日：2001-08-21

申请号：US09248854

申请日：1999-02-11

申请人： Hiroshi Isono ,  Motoshi Suzuki ,  Hirofumi Nitta

发明人： Hiroshi Isono ,  Motoshi Suzuki ,  Hirofumi Nitta

IPC分类号： B60T832

CPC分类号： B60T8/442 ,  B60T7/042 ,  B60T8/94 ,  B60T13/686 ,  B60T13/72 ,  B60T17/221

摘要： Method of diagnosing a booster of a braking system including a brake operating member operated with a brake operating force, a master cylinder for producing a hydraulic pressure on the basis of an input force received from the booster as a result of boosting of the brake operating force by said booster, and a wheel brake cylinder which is activated by the hydraulic pressure produced by the master cylinder, to brake a vehicle wheel, wherein the booster is determined to be abnormal if the detected input and output of the booster do not meet a predetermined nominal relationship therebetween. Also disclosed is a diagnosing device capable of practicing the method.

摘要翻译： 诊断包括由制动器操作力操作的制动操作构件的制动系统的助力器的方法，用于根据从助力器接收的输入力产生液压的主缸，其是由于升高制动器操作力 通过所述助力器和由主缸产生的液压激活的车轮制动缸，以制动车轮，其中如果检测到的增压器的输入和输出不符合预定的，预定的增压器被确定为异常 名义上的关系。 还公开了能够实施该方法的诊断装置。

公开(公告)号：US5004264A

公开(公告)日：1991-04-02

申请号：US386041

申请日：1989-07-27

申请人： Tetsuji Kozaki ,  Hiroshi Ishikawa ,  Hideaki Yasui ,  Mamoru Shimamoto ,  Katsuhiko Hibino ,  Motoshi Suzuki

发明人： Tetsuji Kozaki ,  Hiroshi Ishikawa ,  Hideaki Yasui ,  Mamoru Shimamoto ,  Katsuhiko Hibino ,  Motoshi Suzuki

IPC分类号： B60G17/018 ,  B60G17/019 ,  B60G21/055 ,  G05D1/08

CPC分类号： G05D1/0891 ,  B60G17/018 ,  B60G17/019 ,  B60G21/0555 ,  B60G2202/135 ,  B60G2202/413 ,  B60G2400/204 ,  B60G2400/412 ,  B60G2600/16 ,  B60G2600/22 ,  B60G2800/012

摘要： A position control device includes a fluid-operated directional control valve for controlling the position of the piston of a cylinder assembly under fluid pressure in response to a control signal from an electronic controller. The position of the piston is predicted based on the control signal. A position detector detects when the piston has actually reached a predetermined position. When it is detected that the piston has actually reached the predetermined position, the predicted position of the piston is corrected. The electronic controller applies a control signal to the directional control valve so that the predicted position as corrected will be equalized to a target position for the piston. The predetermined position is a substantially intermediate position of the stroke of the piston. The directional control valve comprises a solenoid-controlled valve for controlling the flow rate of the fluid depending on the magnitude of a current supplied to the solenoid-operated valve. The position of the piston is calculated based on the magnitude of the current and a period of time during which the current is supplied to the solenoid-operated valve. An automotive suspension system incorporating the position control device is also disclosed.

摘要翻译： 位置控制装置包括流体操作的方向控制阀，用于响应于来自电子控制器的控制信号，在液压下控制气缸组件的活塞的位置。 基于控制信号预测活塞的位置。 位置检测器检测活塞何时已经实际到达预定位置。 当检测到活塞实际上达到预定位置时，校正活塞的预测位置。 电子控制器向方向控制阀施加控制信号，使得校正的预测位置将与活塞的目标位置相等。 预定位置是活塞行程的大致中间位置。 方向控制阀包括用于根据提供给电磁阀的电流的大小来控制流体的流量的电磁阀。 活塞的位置基于电流的大小和向电磁阀提供电流的时间段来计算。 还公开了一种结合了位置控制装置的汽车悬架系统。

公开(公告)号：US08089950B2

公开(公告)日：2012-01-03

申请号：US11571892

申请日：2005-07-13

申请人： Motoshi Suzuki ,  Shohei Taniguchi

发明人： Motoshi Suzuki ,  Shohei Taniguchi

IPC分类号： H04J3/06

CPC分类号： H04L7/0054 ,  H04W52/245 ,  H04W56/00 ,  H04W88/02

摘要： A mobile station 200 includes a receiving field level detecting unit 213 to convert a level of a wireless signal into an RSSI output, and a wireless frame timing deciding unit 214 to decide a wireless frame timing from the transient characteristic of the RSSI output. By deciding the wireless frame timing from the RSSI output, synchronous establishment can be realized even when the mobile station 200 does not include a demodulation circuit. The mobile station can perform the synchronous establishment without being increased in size.

摘要翻译： 移动站200包括：接收场电平检测单元213，用于将无线信号的电平转换为RSSI输出;以及无线帧定时判定单元214，用于根据RSSI输出的瞬态特性来确定无线帧定时。 通过从RSSI输出决定无线帧定时，即使移动台200不包括解调电路，也可以实现同步建立。 移动台可以在不增加大小的情况下执行同步建立。

公开(公告)号：US20100249058A1

公开(公告)日：2010-09-30

申请号：US12444034

申请日：2007-10-11

申请人： Shinji Ito ,  Motoshi Suzuki ,  Kuniko Suzuki ,  Yasuo Kobayashi

发明人： Shinji Ito ,  Motoshi Suzuki ,  Kuniko Suzuki ,  Yasuo Kobayashi

IPC分类号： A61K31/70 ,  C07H13/02 ,  A61K31/7016 ,  A61P31/04

CPC分类号： A61K31/7028 ,  A23K20/10 ,  A23K20/158 ,  A23K20/163 ,  A23K50/10 ,  A23K50/30 ,  A23K50/70 ,  A23K50/75 ,  A61K31/7032 ,  A61K35/742 ,  A61K35/744 ,  A61K36/06

摘要： To provide safe and easy means for preventing or treating diseases of birds and mammals, in particular, livestock. In particular, to provide means for preventing or treating an infectious disease caused by a Gram-positive bacterium. In addition, to improve fermentation in the rumen of a ruminant animal, to contribute to suppression of the generation of greenhouse gas, and to increase the feed efficiency. Mannosylerythritol lipids (MEL) and/or rhamnolipids are given to birds or mammals.

摘要翻译： 为预防或治疗鸟类和哺乳动物，特别是牲畜的疾病提供安全和方便的手段。 特别是提供预防或治疗由革兰氏阳性菌引起的传染病的方法。 此外，为了改善反刍动物的瘤胃发酵，有助于抑制温室气体的产生，并提高饲料效率。 对于鸟类或哺乳动物，赋予甘露糖醇脂质（MEL）和/或鼠李糖脂。

公开(公告)号：US20080056166A1

公开(公告)日：2008-03-06

申请号：US11571892

申请日：2005-07-13

申请人： Motoshi Suzuki ,  Shohei Taniguchi

发明人： Motoshi Suzuki ,  Shohei Taniguchi

IPC分类号： H04J3/00

CPC分类号： H04L7/0054 ,  H04W52/245 ,  H04W56/00 ,  H04W88/02

摘要： A mobile station 200 includes a receiving field level detecting unit 213 to convert a level of a wireless signal into an RSSI output, and a wireless frame timing deciding unit 214 to decide a wireless frame timing from the transient characteristic of the RSSI output. By deciding the wireless frame timing from the RSSI output, synchronous establishment can be realized even when the mobile station 200 does not include a demodulation circuit. The mobile station can perform the synchronous establishment without being increased in size.

摘要翻译： 移动站200包括：将无线信号的电平转换为RSSI输出的接收场电平检测单元213，以及根据RSSI输出的瞬态特性来决定无线帧定时的无线帧定时判定单元214。 通过从RSSI输出决定无线帧定时，即使移动台200不包括解调电路，也可以实现同步建立。 移动台可以在不增加大小的情况下执行同步建立。

公开(公告)号：US07335310B2

公开(公告)日：2008-02-26

申请号：US10544074

申请日：2003-08-04

申请人： Motoshi Suzuki ,  Takahiro Kawabata ,  Hideo Miyamoto ,  Takahisa Muramoto ,  Masashi Machida ,  Shingo Ogoshi

发明人： Motoshi Suzuki ,  Takahiro Kawabata ,  Hideo Miyamoto ,  Takahisa Muramoto ,  Masashi Machida ,  Shingo Ogoshi

IPC分类号： C02F3/00

CPC分类号： C02F1/56 ,  C02F1/28 ,  C02F1/281 ,  C02F1/283 ,  C02F1/286 ,  C02F1/44 ,  C02F1/52 ,  C02F1/5245 ,  C02F1/72 ,  C02F1/722 ,  C02F9/00 ,  C02F2101/301 ,  C02F2101/305 ,  C02F2101/32 ,  C02F2101/327 ,  C02F2101/36 ,  C02F2101/366

摘要： A method of treating hardly decomposable harmful substance comprising (A) a step for adding coagulant into wastewater containing a hardly decomposable harmful substance, (B) a step for performing solid-liquid separation which separates a solid containing the hardly decomposable harmful substance from a liquid, and (C) a step for decomposing the hardly decomposable harmful substance by bringing peroxide into contact with the separated solid. Efficiently and safely treating the hardly decomposable harmful substances by means of low-cost facilities realizes.

摘要翻译： 一种处理难分解性有害物质的方法，包括（A）将凝结剂添加到含有难分解性有害物质的废水中的工序，（B）进行固液分离的步骤，将含有难分解性有害物质的固体与液体分离 ，（C）通过使过氧化物与分离后的固体接触来分解难分解性有害物质的步骤。 通过低成本设施有效安全地处理难分解的有害物质。

公开(公告)号：US07151910B2

公开(公告)日：2006-12-19

申请号：US10452182

申请日：2003-06-02

申请人： Motoshi Suzuki ,  Hiroshi Yamamoto

发明人： Motoshi Suzuki ,  Hiroshi Yamamoto

IPC分类号： H04B7/00

CPC分类号： H04R1/083 ,  H04R3/00 ,  H04R29/004 ,  H04R2420/07

摘要： The wireless microphone information displaying apparatus comprise a plurality of wireless microphones having information respectively assigned thereto, a wireless microphone supporting device having a plurality of supporting portion for supporting the microphones, respectively, and a plurality of connecting devices for electrically connecting the microphones with the unit. Each of the microphones contains a wireless microphone control unit. The supporting device comprises a unit control unit, a display panel for displaying the information assigned to the microphones. The microphone information is displayed on the display panel, when the microphones are supported o the supporting device.

公开(公告)号：US20050196800A1

公开(公告)日：2005-09-08

申请号：US11096645

申请日：2005-03-31

申请人： Lawrence Loeb ,  Leroy Hood ,  Motoshi Suzuki

发明人： Lawrence Loeb ,  Leroy Hood ,  Motoshi Suzuki

IPC分类号： C12N9/10 ,  C12N9/12 ,  C12N9/22 ,  C12P19/34 ,  C12Q1/34 ,  C12Q1/68

CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。