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公开(公告)号:US5667990A
公开(公告)日:1997-09-16
申请号:US458023
申请日:1995-06-01
IPC分类号: C12N15/09 , C12N1/15 , C12N9/00 , C12N9/08 , C12N9/20 , C12N9/30 , C12N15/80 , C12P21/02 , C12R1/66 , C07H21/04 , C12N15/11
CPC分类号: C12N9/0065 , C12N15/80 , C12N9/20 , C12N9/242
摘要: The present invention relates to a novel expression system in which A. japonicus-type species are used as host cells for expression of heterologous proteins.
摘要翻译: 本发明涉及一种新型的表达系统,其中日本粳稻型物种用作表达异源蛋白质的宿主细胞。
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公开(公告)号:US09222095B2
公开(公告)日:2015-12-29
申请号:US13704856
申请日:2011-06-23
申请人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi
发明人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi
IPC分类号: C12N15/80 , C07K14/195
CPC分类号: C12N15/80 , C07K14/195
摘要: The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.
摘要翻译: 本发明涉及具有前导序列功能的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体,载体和宿主细胞以及使用多核苷酸产生多肽的方法。
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公开(公告)号:US20140017749A1
公开(公告)日:2014-01-16
申请号:US13994310
申请日:2011-12-21
申请人: Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
发明人: Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
IPC分类号: C12P7/06
CPC分类号: C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , C12Y302/01003 , C12Y304/24 , Y02E50/17
摘要: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
摘要翻译: 本发明涉及用于从含淀粉材料生产发酵产物的方法,其中在液化期间存在和/或加入热稳定性α-淀粉酶和任选的热稳定蛋白酶。 本发明还涉及适用于本发明方法的组合物。
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公开(公告)号:US08263376B2
公开(公告)日:2012-09-11
申请号:US13076803
申请日:2011-03-31
申请人: Akiko Sugio , Shinobu Takagi
发明人: Akiko Sugio , Shinobu Takagi
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The present disclosure relates to an isolated lipoxygenase. The lipoxygenase of the present disclosure includes polypeptide with at least 80% sequence homology to the mature peptide shown in SEQ ID NO: 2. Lipoxygenase of the present disclosure is suitable for use in, among other things, baking and in detergent compositions.
摘要翻译: 本公开涉及分离的脂氧合酶。 本公开的脂氧合酶包括与SEQ ID NO:2所示的成熟肽具有至少80%的序列同源性的多肽。本公开的脂氧合酶适合用于烘烤和洗涤剂组合物中。
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公开(公告)号:US07883883B2
公开(公告)日:2011-02-08
申请号:US10877849
申请日:2004-06-25
申请人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi , Carsten Hjort , Anders Vikso-Nielsen , Eric Allain , Shiro Fukuyama , Tomoko Matsui
发明人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi , Carsten Hjort , Anders Vikso-Nielsen , Eric Allain , Shiro Fukuyama , Tomoko Matsui
CPC分类号: C12P19/14 , C07K2319/00 , C12N9/2408 , C12N9/2411 , C12N9/2414 , C12N9/242 , C12N9/2425 , C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/16 , C12P19/22 , C13K11/00 , Y02E50/17
摘要: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.
摘要翻译: 本发明涉及包含碳水化合物结合模块氨基酸序列和真菌α-淀粉酶氨基酸序列以及包含碳水化合物结合模块和α-淀粉酶催化模块的真菌野生型酶的变体的杂合酶。 本发明还涉及混合酶或变体在淀粉液化中的用途。
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公开(公告)号:US20070292581A1
公开(公告)日:2007-12-20
申请号:US11832472
申请日:2007-08-01
申请人: Akiko Sugio , Shinobu Takagi
发明人: Akiko Sugio , Shinobu Takagi
IPC分类号: A23L1/277 , A21D2/08 , C07H21/04 , C09B67/00 , C12N1/15 , C12N15/00 , C12N9/02 , C12P21/00 , C12P7/64
CPC分类号: C12P7/6427 , A21D8/042 , A23L33/195 , C11D3/386 , C12N9/0069 , C12Y113/11012
摘要: The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.
摘要翻译: 本发明人已经发现了一种来自Manaporthe salvinii的新型真菌脂氧合酶并确定其序列。 他们对基因进行了测序,并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。
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公开(公告)号:US20050054071A1
公开(公告)日:2005-03-10
申请号:US10877849
申请日:2004-06-25
申请人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi , Eric Allain , Carsten Hjort , Anders Vikso-Nielsen
发明人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi , Eric Allain , Carsten Hjort , Anders Vikso-Nielsen
CPC分类号: C12P19/14 , C07K2319/00 , C12N9/2408 , C12N9/2411 , C12N9/2414 , C12N9/242 , C12N9/2425 , C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/16 , C12P19/22 , C13K11/00 , Y02E50/17
摘要: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.
摘要翻译: 本发明涉及包含碳水化合物结合模块氨基酸序列和真菌α-淀粉酶氨基酸序列以及包含碳水化合物结合模块和α-淀粉酶催化模块的真菌野生型酶的变体的杂合酶。 本发明还涉及混合酶或变体在淀粉液化中的用途。
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公开(公告)号:US6001639A
公开(公告)日:1999-12-14
申请号:US651136
申请日:1996-05-21
申请人: Martin Schulein , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Iium Nielsen , Michiko Ihara , Shinobu Takagi
发明人: Martin Schulein , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Iium Nielsen , Michiko Ihara , Shinobu Takagi
IPC分类号: C12N15/09 , C05F11/08 , C05F17/00 , C07G99/00 , C11D3/386 , C12N1/19 , C12N9/42 , C12N15/00 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/80 , C12S3/04 , C12S11/00 , D06M13/02 , D06M13/322 , D06M13/46 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21H11/16 , D21H11/20 , D21H17/00 , D21H21/10 , D21C1/00
CPC分类号: C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H21/10 , C07K2319/00 , D21H17/005 , Y02P20/145 , Y02W30/43
摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译: 本发明涉及基本上由具有纤维素分解活性的酶组成的酶制剂,其包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列:Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa(SEQ ID NO:79) - 1 2 3 4 5 6 7 8 9 - - Xaa Cys Xaa Trp Xaa - 10 11 12 13 14 - 和由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列--Trp Cys Cys Xaa Cys(SEQ ID NO :80) - 1 2 3 4 5 - 其中,在第一序列的位置3,氨基酸是Trp,Tyr或Phe; 在第一序列的位置4,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 分别位于第一序列的第9,10,12和14位,在第二序列的位置4中,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当位置12的氨基残基为Ser时,14位的氨基酸残基不为Ser,(ii)当位置12的氨基残基为Gly时,位置14的氨基酸残基为 Ala在工业应用中表现非常好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。
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公开(公告)号:US09816112B2
公开(公告)日:2017-11-14
申请号:US13994310
申请日:2011-12-21
申请人: Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
发明人: Randy Deinhammer , Suzanne Clark , Mauricio Quiros , John Matthews , Anne Glud Hjulmand , Chee Leong Soong , Tomoko Matsui , Shinobu Takagi
CPC分类号: C12P7/06 , C12P19/02 , C12P19/14 , C12P19/20 , C12Y302/01001 , C12Y302/01003 , C12Y304/24 , Y02E50/17
摘要: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
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公开(公告)号:US20130095525A1
公开(公告)日:2013-04-18
申请号:US13704856
申请日:2011-06-23
申请人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi
发明人: Hiroaki Udagawa , Rikako Taira , Shinobu Takagi
IPC分类号: C12N15/80
CPC分类号: C12N15/80 , C07K14/195
摘要: The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.
摘要翻译: 本发明涉及具有前导序列功能的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体,载体和宿主细胞以及使用多核苷酸产生多肽的方法。
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