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80 条记录 (耗时 0.046 秒)

    Unsymmetrical cyanine dimer compounds and their application
    15.
    发明授权
    Unsymmetrical cyanine dimer compounds and their application 有权
    不对称花青二聚体化合物及其应用

    公开(公告)号：US07888136B2

    公开(公告)日：2011-02-15

    申请号：US12099020

    申请日：2008-04-07

    申请人： Stephen Yue ,  Ching-Ying Cheung

    发明人： Stephen Yue ,  Ching-Ying Cheung

    IPC分类号： G01N33/53 ,  B01L3/00 ,  C12Q1/68 ,  C07H19/04

    CPC分类号： C12Q1/6816 ,  Y10S436/80 ,  C12Q2563/173

    摘要： Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA.

    摘要翻译： 本发明的实施方案提供了用于检测样品中核酸的方法和核酸报告分子。 核酸报告分子包含两个不对称的花青单体部分，其可以相同或不同，其通过包含至少一个芳族，杂芳族，环状或杂环部分的连接体共价连接，该部分包含3-20个非氢原子，所述非氢原子选自 由O，N，S，P和C组成的组。接头可以是刚性的，相对柔性的或其某种程度的。 不对称花青单体部分包含取代或未取代的苯并唑鎓部分和通过单甲基，三甲基或五甲氧基的次甲基桥连接的取代或未取代的吡啶鎓或喹啉鎓部分。 连接体通过连接到单体部分的吡啶鎓或喹啉鎓部分而形成花青二聚体化合物。 本发明的核酸报告分子可用于形成核酸 - 报道分子复合物并检测核酸。 特别地，具有刚性连接体和具有单甲基桥的单体部分的本发明的核酸报告分子在DNA存在下可用于检测RNA。

    Apparatus and methods for self-biasing differential signaling circuitry having multimode output configurations for low voltage applications
    16.
    发明授权
    Apparatus and methods for self-biasing differential signaling circuitry having multimode output configurations for low voltage applications 有权
    具有用于低电压应用的多模输出配置的用于自偏压差动信号电路的装置和方法

    公开(公告)号：US07724037B2

    公开(公告)日：2010-05-25

    申请号：US12352916

    申请日：2009-01-13

    申请人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard W. Fung

    发明人： Junho Cho ,  Nancy Chan ,  Ramesh Senthinathan ,  Stephen Yue ,  Richard W. Fung

    IPC分类号： H03K19/094

    CPC分类号： H03K17/302 ,  H03K17/04106

    摘要： The present disclosure relates to a differential signaling circuit including differential signaling circuitry having at least one output and one input, that can operate in multiple mode of operations while using a single, low voltage supply source. Two or more switches are included and configured to selectively couple a supply voltage to the output dependent on a mode of operation of the differential signaling circuitry. The circuit also includes a switch control biasing circuit operatively coupled to at least one of the switches and to the output of the differential signaling circuitry. The switch control biasing circuit provides a switch control biasing voltage to control a state of the switch based on a voltage level of the output. Further, a bulk biasing circuit is included and operatively coupled to the switch. The bulk biasing circuit selectively provides a bulk biasing voltage to the switch based on the voltage level of the output.

    摘要翻译： 本公开涉及一种差分信号电路，其包括具有至少一个输出和一个输入的差分信号电路，其可以在使用单个低电压电源时在多种操作模式下操作。 两个或更多个开关被包括并配置成根据差分信号电路的操作模式选择性地将电源电压耦合到输出。 电路还包括可操作地耦合到至少一个开关和差分信号电路的输出的开关控制偏置电路。 开关控制偏置电路提供开关控制偏置电压，以基于输出的电压电平来控制开关的状态。 此外，体积偏置电路被包括并且可操作地耦合到开关。 体积偏置电路基于输出的电压电平选择性地向开关提供体积偏置电压。

    CYANINE COMPOUNDS AND THEIR APPLICATION AS QUENCHING COMPOUNDS
    18.
    发明申请
    CYANINE COMPOUNDS AND THEIR APPLICATION AS QUENCHING COMPOUNDS 审中-公开
    氰胺化合物及其作为化学化合物的应用

    公开(公告)号：US20080039630A1

    公开(公告)日：2008-02-14

    申请号：US11782244

    申请日：2007-07-24

    申请人： Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    发明人： Rosaria Haugland ,  Ching-Ying Cheung ,  Stephen Yue

    IPC分类号： C07D401/10 ,  C07D209/18 ,  C07D403/10 ,  C07D403/14 ,  C07D417/10

    CPC分类号： C12Q1/6876 ,  C07D209/12 ,  C07D209/14 ,  C07D209/24 ,  C07D401/04 ,  C09B23/12

    摘要： The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

    摘要翻译： 本发明提供了具有以下通式的方法和非荧光碳花青猝灭剂：其中A部分是取代的吡啶鎓，未取代的吡啶鎓，取代的喹啉鎓，未取代的喹啉鎓，取代的苯并唑鎓，未取代的苯并咪唑鎓，取代的吲哚鎓或取代的吲哚啉鎓。 本发明还提供了发光供体分子猝灭剂对和发光供体分子 - 猝灭剂 - 发光受体分子缀合物，其中猝灭剂是本发明的花青化合物。 能量转移对用于检测样品中感兴趣的分析物。