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29 条记录 (耗时 0.048 秒)

    External shutter for electrospray ionization mass spectrometry
    11.
    发明授权
    External shutter for electrospray ionization mass spectrometry 失效

    公开(公告)号：US06828550B2

    公开(公告)日：2004-12-07

    申请号：US10459791

    申请日：2003-06-12

    申请人： Richard Griffey ,  Steven Hofstadler

    发明人： Richard Griffey ,  Steven Hofstadler

    IPC分类号： B01D5944

    CPC分类号： H01J49/0077

    摘要： Novel methods and apparatuses for mass spectrometry are disclosed wherein a time slice of ions are selectively accumulated in an ion reservoir of a mass spectrometer and subsequently are allowed to undergo an ion-molecule reaction with a reactive species or are dissociated with coherent radiation prior to mass analysis. These methods and apparatuses are amenable to mass spectrometric analysis of singly or multiply charaged ions of peptides, proteins, carbohydrates, oligonucleotides, nucleic acids and small molecules as prepared by combinatorial or classical medicinal chemistry.

    Optimization of ligand affinity for RNA targets using mass spectrometry
    12.
    发明授权
    Optimization of ligand affinity for RNA targets using mass spectrometry 有权
    使用质谱法优化RNA靶标的配体亲和力

    公开(公告)号：US06770486B1

    公开(公告)日：2004-08-03

    申请号：US09499875

    申请日：2000-02-08

    申请人： Richard Griffey ,  Steven Hofstadler ,  Jared J. Drader ,  Kristin S. Lowery ,  Venkatraman Mohan

    发明人： Richard Griffey ,  Steven Hofstadler ,  Jared J. Drader ,  Kristin S. Lowery ,  Venkatraman Mohan

    IPC分类号： G01N2400

    CPC分类号： H01J49/0031 ,  H01J49/00 ,  Y10T436/24

    摘要： The present invention provides methods for the identification ligand compounds that bind to target molecules such as proteins or structured RNA with as little as millimolar (mM) affinity using mass spectrometry. The methods may be used to determine the mode of binding interaction between two or more of these ligand compounds to the target as well as their relative affinities. Also provided are methods for designing compounds having greater affinity to a target molecule by identifying two or more ligands using mass spectrometry methods of the invention and linking the ligands together to form a novel compound.

    摘要翻译： 本发明提供了使用质谱法鉴定与目标分子如蛋白质或结构化RNA结合至少达到毫摩尔（mM）亲和力的配体化合物的方法。 这些方法可用于确定两种或多种这些配体化合物与靶标之间的结合相互作用的模式以及它们的相对亲和力。 还提供了通过使用本发明的质谱方法鉴定两个或更多个配体并将配体连接在一起形成新化合物来设计对靶分子具有更高亲和力的化合物的方法。

    Mass spectrometric methods for biomolecular screening
    14.
    发明授权
    Mass spectrometric methods for biomolecular screening 有权
    用于生物分子筛选的质谱法

    公开(公告)号：US08211636B2

    公开(公告)日：2012-07-03

    申请号：US10608354

    申请日：2003-06-27

    申请人： Stanley T. Crook ,  Richard Griffey ,  Steven Hofstadler

    发明人： Stanley T. Crook ,  Richard Griffey ,  Steven Hofstadler

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  G01N24/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6811 ,  C40B40/06 ,  H01J49/004 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2525/101 ,  C12Q2565/133 ,  C12Q2525/121 ,  C12Q2565/607

    摘要： The present invention provides methods for the determination of the structure of biomolecular targets, as well as the site and nature of the interaction between ligands and biomolecular targets. The present invention also provides methods for the determination of the relative affinity of a ligand for the biomolecular target it interacts with. Also provided are methods for screening ligand or combinatorial libraries of compounds against one or more than one biological target molecules. The methods of the invention also allow determination of the relative binding affinity of combinatorial and other compounds for a biomolecular target. The present invention further provides methods for the use of mass modifying tags for screening multiple biomolecular targets. In a preferred embodiment, ligands which have great specificity and affinity for molecular interaction sites on biomolecules, especially RNA can be identified. In preferred embodiments, such identification can be made simultaneously with libraries of ligands.

    摘要翻译： 本发明提供了用于确定生物分子靶标的结构的方法，以及配体和生物分子靶标之间的相互作用的位点和性质。 本发明还提供了确定配体与其相互作用的生物分子靶的相对亲和力的方法。 还提供了用于筛选针对一种或多于一种生物靶分子的化合物的配体或组合文库的方法。 本发明的方法还允许确定组合和其它化合物对于生物分子靶标的相对结合亲和力。 本发明还提供使用质量修饰标签筛选多个生物分子靶标的方法。 在优选的实施方案中，可以鉴定对生物分子，特别是RNA上的分子相互作用位点具有极大特异性和亲和力的配体。 在优选的实施方案中，这种鉴定可以与配体文库同时进行。

    Optimization of ligand affinity for RNA targets using mass spectrometry
    15.
    发明授权
    Optimization of ligand affinity for RNA targets using mass spectrometry 有权
    使用质谱法优化RNA靶标的配体亲和力

    公开(公告)号：US08188011B1

    公开(公告)日：2012-05-29

    申请号：US09573479

    申请日：2000-05-16

    申请人： Richard Griffey ,  Steven Hofstadler ,  Jared J. Drader ,  Kristin S. Lowery

    发明人： Richard Griffey ,  Steven Hofstadler ,  Jared J. Drader ,  Kristin S. Lowery

    IPC分类号： C40B30/00

    CPC分类号： C12Q1/68 ,  C12Q2565/627 ,  Y10T436/24

    摘要： The present invention provides methods for the identification ligand compounds that bind to target molecules such as proteins or structured RNA with as little as millimolar (mM) affinity using mass spectrometry. The methods may be used to determine the mode of binding interaction between two or more of these ligand compounds to the target as well as their relative affinities. Also provided are methods for designing compounds having greater affinity to a target molecule by identifying two or more ligands using mass spectrometry methods of the invention and linking the ligands together to form a novel compound.

    摘要翻译： 本发明提供了使用质谱法鉴定与目标分子如蛋白质或结构化RNA结合至少达到毫摩尔（mM）亲和力的配体化合物的方法。 这些方法可用于确定两种或多种这些配体化合物与靶标之间的结合相互作用的模式以及它们的相对亲和力。 还提供了通过使用本发明的质谱方法鉴定两个或更多个配体并将配体连接在一起形成新化合物来设计对靶分子具有更高亲和力的化合物的方法。