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公开(公告)号：US20220249761A1
公开(公告)日：2022-08-11
申请号：US17617301
申请日：2020-06-23
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Jeremy A. Lombardo
Abstract: An integrated lipoaspirate processing system is disclosed for mechanically processing adipose tissue into a therapeutic material that, in some embodiments, may be directly injected into a subject. The system includes an emulsification device that is used to emulsify the lipoaspirate sample. The emulsified lipoaspirate is then filtered by a microfluidic filtration device. The filtrate of the microfluidic filtration device is then processed in a microfluidic dissociation device. The integrated platform or system helps standardize hydrodynamic processing of lipoaspirate by producing predictable and consistent shear forces and enabling automation in clinical settings. Progressive processing through multiple devices in series enables optimal recovery of regenerative cells while preventing clogging.
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公开(公告)号：US11130127B2
公开(公告)日：2021-09-28
申请号：US16777795
申请日：2020-01-30
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Ahmed Zobi , Justin Stovner , Hugo Salas , David Duarte , Jered Haun , Alan Widgerow , Derek Banyard
Abstract: Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).
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公开(公告)号：US10722540B1
公开(公告)日：2020-07-28
申请号：US15421206
申请日：2017-01-31
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Alan Widgerow , Derek Banyard , Xiaolong Qiu
Abstract: A method of processing a lipoaspirate sample includes mechanically processing the lipoaspirate sample to generate nanofat. The nanofat is then input into a microfluidic device comprising a plurality of serially arranged stages comprising one or more microfluidic channels having a plurality of expansion and constriction regions extending along the length of the one or more microfluidic channels, wherein each subsequent stage of the plurality has an increasing number of microfluidic channels of decreasing dimensions. The nanofat is flowed through the plurality of serially arranged stages in a plurality of cycles to generate sheared nanofat. The sheared nanofat is then collected after flowing through the plurality of serially arranged stages. The sheared nanofat may then be injected and/or applied to the subject. In an alternative embodiment, filtered or mechanically processed lipoaspirate may be run through the microfluidic device.
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公开(公告)号：US20170131187A1
公开(公告)日：2017-05-11
申请号：US15413201
申请日：2017-01-23
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Janice De Jesus , Elliot En-Yu Hui , Jiang Li
IPC: G01N1/28
Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
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公开(公告)号：US12201978B2
公开(公告)日：2025-01-21
申请号：US17412841
申请日：2021-08-26
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Ahmed Zobi , Justin Stovner , Hugo Salas , David Duarte , Jered Haun , Alan Widgerow , Derek Banyard
Abstract: Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).
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Patent Agency Ranking
16.发明公开公开(公告)号：US20230415157A1
公开(公告)日：2023-12-28
申请号：US18030959
申请日：2021-10-11
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Jeremy A. Lombardo
CPC classification number: B01L3/502753 , C12M23/16 , C12M45/09 , B01L2300/0816 , B01L2300/0864 , B01L2400/0622 , C12Y304/24007 , B01L2300/0681
Abstract: A microfluidic system for processing a tissue sample includes a microfluidic digestion device having an outlet fluidically connected to an inlet of a dissociation/filter device. The microfluidic digestion device includes an inlet and an outlet and a tissue chamber that connects to plurality of upstream fluidic channels and a plurality of downstream fluidic channels. The microfluidic dissociation/filter device includes an inlet, a first outlet, a second outlet, and a plurality of furcating dissociation channels having a plurality of expansion and constriction regions disposed along a length thereof, wherein one or more filters are disposed in a flow path downstream of the plurality of furcating dissociation channels. Pumps are provided to pump buffer and/or enzyme-containing fluid through the digestion device and dissociation/filter device. Tissue is initially processed in the digestion device and then passes into the dissociation/filter device.
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公开(公告)号：US20220097050A1
公开(公告)日：2022-03-31
申请号：US17412841
申请日：2021-08-26
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Ahmed Zobi , Justin Stovner , Hugo Salas , David Duarte , Jered Haun , Alan Widgerow , Derek Banyard
Abstract: Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).
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公开(公告)号：US20210205810A1
公开(公告)日：2021-07-08
申请号：US17058634
申请日：2019-05-29
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Marissa Noelani Pennell , Elliott E. Hui
Abstract: A microfluidic tissue dissociation and filtration device simultaneously filters large tissue fragments and dissociates smaller aggregates into single cells, thereby improving single cell yield and purity. The device includes an inlet coupled to a first microfluidic channel at an upstream location and a first outlet at a downstream location. A first filter membrane is interposed between the first microfluidic channel and a second microfluidic channel, wherein the second microfluidic channel is in fluidic communication with the first microfluidic channel via the first filter membrane. The first filter membrane operates under a tangential flow format. A second outlet is coupled to a downstream location of the second microfluidic channel and includes a second filter membrane interposed between the second outlet and the second microfluidic channel. The dual membrane device increased single cell numbers by at least 3-fold for different tissue types.
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公开(公告)号：US20210197191A1
公开(公告)日：2021-07-01
申请号：US17180711
申请日：2021-02-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
IPC: B01L3/00
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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公开(公告)号：US10589268B2
公开(公告)日：2020-03-17
申请号：US16101254
申请日：2018-08-10
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Ahmed Zobi , Justin Stovner , Hugo Salas , David Duarte , Jered Haun , Alan Widgerow , Derek Banyard
Abstract: Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).
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