公开(公告)号：US10697007B2

公开(公告)日：2020-06-30

申请号：US15317393

申请日：2015-06-25

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/70 ,  C12Q1/6886 ,  C12Q1/689

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20240042425A1

公开(公告)日：2024-02-08

申请号：US18027298

申请日：2021-09-28

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Jesse Qiuxu Zhang ,  Leqian Liu ,  Cyrille L. Delley ,  Russell Cole ,  Christian Siltanen

IPC: B01L3/02

CPC classification number: B01L3/0268 ,  B01L2400/0436 ,  B01L2400/0439

Abstract: Provided is a method of generating droplets that includes aspirating a first liquid into a tube, positioning the tube over a receiving liquid, and ejecting the first liquid to generate a plurality of droplets that contact the receiving liquid and remain discrete even after contacting the receiving liquid. Whereas many other droplet generators require complex microfluidics, the present methods allow generation of droplets without the need for microfluidics. The methods can be performed with existing commercially available macro-fluidic liquid handling devices. The methods can be used for digital PCR, digital MDA, enzyme screening, single cell analysis, and other applications involving droplets.

公开(公告)号：US11312990B2

公开(公告)日：2022-04-26

申请号：US16856343

申请日：2020-04-23

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/686 ,  C12Q1/70 ,  C12Q1/689 ,  C12Q1/6886

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20190330701A1

公开(公告)日：2019-10-31

申请号：US16382080

申请日：2019-04-11

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi

IPC: C12Q1/6886 ,  B01L7/00 ,  B01L3/00 ,  B01F13/00 ,  B01F5/06 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6844 ,  B01F3/08

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US10434507B2

公开(公告)日：2019-10-08

申请号：US15520056

申请日：2015-10-21

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Adam R. Sciambi ,  Russell Cole ,  Zev Jordan Gartner

IPC: B01L3/02 ,  G01N15/14 ,  B01L3/00 ,  G01N15/10 ,  G01N35/10

Abstract: Methods for delivering discrete entities including, e.g., cells, media or reagents to substrates are provided. In certain aspects, the methods include manipulating and/or analyzing qualities of the entities or biological components thereof. In some embodiments, the methods may be used to create arrays of microenvironments and/or for two and three-dimensional printing of tissues or structures. Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US20190218594A1

公开(公告)日：2019-07-18

申请号：US16324532

申请日：2017-08-09

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  David Sukovich

IPC: C12Q1/686 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6886

CPC classification number: C12Q1/686 ,  B01L3/502784 ,  B01L7/52 ,  C12M3/00 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2531/119 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The methods and systems described herein provide an improved emulsion droplet based nucleic acid amplification method, which allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., polymerase chain reaction (PCR). The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA.

公开(公告)号：US10161007B2

公开(公告)日：2018-12-25

申请号：US14420646

申请日：2013-08-12

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastbum ,  Adam R. Sciambi

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6844

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

公开(公告)号：US20170121756A1

公开(公告)日：2017-05-04

申请号：US15317393

申请日：2015-06-25

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Dennis Jay Eastburn ,  Adam R. Sciambi ,  Shaun Lim

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/70 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

公开(公告)号：US20170022538A1

公开(公告)日：2017-01-26

申请号：US15014976

申请日：2016-02-03

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  Shaun Lim ,  David Sukovich ,  Shea Thompson Lance

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01F3/0807 ,  B01F5/0653 ,  B01F13/0062 ,  C12Q1/68 ,  C40B40/06 ,  C40B50/06 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2565/607 ,  C12Q2565/626

Abstract: Multiple-emulsion nucleic acid amplification allows nucleic acids contained in biological systems to be detected, quantitated and/or sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the disclosure are also provided.

Abstract translation: 多重乳液核酸扩增允许根据用核酸扩增技术（例如PCR）检测的序列检测，定量和/或分选生物系统中所含的核酸。 核酸可以自由漂浮或包含在生物或非生物结构中，包括颗粒，病毒和细胞。 核酸可以包括例如DNA或RNA。 还提供了用于本公开的实践方法的系统和设备。

公开(公告)号：US20240150811A1

公开(公告)日：2024-05-09

申请号：US18212868

申请日：2023-06-22

Applicant: The Regents of the University of California

Inventor： Adam R. Abate ,  John R. Haliburton ,  Freeman Lan ,  Adam R. Sciambi

IPC: C12Q1/6804 ,  B01F25/433 ,  B01F33/302 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/6804 ,  B01F25/4338 ,  B01F33/3021 ,  B01L3/502784 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6874 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52

Abstract: Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.