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    METHODS AND KITS FOR EXTRACTION OF DNA
    271.
    发明申请
    METHODS AND KITS FOR EXTRACTION OF DNA 审中-公开

    公开(公告)号：US20180362962A1

    公开(公告)日：2018-12-20

    申请号：US16011597

    申请日：2018-06-18

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Maxim G. BREVNOV ,  Hemant S. PAWAR ,  Manohar R. FURTADO ,  Jaiprakash G. SHEWALE

    IPC: C12N15/10 ,  C12Q1/6806

    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

    System and method incorporating solid buffer
    279.
    发明授权
    System and method incorporating solid buffer 有权

    公开(公告)号：US10107825B2

    公开(公告)日：2018-10-23

    申请号：US14741084

    申请日：2015-06-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James A. Ball ,  Jonathan Schultz

    IPC: C12Q1/68 ,  G01N33/84 ,  C12Q1/6869 ,  B01J19/00

    Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

    Pinch flow regulator
    280.
    发明授权
    Pinch flow regulator 有权

    公开(公告)号：US10107403B2

    公开(公告)日：2018-10-23

    申请号：US15407333

    申请日：2017-01-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz

    IPC: B01L3/00 ,  F16K7/07 ,  G05D16/06

    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

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