公开(公告)号：US20190284548A1

公开(公告)日：2019-09-19

申请号：US16301176

申请日：2017-05-15

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Daniel ENDERLE ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N15/10 ,  C12N15/11

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US20190144941A1

公开(公告)日：2019-05-16

申请号：US16098500

申请日：2017-05-05

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital, Inc.

Inventor： Johan Karl Olov SKOG ,  Jamil AZZI

IPC: C12Q1/6876 ,  C12Q1/686

Abstract: The invention relates generally to the use of microvesicle RNA signatures for diagnosis, predicting, and/or to monitor treatment efficacy, including patients who are candidates for renal transplant and/or who have received a renal transplant.”

公开(公告)号：US20170211139A1

公开(公告)日：2017-07-27

申请号：US15325499

申请日：2015-07-13

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Mikkel Noerholm ,  Daniel Enderle

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6848 ,  C12Q2527/146 ,  C12Q2535/122 ,  C12Q2545/101

Abstract: The present invention relates to compositions that include one or more control molecules known as artificial reference sequences and methods of using these control molecules for estimating rare nucleic acid sequence variants from low copy numbers in ultra-deep sequencing.

公开(公告)号：US20160312211A1

公开(公告)日：2016-10-27

申请号：US14424694

申请日：2013-08-30

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel NOERHOLM

IPC: C12N15/10 ,  C12Q1/68 ,  C12N7/00

CPC classification number: C12Q1/6886 ,  C12N7/00 ,  C12N2795/18122 ,  C12N2795/18131 ,  C12Q2600/106 ,  C12Q2600/118

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

Abstract translation: 本发明的特征在于通过使用对照颗粒从生物样品中分离微泡或提取微囊泡核酸的方法和试剂盒。 本发明提供了控制颗粒，其是包含可以检测以评估微泡分离或核酸提取步骤的准确性，可靠性和效率的对照核酸的病毒或病毒样颗粒，例如噬菌体。 本文所述的方法还可以包括与用于诊断，预后或监测疾病或医疗状况的疾病或医学状况相关联的至少一种生物标志物的存在，不存在或水平的分析。

公开(公告)号：US20150038335A1

公开(公告)日：2015-02-05

申请号：US14357475

申请日：2012-11-12

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Leileata Russo

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1003 ,  C12N15/1017 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: The present invention is directed to methods of isolating particles, such as nucleic acid-containing particles or microvesicles, from a biological sample and extracting nucleic acids therefrom, wherein the biological sample is cerebrospinal fluid. The present invention further provides methods for aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker associated with a disease or medical condition thereof.

Abstract translation: 本发明涉及从生物样品中分离出颗粒如含核酸颗粒或微泡，并从其中提取核酸的方法，其中生物样品是脑脊液。 本发明还提供了通过检测与疾病或其医学状况相关的生物标志物来帮助诊断，预后，监测和评估受试者中的疾病或其他医学状况的方法。

公开(公告)号：US11899024B2

公开(公告)日：2024-02-13

申请号：US16629000

申请日：2018-07-12

Applicant: Exosome Diagnostics, Inc.

Inventor： Mia Sher ,  Erez Eitan ,  Christine Coticchia ,  Johan Karl Olov Skog ,  Robert Kitchen ,  Seth Yu

IPC: G01N33/68 ,  G01N33/537 ,  C12N15/10

CPC classification number: G01N33/6896 ,  C12N15/1013 ,  G01N33/537 ,  G01N2333/705 ,  G01N2800/28

Abstract: The invention relates generally to a process for isolating subpopulations of EVs to identify biomarkers useful identifying, determining the progression of, and/or prognosing a disease, including a neurological disease. More particularly, the present invention relates to detection technology of various exosomal biomarkers including proteins, protein modifications, sugars, RNA, DNA, lipids, and metabolites, and combinations thereof.

公开(公告)号：US20220355290A1

公开(公告)日：2022-11-10

申请号：US17621084

申请日：2020-06-24

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Frank NUSS ,  Daniel ENDERLE ,  Mikkel NOERHOLM

IPC: B01L3/00 ,  A61B10/00

Abstract: Described herein are various inventions and embodiments thereof, directed to systems, devices, and methods for separating a sample. Embodiments of sample collection devices disclosed herein may maintain separation of different volumes of sample during collecting, handling, and accessing of the sample. A sample collection device, in some embodiments, may comprise an enclosure defining a first chamber, a second chamber, and a partition therebetween configured to separate the first chamber and the second chamber. An assembly coupled to the partition may be configured to transition between an open configuration and a closed configuration based on fluid and gas flow through the assembly.

公开(公告)号：US11427864B2

公开(公告)日：2022-08-30

申请号：US16463820

申请日：2017-11-17

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Elena Castellanos-Rizaldos ,  Vasisht Tadigotla ,  Dominik Grimm ,  Xuan Zhang ,  Wei Yu

IPC: C12P19/34 ,  C12Q1/6851 ,  C12Q1/6886

Abstract: The present invention relates generally to methods and kits for detecting one or more biomarkers, such as an Epidermal Growth Factor Receptor (EGFR) mutation, e.g., T790M mutation, L858R mutation, one or more exon19 insertions and/or one or more exon19 deletions in the EGFR gene, in a biological sample to aid in diagnosis, prognosis, monitoring, or therapy selection for a disease such as, for example, cancer. The methods and kits are useful in aiding in diagnosis, prognosis, monitoring, or therapy selection for lung cancer, e.g., non-small cell lung cancer (NSCLC).

公开(公告)号：US20210238582A1

公开(公告)日：2021-08-05

申请号：US17074040

申请日：2020-10-19

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Daniel ENDERLE ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N15/10 ,  C12N15/11

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US20210230701A1

公开(公告)日：2021-07-29

申请号：US15734363

申请日：2019-06-06

Applicant: Exosome Diagnostics, Inc.

Inventor： Robert KITCHEN ,  Elena CASTELLANOS-RIZALDOS ,  Mia SHER ,  James HURLEY ,  Dalin CHAN ,  Georg STOLL ,  Wei YU ,  Johan SKOG

IPC: C12Q1/6886

Abstract: The present disclosure relates to methods for detecting and treating bladder cancer in a subject using the gene expression level measurements from exosomes derived from the subject.