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公开(公告)号：US20200317725A1
公开(公告)日：2020-10-08
申请号：US16911511
申请日：2020-06-25
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Annika Forss , Mats Ander , Tomas Bjorkman , Hans Blom , Jesper Hansson , Gustav Rodrigo
IPC: C07K1/16 , C07K16/00 , B01J20/286 , B01J20/285 , B01D15/18 , B01D15/34 , C07K1/22 , C07K14/31
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.
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公开(公告)号：US20200283475A1
公开(公告)日：2020-09-10
申请号：US16884475
申请日：2020-05-27
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Annika Kristina Forss , Gustav Jose Rodrigo , Tomas Bjorkman , Jesper Ulf Hansson , Mats Ander
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20200071353A1
公开(公告)日：2020-03-05
申请号：US16682855
申请日：2019-11-13
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , C07K16/00 , C07K14/31 , B01J20/28 , B01J20/24 , B01D15/38 , C07K17/10 , B01J20/286 , B01J20/32 , C07K16/06
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
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公开(公告)号：US20200023338A1
公开(公告)日：2020-01-23
申请号：US16337236
申请日：2017-09-27
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Ann Catharina Bergh , Tania Ahmad , Mattias Algotsson , Therese Graner
IPC: B01J20/289 , B01D15/38 , B01J20/28 , B01J20/32
Abstract: The present invention relates to a novel chromatography media, more closely a novel IMAC (Immobilized Metal Affinity Chromatography) media. The novel chromatography media comprises a pentaligand and provides high dynamic binding capacity as well as high purity of the sample proteins purified on the media of the invention.
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公开(公告)号：US20190241608A1
公开(公告)日：2019-08-08
申请号：US16386628
申请日：2019-04-17
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Jean-Luc Maloisel , Ola Lind , Bjorn Noren , Ronnie Palmgren
CPC classification number: C07K1/22 , C07K1/145 , C07K16/00 , C07K16/065 , C07K2317/55
Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.
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Patent Agency Ranking
26.发明授权公开(公告)号：US10343142B2
公开(公告)日：2019-07-09
申请号：US16189894
申请日：2018-11-13
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Martin Hall , Sture Larsson , Andreas Muranyi , Gustav Rodrigo , Jinyu Zou , Per-Mikael Aberg
IPC: B01J20/24 , B01D15/38 , B01J20/286 , C07K1/22 , B01J20/32 , C07K14/31 , C07K16/00 , C07K17/00 , B01J20/28 , B01J20/285 , B01J20/289 , C07K17/10
Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.
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公开(公告)号：US10265697B2
公开(公告)日：2019-04-23
申请号：US15292093
申请日：2016-10-12
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Karol Lacki
Abstract: A micro titre plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.
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公开(公告)号：US10208092B2
公开(公告)日：2019-02-19
申请号：US15526895
申请日：2015-12-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC: C07K14/195 , C07K1/22 , C07K16/00
Abstract: The invention discloses kappa light chain-binding polypeptide comprising a mutated binding domain of Peptostreptococcus protein L, wherein at least one asparagine residue of a parental domain defined by, or having at least 95% or 98% sequence homology with, SEQ ID NO: 2-6 or 2 has been mutated to another amino acid residue which is not asparagine, proline or cysteine.
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公开(公告)号：US20190038996A1
公开(公告)日：2019-02-07
申请号：US16154639
申请日：2018-10-08
Applicant: GE Healthcare BioProcess R&D AB
Inventor： KLAUS GEBAUER
Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.
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公开(公告)号：US10048235B2
公开(公告)日：2018-08-14
申请号：US15379447
申请日：2016-12-14
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Klaus Gebauer
Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.
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