公开(公告)号：US20110126318A1

公开(公告)日：2011-05-26

申请号：US12917024

申请日：2010-11-01

Applicant: Stephen M. Allen ,  Howard Glenn Damude ,  John D. Everard ,  Knut Meyer ,  Byung-Chun Yoo

Inventor： Stephen M. Allen ,  Howard Glenn Damude ,  John D. Everard ,  Knut Meyer ,  Byung-Chun Yoo

IPC: A01H5/10 ,  A01H5/00 ,  C12N15/82 ,  C07H21/04 ,  C07K14/415 ,  C12Q1/68 ,  C12N9/10

CPC classification number: C12N15/8247 ,  C07K14/415 ,  C12N9/1029 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Y203/0102

Abstract: This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.

Abstract translation: 本发明涉及编码SUT2或SUT4蔗糖转运蛋白基因的多核苷酸序列。 描述了增加种子油含量和评估植物种子中增加的油含量的方法。 本文公开的组合物和方法使用编码蔗糖转运蛋白的多种序列和影响脂肪酸积累的多种序列，包括例如DGAT，Lec1和ODP1转录因子。 在具体实施方案中，与包含不过表达SUT2或SUT4转运蛋白的重组DNA构建体的高油植物相比，SUT2和/或SUT4蔗糖转运蛋白与DGAT基因的过表达进一步增加植物种子油产量。

公开(公告)号：US07932077B2

公开(公告)日：2011-04-26

申请号：US11265761

申请日：2005-11-02

Applicant: Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC: C12N1/16 ,  C12N9/02 ,  C12N9/08 ,  C12P7/64 ,  C07H21/04

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

Abstract translation: 描述了能够在总油馏分中产生大于25％二十碳五烯酸（EPA，ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包括表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使EPA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内生产EPA的方法。

公开(公告)号：US20110016581A1

公开(公告)日：2011-01-20

申请号：US12445633

申请日：2007-10-22

Applicant: Howard Glenn Damude ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C12N15/82 ,  C07H21/00 ,  C12N9/02 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10 ,  C07F9/10 ,  C12P7/64 ,  C07C57/02 ,  A23L1/20 ,  A23K1/00

CPC classification number: C12N15/8247 ,  A23K20/158 ,  A23L33/12 ,  C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-8 desaturases in plants and oleaginous yeast.

公开(公告)号：US20090313720A1

公开(公告)日：2009-12-17

申请号：US12298563

申请日：2007-04-26

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: C12N15/87 ,  C12Q1/68 ,  C12N5/10 ,  C07H21/00 ,  C07C57/03 ,  A01H5/00

CPC classification number: C12N15/8247 ,  C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.

Abstract translation: 公开了分离的核酸片段和重组构建体，其包含编码Δ-8去饱和酶的片段以及使用该植物中的δ-8去饱和酶和油性酵母制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090293152A1

公开(公告)日：2009-11-26

申请号：US12470569

申请日：2009-05-22

Applicant: KEITH ROESLER ,  HOWARD GLENN DAMUDE ,  CHANGJIANG LI ,  KNUT MEYER ,  BO SHEN ,  ERICKA BERMUDEZ ,  MITCHELL C. TARCZYNSKI

Inventor： KEITH ROESLER ,  HOWARD GLENN DAMUDE ,  CHANGJIANG LI ,  KNUT MEYER ,  BO SHEN ,  ERICKA BERMUDEZ ,  MITCHELL C. TARCZYNSKI

IPC: A01H5/00 ,  C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12Q1/04 ,  C12N1/19

CPC classification number: C12N15/8247 ,  A23K10/30 ,  A23L11/00 ,  A23V2002/00 ,  C12N9/1029 ,  C12Y203/0102

Abstract: Transgenic oilseeds having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of null segregant oilseeds are described. Novel DGAT genes are used to achieve the increase in seed storage lipids.

Abstract translation: 描述了与无分离油籽油的总脂肪酸含量相比，具有总脂肪酸含量增加至少10％和改变的脂肪酸分布的转基因油籽。 新型DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US07550286B2

公开(公告)日：2009-06-23

申请号：US11264737

申请日：2005-11-01

Applicant: Howard Glenn Damude ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  James John Ragghianti ,  John E. Seip ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  James John Ragghianti ,  John E. Seip ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC: C12N1/16 ,  C12P7/64 ,  C12N9/02 ,  C07H21/04

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

Abstract translation: 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。

公开(公告)号：US20080286436A1

公开(公告)日：2008-11-20

申请号：US11749859

申请日：2007-05-17

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: A23K1/14 ,  C12N15/52 ,  C12N5/10 ,  C12N1/19 ,  C12P7/64 ,  A01H5/00 ,  A23L1/212

CPC classification number: C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-5 desaturase in plants and oleaginous yeast are disclosed.

Abstract translation: 公开了分离的核酸片段和包含编码δ-5去饱和酶的片段的重组构建物，以及在植物和油质酵母中使用该δ-5去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20080254521A1

公开(公告)日：2008-10-16

申请号：US12099811

申请日：2008-04-09

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: C12P7/64 ,  C12N1/21 ,  C12N1/15 ,  C12N15/52 ,  C12N1/13 ,  C12N1/19

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N15/8247

Abstract: The present invention relates to Δ8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ω-6) to dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) and/or eicosatrienoic acid (ETrA; 20:3 ω-3) to eicosatetraenoic acid (ETA; (20:3 ω-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding Δ8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ8 desaturases in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将二十碳二烯酸（EDA; 20：2ω-6）转化成二高-γ-亚麻酸（DGLA; 20：3ω-6）和/或二十碳三烯酸（ 分离的核酸片段和包含编码Delta8去饱和酶的片段的重组DNA构建体以及制备长链多不饱和脂肪酸的方法的分离的核酸片段和重组DNA构建体（ETA;（20：3ω-3） （PUFA）在含油酵母中使用这些Delta8去饱和酶。

公开(公告)号：US20080254192A1

公开(公告)日：2008-10-16

申请号：US12099799

申请日：2008-04-09

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: A23D7/00 ,  C12N15/11 ,  C12N9/00 ,  C12N15/00 ,  C12N5/04 ,  C12N15/82 ,  A23L1/20 ,  C11B1/00 ,  C12P7/64 ,  A01H5/00 ,  A23K1/00

CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N15/8247

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-8 desaturases in plants.

Abstract translation: 分离的核酸片段和包含编码δ-8去饱和酶的片段以及制备长链多不饱和脂肪酸（PUFA）的方法并在植物中使用这些δ-8去饱和酶的重组构建体。

公开(公告)号：US20080118623A1

公开(公告)日：2008-05-22

申请号：US11737772

申请日：2007-04-20

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: A23D7/00 ,  C12N15/11 ,  C12N9/00 ,  C12N15/00 ,  C12P7/64 ,  C12N5/04 ,  A23K1/00 ,  A23L1/36 ,  C12N1/00 ,  C12N15/87 ,  C12N15/82 ,  A01H5/00 ,  C11B1/00

CPC classification number: C12N15/8247 ,  C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.

Abstract translation: 公开了分离的核酸片段和重组构建体，其包含编码Δ-8去饱和酶的片段以及使用该植物中的δ-8去饱和酶和油性酵母制备长链多不饱和脂肪酸（PUFA）的方法。