公开(公告)号：US08999681B2

公开(公告)日：2015-04-07

申请号：US13991929

申请日：2011-12-08

申请人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

发明人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

IPC分类号： C12P13/00 ,  C12N9/04 ,  C12N9/88

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/88 ,  C12Y101/01003 ,  C12Y401/01018

摘要： A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.

摘要翻译： 与通过常规发酵方法的生产方法相比，生产尸胺的方法更有效并且产量高。 该方法包括培养耐pH为5.5以下的棒状细菌/细菌。 优选地，棒状细菌/细菌具有/具有赖氨酸脱羧酶活性，优选棒状细菌/细菌具有高丝氨酸营养缺陷型和/或对S-（2-氨基乙基）-L-半胱氨酸的抗性。

公开(公告)号：US08871477B2

公开(公告)日：2014-10-28

申请号：US13580742

申请日：2011-02-22

申请人： Takashi Mimitsuka ,  Kazumi Suda ,  Hideki Sawai

发明人： Takashi Mimitsuka ,  Kazumi Suda ,  Hideki Sawai

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C07K2319/02 ,  C12N9/88

摘要： By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced.

摘要翻译： 通过培养细胞外分泌赖氨酸脱羧酶的微生物来制造尸胺的方法，抑制赖氨酸的副产生，与常规生产方法相比，尸胺相对于葡萄糖消耗的产率提高，此外，纯化步骤中的负荷 可以降低作为聚酰胺原料的尸胺的净化。

公开(公告)号：US08558037B2

公开(公告)日：2013-10-15

申请号：US13076052

申请日：2011-03-30

申请人： Izumi Morita ,  Masateru Ito ,  Hideki Sawai ,  Shinichi Minegishi ,  Katsushige Yamada ,  Kenji Kawamura

发明人： Izumi Morita ,  Masateru Ito ,  Hideki Sawai ,  Shinichi Minegishi ,  Katsushige Yamada ,  Kenji Kawamura

IPC分类号： C07C29/74

CPC分类号： C07C29/76 ,  C07C31/12

摘要： A method of producing butanol, which has a step of removing impurities contained in a butanol-containing solution, is provided. In the method, a butanol-containing solution is filtered through a nanofiltration membrane. Then the butanol-containing solution is collected from the permeate flow of the nanofiltration membrane.

摘要翻译： 提供一种生产丁醇的方法，其具有除去含丁醇溶液中所含的杂质的步骤。 在该方法中，将含丁醇的溶液通过纳滤膜过滤。 然后从纳滤膜的渗透流中收集含丁醇的溶液。

公开(公告)号：US20110177551A1

公开(公告)日：2011-07-21

申请号：US13121727

申请日：2009-09-16

申请人： Takashi Mimitsuka ,  Kentaro Ishii ,  Ken Morita ,  Masashi Higasa ,  Kenji Sawai ,  Hideki Sawai ,  Katsushige Yamada ,  Shinichi Minegishi

发明人： Takashi Mimitsuka ,  Kentaro Ishii ,  Ken Morita ,  Masashi Higasa ,  Kenji Sawai ,  Hideki Sawai ,  Katsushige Yamada ,  Shinichi Minegishi

IPC分类号： C12P1/00 ,  C12M1/12

CPC分类号： C12P7/56 ,  C12M29/18 ,  C12M47/10 ,  C12N9/00 ,  C12P1/00 ,  C12P7/02 ,  C12P7/40 ,  C12P13/001 ,  C12P13/04 ,  C12P13/08 ,  C12P19/34 ,  C12P21/02

摘要： To control flow velocity of a culture liquid inside a membrane separation tank without giving influences to culture conditions in the fermentation tank, and also suppress precipitation of microorganisms or culture cells so that the production efficiency of the chemical product can be improved, in a method for producing a chemical product including the steps of: cultivating microorganisms or culture cells in a fermentation tank; transferring a culture liquid from the fermentation tank to a membrane separation tank so as to filter the culture liquid through a separation membrane; and collecting a fermentation product from a filtration liquid as the chemical product while refluxing an unfiltered culture liquid that has not been filtered so as to be joined to the culture liquid on an upstream side of the membrane separation tank, one portion of the culture liquid to be transferred from the fermentation tank is allowed to bypass the membrane separation tank depending on a pressure at the culture liquid flow-in side of the membrane separation tank.

摘要翻译： 为了控制膜分离槽内的培养液的流速，不影响发酵罐中的培养条件，并且还抑制微生物或培养细胞的沉淀，从而可以提高化学品的生产效率 生产化学产品，包括以下步骤：在发酵罐中培养微生物或培养细胞; 将培养液从发酵罐转移到膜分离罐中，以通过分离膜过滤培养液; 并将来自过滤液的发酵产物作为化学产品收集，同时回流未过滤的未过滤的培养液，以便在膜分离罐的上游侧与培养液接合，将一部分培养液转移至 根据膜分离罐的培养液流入侧的压力，从发酵罐转移允许绕过膜分离罐。

公开(公告)号：US20090269812A1

公开(公告)日：2009-10-29

申请号：US12280197

申请日：2007-02-16

申请人： Hideki Sawai ,  Katsushige Yamada ,  Takashi Mimitsuka ,  Kenji Sawai ,  Tetsu Yonehara ,  Yohito Ito ,  Masahiro Henmi

发明人： Hideki Sawai ,  Katsushige Yamada ,  Takashi Mimitsuka ,  Kenji Sawai ,  Tetsu Yonehara ,  Yohito Ito ,  Masahiro Henmi

IPC分类号： C12P19/40 ,  C12P1/04 ,  C12P7/56 ,  C12P7/46 ,  C12P7/52 ,  C12P7/02 ,  C12P13/00 ,  C12P7/06 ,  C12P7/18 ,  C12P19/30 ,  C12P13/04 ,  C12P13/08 ,  C12M1/36

CPC分类号： C12P7/06 ,  C12M29/04 ,  C12M29/18 ,  C12M41/40 ,  C12M47/10 ,  C12P7/18 ,  C12P7/46 ,  C12P7/50 ,  C12P7/56 ,  C12P13/001 ,  C12P13/005 ,  C12P13/04 ,  C12P13/08 ,  C12P19/30 ,  Y02E50/17

摘要： The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 μm or more to less than 1 μm is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost.

摘要翻译： 本发明提供了一种通过连续发酵生产化学产品的方法，包括用分离膜过滤微生物培养物或培养细胞，从滤液中回收产物，同时将非过滤液体保留在培养基中或将其回流至培养物中 将发酵材料添加到培养物中，其中使用平均孔径为0.01μm以上且小于1μm的多孔膜作为分离膜，并且以跨膜压差为0.1〜 20 kPa。 根据该方法，化学产品的发酵生产率可以在高稳定性和低成本的情况下大大提高。

公开(公告)号：US10683254B2

公开(公告)日：2020-06-16

申请号：US13142062

申请日：2009-12-25

申请人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

发明人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

IPC分类号： C07C59/08 ,  B01D71/56 ,  C08G63/06 ,  C12P7/56 ,  C07C51/42 ,  B01D61/02

摘要： Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.

公开(公告)号：US09080190B2

公开(公告)日：2015-07-14

申请号：US13992081

申请日：2011-12-08

申请人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

发明人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C12Y401/01018

摘要： A method produces cadaverine more efficiently and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having an ability to produce cadaverine and having a resistance to 2,2′-thiobis(ethylamine). Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.

摘要翻译： 一种方法通过常规发酵方法比生产方法更有效地产生尸胺，并且产量高。 该方法包括培养具有产生尸胺的能力并具有对2,2'-硫代双（乙胺）的抗性的棒状细菌/细菌。 优选地，棒状细菌/细菌具有/具有赖氨酸脱羧酶活性，优选棒状细菌/细菌具有高丝氨酸营养缺陷型和/或对S-（2-氨基乙基）-L-半胱氨酸的抗性。

公开(公告)号：US08859260B2

公开(公告)日：2014-10-14

申请号：US12746642

申请日：2008-12-05

申请人： Kenji Sawai ,  Hideki Sawai

发明人： Kenji Sawai ,  Hideki Sawai

IPC分类号： C12N1/19 ,  C12N9/04 ,  C12P7/56 ,  C12N15/81

CPC分类号： C12P7/56 ,  C12N9/0006 ,  C12N15/81

摘要： A gene expressing cassette codes lactate dehydrogenase that is needed for prevention of deterioration in lactic acid yield and lactic acid production rate in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability, which achieves high optical purity, high lactic acid yield and high lactic acid production rate simultaneously, a yeast strain having the cassette and a method of producing lactic acid by culturing the yeast strain. The lactate dehydrogenase-expressing cassette is a lactate dehydrogenase-expressing cassette, comprising a gene coding lactate dehydrogenase connected to a site downstream of a promoter, the promoter being a promoter of a gene showing a gene expression amount larger by 5 times or more than the average relative expression amount of all genes after 50 hours from start of culture in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability.

摘要翻译： 表达盒的基因编码乳酸脱氢酶，其在连续培养中同时过滤具有乳酸生产能力的酵母菌株（其获得高光学纯度）的高乳酸所需的乳酸产率和乳酸生产率的降低所需的乳酸脱氢酶 产量和高乳酸生产率，具有盒的酵母菌株和通过培养酵母菌株产生乳酸的方法。 表达乳酸脱氢酶的盒是表达乳酸脱氢酶的盒，其包含编码与启动子下游位点连接的乳酸脱氢酶的基因，该启动子是基因表达量大于5倍以上的基因的启动子 同时过滤具有乳酸生产能力的酵母菌株的连续培养开始培养50小时后的所有基因的平均相对表达量。

公开(公告)号：US20130071888A1

公开(公告)日：2013-03-21

申请号：US13640793

申请日：2011-04-11

申请人： Kenji Sawai ,  Shiomi Watanabe ,  Takashi Mimitsuka ,  Hideki Sawai

发明人： Kenji Sawai ,  Shiomi Watanabe ,  Takashi Mimitsuka ,  Hideki Sawai

IPC分类号： C12N15/52

CPC分类号： C12N15/52 ,  C12N9/88 ,  C12P13/001 ,  C12Y401/01018

摘要： A method of producing 1,5-pentanediamine includes culturing coryneform bacterium having a gene encoding lysine decarboxylase in its chromosome, which coryneform bacterium maintains lysine decarboxylase activity of not less than 50 mU/mg protein during culturing and the gene encoding lysine decarboxylase is linked downstream of a promoter that functions during the logarithmic growth phase.

摘要翻译： 一种生产1,5-戊二胺的方法包括在其染色体中培养具有编码赖氨酸脱羧酶基因的棒状细菌，棒状细菌在培养期间保持不低于50mU / mg蛋白质的赖氨酸脱羧酶活性，编码赖氨酸脱羧酶的基因连接在下游 在对数生长期间起作用的启动子。

公开(公告)号：US08334411B2

公开(公告)日：2012-12-18

申请号：US12920891

申请日：2009-03-11

申请人： Masateru Ito ,  Izumi Nakagawa ,  Koya Kato ,  Takashi Mimitsuka ,  Kenji Sawai ,  Shin-ichi Minegishi ,  Hideki Sawai ,  Katsushige Yamada

发明人： Masateru Ito ,  Izumi Nakagawa ,  Koya Kato ,  Takashi Mimitsuka ,  Kenji Sawai ,  Shin-ichi Minegishi ,  Hideki Sawai ,  Katsushige Yamada

IPC分类号： C07C209/84 ,  C07C209/86 ,  C07C231/02

CPC分类号： C07C209/84 ,  B01D61/025 ,  B01D61/027 ,  B01D61/58 ,  B01D71/60 ,  B01D2317/022 ,  C08G69/26 ,  C08G69/28 ,  C07C211/09 ,  C07C211/12

摘要： A method for producing a diamine includes purifying a diamine from an aqueous solution containing a diamine salt by adding an alkaline substance to the aqueous solution and then filtering the resulting solution by allowing the solution to pass through a nanofiltration membrane to remove the salt, thereby obtaining an aqueous diamine solution.

摘要翻译： 二胺的制造方法包括：通过向水溶液中添加碱性物质，从含有二胺盐的水溶液中纯化二胺，然后通过使溶液通过纳滤膜除去盐而过滤所得溶液，从而得到 二胺水溶液。