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    Mature apoai protein production under serum free culturing conditions
    24.
    发明授权
    Mature apoai protein production under serum free culturing conditions 失效
    在无血清培养条件下成熟的脱水蛋白生产

    公开(公告)号：US4943527A

    公开(公告)日：1990-07-24

    申请号：US834300

    申请日：1986-02-27

    Applicant: Andrew A. Protter ,  Jean-Louis Vigne ,  Joanne B. Mallory ,  Karen D. Talmadge ,  John P. Kane

    Inventor： Andrew A. Protter ,  Jean-Louis Vigne ,  Joanne B. Mallory ,  Karen D. Talmadge ,  John P. Kane

    IPC: A61K31/00 ,  C07K14/775 ,  C12N15/62 ,  C12N15/85 ,  C12P21/02 ,  C12P21/04

    CPC classification number: C07K14/775 ,  A61K31/00 ,  C12N15/62 ,  C12N15/85 ,  C12P21/02 ,  C07K2319/02 ,  C07K2319/75

    Abstract: A method of producing a purified lipid-binding peptide which can bind to phospholipids at one or more amphipatic alpha-helical peptide regions. The method includes providing a gene coding for the peptide, and introducing the gene in expressible, heterologous form in a suitable expression system capable of synthesizing a mixture of peptides which includes the lipid-binding peptide. Addition of either endogenous or exogenous lipids to the peptide mixture forms a low-density lipopeptide complex composed of lipid and the lipid-binding peptide, and this complex can be separated easily from nonlipid-binding peptides in the peptide on the basis of its size and/or density. The method is intended particularly for scaled-up production of purified human apolipoproteins and their alpha-helical lipid-binding regions. Also disclosed are related methods for producing recombinant apolipoproteins, therapeutic lipopeptide compositions, and a stabilized lipid emulsion for nutritional therapy. Further disclosed are methods for expressing apolipoproteins or lipid-binding segments thereof in bacterial, yeast and mammalian cell expression systems, and methods for purifying lipid binding proteins, including fused recombinant proteins.

    Abstract translation: 一种产生纯化的脂质结合肽的方法，其可以在一个或多个两亲性α-螺旋肽区域结合磷脂。 该方法包括提供编码该肽的基因，并将该基因以可表达的异源形式引入到能够合成包含该脂质结合肽的肽混合物的合适的表达系统中。 将内源或外源脂质添加到肽混合物中形成由脂质和脂质结合肽组成的低密度脂肽复合物，并且该复合物可以容易地从肽中的非脂蛋白结合肽分离，基于其大小和 /或密度。 该方法特别用于放大生产纯化的人载脂蛋白及其α-螺旋脂质结合区域。 还公开了用于生产重组载脂蛋白，治疗性脂肽组合物和用于营养治疗的稳定的脂质乳剂的相关方法。 还公开了用于在细菌，酵母和哺乳动物细胞表达系统中表达载脂蛋白或其脂质结合片段的方法，以及用于纯化包含融合重组蛋白质的脂质结合蛋白的方法。

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