公开(公告)号：US09797010B2

公开(公告)日：2017-10-24

申请号：US12809120

申请日：2008-12-19

申请人： David A. Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

发明人： David A. Weitz ,  Jeremy Agresti ,  Michael P. Weiner ,  Adam R. Abate ,  Tony Hung

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q2537/143 ,  C12Q2563/149

摘要： The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.

公开(公告)号：US20100305004A1

公开(公告)日：2010-12-02

申请号：US12679586

申请日：2008-09-26

申请人： Michael P. Weiner ,  Michael I. Sherman

发明人： Michael P. Weiner ,  Michael I. Sherman

IPC分类号： C40B50/06 ,  C07K14/00 ,  C12N9/00 ,  G01N33/50

CPC分类号： C12P21/02 ,  C07K14/003 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/00 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/682 ,  G01N2458/10 ,  C12Q2563/179 ,  C12Q2521/501

摘要： We use the Tus-Ter interaction to enable the utilization of nucleic acid analytical methodologies for proteins. We also use the Tus-Ter interaction to make polymers and oligomers that have a nucleic acid backbone with protein functionalities. These methods are useful for molecular modeling, for efficiently running enzymatic pathway reactions, and for analyzing presence and/or amount of particular proteins.

摘要翻译： 我们使用Tus-Ter相互作用来实现对蛋白质的核酸分析方法的利用。 我们还使用Tus-Ter相互作用来制备具有蛋白质功能的核酸骨架的聚合物和低聚物。 这些方法可用于分子建模，有效运行酶途径反应，以及分析特定蛋白质的存在和/或数量。

公开(公告)号：US20090011959A1

公开(公告)日：2009-01-08

申请号：US12154901

申请日：2008-05-27

申请人： Gina L. Costa ,  John H. Leamon ,  Jonathan M. Rothberg ,  Michael P. Weiner

发明人： Gina L. Costa ,  John H. Leamon ,  Jonathan M. Rothberg ,  Michael P. Weiner

IPC分类号： C40B50/14

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

摘要： This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.

摘要翻译： 本发明涉及产生用于扩增和测序反应的单链DNA文库的方法。 在各方面，所公开的方法包括：片段化DNA; 抛光片段的末端; 将碎片连接到通用适配器; 进行缝合位移和切口碎片的延伸; 纯化双链结合产物; 将双链结合产物捕获到固体支持物上; 并分离单链DNA文库片段，并将这些片段结合到另一种固体支持物上。

公开(公告)号：US07323305B2

公开(公告)日：2008-01-29

申请号：US10767779

申请日：2004-09-22

申请人： John H. Leamon ,  Kenton L. Lohman ,  Jonathan M. Rothberg ,  Michael P. Weiner

发明人： John H. Leamon ,  Kenton L. Lohman ,  Jonathan M. Rothberg ,  Michael P. Weiner

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34 ,  C12M1/40 ,  C07H21/04 ,  C12Q1/66 ,  C12N11/18

CPC分类号： C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.

摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。

公开(公告)号：US5523221A

公开(公告)日：1996-06-04

申请号：US78662

申请日：1993-06-16

申请人： Michael P. Weiner

发明人： Michael P. Weiner

IPC分类号： C12N15/66 ,  C12N15/11

CPC分类号： C12N15/66

摘要： A method for directionally cloning an insert DNA fragment into a target sequence using differential phosphorylation is disclosed, Monophosphorylated PCR fragments are directionally cloned into a monophosphorylated plasmid, Methods for directionally cloning non-PCR fragments into target DNA sequences are also discussed.

摘要翻译： 公开了使用差异磷酸化将插入DNA片段定向克隆到靶序列中的方法，单磷酸化PCR片段被定向克隆到单磷酸化质粒中。还讨论了将非PCR片段定向克隆到靶DNA序列中的方法。