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    SYSTEM AND METHODS
    21.
    发明申请
    SYSTEM AND METHODS 有权

    公开(公告)号：US20210308682A1

    公开(公告)日：2021-10-07

    申请号：US17349068

    申请日：2021-06-16

    Applicant: SPHERE FLUIDICS LIMITED

    Inventor： Frank F. CRAIG ,  Marian Rehak ,  David Holmes ,  Clive A. Smith ,  Xin Liu ,  Giuseppe Benazzi ,  Xin Li ,  Vinayaka Pawate ,  Robert Frank Marchington ,  Richard Francis Day ,  Michael Stewart Hazell

    IPC: B01L3/00 ,  G01N35/08 ,  G01N15/14 ,  C12M1/00

    Abstract: We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid: preparing a droplet from said fluid: determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.

    DROPLET PROCESSING METHODS AND SYSTEMS
    22.
    发明申请
    DROPLET PROCESSING METHODS AND SYSTEMS 有权

    公开(公告)号：US20210293691A1

    公开(公告)日：2021-09-23

    申请号：US17266447

    申请日：2019-08-06

    Applicant: Sphere Fluidics Limited

    Inventor： Nicholas Dayrell-Armes ,  David Holmes ,  Frank F Craig ,  Marian Rehak ,  Dimitris Josephides ,  Robert Salter ,  William Whitley ,  Sinan Gokkaya ,  Raphael Ruis

    IPC: G01N15/14 ,  B01L3/00 ,  G06K9/00 ,  G06K9/62 ,  G06T7/70

    Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

    DROPLET SORTING
    24.
    发明申请
    DROPLET SORTING 审中-公开

    公开(公告)号：US20170227440A1

    公开(公告)日：2017-08-10

    申请号：US15502630

    申请日：2015-08-07

    Applicant: Sphere Fluidics Limited

    Inventor： Clive Adrian Smith ,  Xin Li ,  Frank F. Craig

    IPC: G01N15/10 ,  B01L3/00

    Abstract: We describe a method comprising: providing a droplet comprising a plurality of constituents, splitting said droplet into a first droplet and a second droplet, wherein said first droplet comprises a first fraction of said plurality of constituents and said second droplet comprises a second fraction of said plurality of constituents, analysing said constituents of said first fraction of said plurality of constituents in said first droplet, and sorting said second droplet dependent on an outcome of said analysis.

    MICROFLUIDIC DEVICES AND SYSTEMS
    26.
    发明申请
    MICROFLUIDIC DEVICES AND SYSTEMS 审中-公开
    微流体装置和系统

    公开(公告)号：US20160252446A1

    公开(公告)日：2016-09-01

    申请号：US14907867

    申请日：2014-07-30

    Applicant: Sphere Fluidics Limited

    Inventor： Clive A. SMITH ,  Xin LI ,  Karine Enesa ,  Xin LIU ,  Frank F. CRAIG ,  Graeme WHYTE

    IPC: G01N15/14

    CPC classification number: G01N15/1434 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  C12Q1/04 ,  C12Q1/06 ,  G01N15/1425 ,  G01N21/49 ,  G01N2015/0065 ,  G01N2015/149 ,  Y10T436/2575

    Abstract: We describe a method of label-free cell and/or particle sorting in a microfluidic device, the method comprising; providing a stream of aqueous droplets in oil in a channel of said microfluidic device, wherein at least some of said droplets include cells and/or particles illuminating said stream from a first direction; detecting, for example, scattered light from cells and/or particles within said aqueous droplets in a second direction; determining, for example, a number of said cells and/or particles in each droplet from the scattered light; and sorting said aqueous droplets into one of a plurality of differentiated streams responsive to said determined number. The method further comprises modifying a refractive index of said oil to more closely match a refractive index of said aqueous droplets to reduce light scattered from boundaries of said droplets. Optionally a volume of the droplets is also controlled. In embodiments the oil is fluorous oil. We describe examples of preferred refractive index modifying compounds.

    Abstract translation: 我们描述了在微流体装置中无标记细胞和/或颗粒分选的方法，该方法包括： 在所述微流体装置的通道中提供油中的水滴液滴，其中至少一些所述液滴包括从第一方向照射所述流的细胞和/或颗粒; 从第二方向检测来自所述水滴中的细胞和/或颗粒的散射光; 确定例如来自散射光的每个液滴中的所述细胞和/或颗粒的数量; 以及响应于所述确定的数量将所述水滴分选成多个分化流中的一个。 该方法还包括改变所述油的折射率以更紧密地匹配所述水滴的折射率，以减少从所述液滴的边界散射的光。 任选地，还控制一定体积的液滴。 在实施方案中，油是氟油。 我们描述优选折射率修饰化合物的实例。

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