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公开(公告)号：US12084716B2
公开(公告)日：2024-09-10
申请号：US17473760
申请日：2021-09-13
申请人： 10X Genomics, Inc.
发明人： Phillip Belgrader , Zachary Bent , Rajiv Bharadwaj , Vijay Kumar Sreenivasa Gopalan , Josephine Harada , Christopher Hindson , Mohammad Rahimi Lenji , Michael Ybarra Lucero , Geoffrey McDermott , Elliott Meer , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Serge Saxonov , John R. Stuelpnagel , Jessica Michele Terry , Tobias Daniel Wheeler , Indira Wu , Solongo Batjargal Ziraldo , Stephane Claude Boutet , Sarah Taylor , Niranjan Srinivas
IPC分类号： C12Q1/68 , C12Q1/6806 , C12Q1/683 , C12Q1/6874
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/683 , C12Q2525/191 , C12Q2537/143 , C12Q2563/179
摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
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公开(公告)号：US11636921B2
公开(公告)日：2023-04-25
申请号：US16698612
申请日：2019-11-27
申请人： 10X Genomics, Inc.
发明人： Michael Schnall-Levin , Rajiv Bharadwaj , Zahra Kamila Belhocine , Andrew D. Price , Yifeng Yin , Vijay Kumar Sreenivasa Gopalan , Zeljko Jovan Dzakula
摘要： Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.
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公开(公告)号：US11193122B2
公开(公告)日：2021-12-07
申请号：US17208369
申请日：2021-03-22
申请人： 10X Genomics, Inc.
发明人： Zahra Kamila Belhocine , Rajiv Bharadwaj , Christopher Hindson , Michael Schnall-Levin , Bill Kengli Lin , Anthony Makarewicz , Pranav Patel , Andrew D. Price , Mohammad Rahimi Lenji , Tobias Daniel Wheeler , Yifeng Yin
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869
摘要： Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.
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公开(公告)号：US20210222158A1
公开(公告)日：2021-07-22
申请号：US17208369
申请日：2021-03-22
申请人： 10X Genomics, Inc.
发明人： Zahra Kamila Belhocine , Rajiv Bharadwaj , Christopher Hindson , Michael Schnall-Levin , Bill Kengli Lin , Anthony Makarewicz , Pranav Patel , Katherine Pfeiffer , Andrew D. Price , Mohammad Rahimi Lenji , Tobias Daniel Wheeler , Yifeng Yin
IPC分类号： C12N15/10 , C12Q1/6806 , C12Q1/6869
摘要： Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.
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公开(公告)号：US20210040535A1
公开(公告)日：2021-02-11
申请号：US16915493
申请日：2020-06-29
申请人： 10X GENOMICS, INC.
发明人： Adam Lowe , Christopher Hindson , Andrew D. Price
IPC分类号： C12Q1/6806 , C12N15/10 , B01J19/00 , B01J13/04
摘要： The present disclosure provides particles (e.g., beads) and methods, kits, and systems involving the same for sample processing or analysis. Such particles may include one or more analytes, one or more reagents, and two or more gel components and/or walled components. The particles described herein may be formed, for example, by polymerization of a polymerizable material in proximity to a gel or walled component.
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公开(公告)号：US20200239874A1
公开(公告)日：2020-07-30
申请号：US16554564
申请日：2019-08-28
申请人： 10X GENOMICS, INC.
发明人： Tarjei Sigurd Mikkelsen , Eswar Prasad Ramachandran Iyer , Andrew Kohlway , Luigi Jhon Alvarado Martinez , Katherine Pfeiffer , Andrew D. Price
IPC分类号： C12N15/10 , C12Q1/682 , C12Q1/6855
摘要： The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.
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公开(公告)号：US10590244B2
公开(公告)日：2020-03-17
申请号：US16178430
申请日：2018-11-01
申请人： 10X Genomics, Inc.
摘要： The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.
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公开(公告)号：US20200002763A1
公开(公告)日：2020-01-02
申请号：US16439675
申请日：2019-06-12
申请人： 10X Genomics, Inc.
发明人： Phillip Belgrader , Zachary Bent , Rajiv Bharadwaj , Vijay Kumar Sreenivasa Gopalan , Josephine Harada , Christopher Hindson , Mohammad Rahimi Lenji , Michael Ybarra Lucero , Geoffrey McDermott , Elliott Meer , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Serge Saxonov , John R. Stuelpnagel , Jessica Michele Terry , Tobias Daniel Wheeler , Indira Wu , Solongo Batjargal Ziraldo , Stephane Claude Boutet , Sarah Taylor , Niranjan Srinivas
IPC分类号： C12Q1/6874 , C12Q1/683 , C12Q1/6806
摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
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29.发明申请公开(公告)号：US20190383804A1
公开(公告)日：2019-12-19
申请号：US16375119
申请日：2019-04-04
申请人： 10X Genomics, Inc.
发明人： Kamila Belhocine , Jason Bell , Zachary Bent , Rajiv Bharadwaj , Christopher Hindson , Mohammad Rahimi Lenji , Bill Kengli Lin , Anthony Makarewicz , Geoffrey McDermott , Elliott Meer , Francesca Meschi , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Michael Schnall-Levin , Sarah Taylor , Jessica Michele Terry , Tobias Daniel Wheeler , Yifeng Yin , Xinying Zheng , Solongo Batjargal Ziraldo , Eswar Prasad Ramachandran Iyer , Luigi Jhon Alvarado Martinez
IPC分类号： G01N33/53 , G01N33/543 , C12N15/11
摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.
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公开(公告)号：US20190367969A1
公开(公告)日：2019-12-05
申请号：US16375093
申请日：2019-04-04
申请人： 10X Genomics, Inc.
发明人： Kamila Belhocine , Jason Bell , Zachary Bent , Rajiv Bharadwaj , Christopher Hindson , Mohammad Rahimi Lenji , Bill Kengli Lin , Anthony Makarewicz , Geoffrey McDermott , Elliott Meer , Francesca Meschi , Tarjei Sigurd Mikkelsen , Christopher Joachim O'Keeffe , Katherine Pfeiffer , Andrew D. Price , Paul Ryvkin , Michael Schnall-Levin , Sarah Taylor , Jessica Michele Terry , Tobias Daniel Wheeler , Yifeng Yin , Xinying Zheng , Solongo Batjargal Ziraldo , Eswar Prasad Ramachandran Iyer , Luigi Jhon Alvarado Martinez
IPC分类号： C12Q1/6818 , C12Q1/6827
摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).
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