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    Method for obtaining a microbial strain for production of sphingoid bases
    23.
    发明授权
    Method for obtaining a microbial strain for production of sphingoid bases 有权
    获得用于生产类鞘氨醇碱的微生物菌株的方法

    公开(公告)号：US08372595B2

    公开(公告)日：2013-02-12

    申请号：US12300397

    申请日：2007-05-11

    申请人： Steffen Schaffer ,  Marco Alexander Van Den Berg ,  Daniel Boergel ,  Thomas Hueller

    发明人： Steffen Schaffer ,  Marco Alexander Van Den Berg ,  Daniel Boergel ,  Thomas Hueller

    IPC分类号： G01N33/53 ,  C12P7/18 ,  C12N1/00

    CPC分类号： C12N9/80 ,  A61K8/68 ,  A61K2800/86 ,  A61Q19/00 ,  C12N9/0071 ,  C12N9/1029

    摘要： The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity.

    摘要翻译： 本发明提供基因工程微生物菌株，特别是遗传工程酵母菌株，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。 本发明提供了一种获得基因工程的微生物菌株的方法，其产生至少0.5mg / g CDW的根据式I的鞘氨醇碱或其盐或酯。 该方法包括以下步骤：a）增加编码具有神经酰胺合成酶活性的酶的多核苷酸和/或具有神经酰胺酶活性的酶的表达，后者能够优先或甚至具体地水解含有类鞘氨醇碱的神经酰胺根据 式I，和/或b）减少编码具有鞘脂和Dgr.8-去饱和酶活性的酶的多核苷酸的表达和/或具有神经酰胺酶活性的酶，后者能够优先或甚至具体地水解含有植物鞘氨醇的神经酰胺或 二氢鞘氨醇作为鞘氨醇碱，并以所需的生产力分离菌株。

    FATTY ACID AND DERIVATIVES PRODUCTION
    26.
    发明申请
    FATTY ACID AND DERIVATIVES PRODUCTION 审中-公开
    脂肪酸和衍生物生产

    公开(公告)号：US20160138061A1

    公开(公告)日：2016-05-19

    申请号：US14943333

    申请日：2015-11-17

    申请人： Thomas HAAS ,  Markus Poetter ,  Steffen Schaffer

    发明人： Thomas HAAS ,  Markus Poetter ,  Steffen Schaffer

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6409 ,  Y02P20/59

    摘要： At least one fatty acid and/or derivative thereof is produced from a gas containing H2, CO2, and O2 by providing a genetically modified hydrogen oxidizing bacterium in an aqueous medium; and contacting the aqueous medium with the gas containing H2, CO2 and O2 in a weight ratio of 20 to 70 (H2): 10 to 45 (CO2): 5 to 35 (O2); wherein the fatty acid contains at least 5 carbon atoms and wherein the hydrogen oxidizing bacterium is genetically modified relative to the wild type bacterium to increase the expression of enzyme E1 that is capable of catalyzing the conversion of acetyl CoA to acyl ACP via malonyl coA and to increase the expression of enzyme E2 that is capable of catalyzing the conversion of Acyl ACP to the fatty acid.

    摘要翻译： 通过在水性介质中提供遗传修饰的氢氧化细菌，从含有H 2，CO 2和O 2的气体产生至少一种脂肪酸和/或其衍生物; 并使含水介质与含有H 2，CO 2和O 2的气体以20至70（H 2）：10至45（CO 2）：5至35（O 2）的重量比接触; 其中所述脂肪酸含有至少5个碳原子，并且其中所述氢氧化细菌相对于野生型细菌被遗传修饰以增加能够通过丙二酰辅酶A催化乙酰辅酶A转化为酰基ACP的酶E1的表达， 增加能够催化酰基ACP向脂肪酸转化的酶E2的表达。

    Microbial strains producing sphingoid bases or derivatives thereof
    27.
    发明授权
    Microbial strains producing sphingoid bases or derivatives thereof 有权
    产生螺旋体碱或其衍生物的微生物菌株

    公开(公告)号：US09234222B2

    公开(公告)日：2016-01-12

    申请号：US11666863

    申请日：2005-11-07

    申请人： Marco Alexander Van Den Berg ,  Steffen Schaffer

    发明人： Marco Alexander Van Den Berg ,  Steffen Schaffer

    IPC分类号： C12P13/00 ,  C12P13/02

    CPC分类号： C12P13/001 ,  C12P13/02

    摘要： The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide encoding an enzyme of the sphingolipid metabolic pathway. The present invention further provides a polypeptide having dihydroceramide desaturase activity obtainable form Pichia ciferrii.

    摘要翻译： 本发明提供了生产至少0.1mg / g生物量干重的类鞘氨醇碱的微生物菌株，特别是酵母菌株。 本发明还提供一种获得产生类鞘氨醇碱的微生物菌株的方法，包括在合适浓度的毒素存在下孵育一群微生物细胞，选择对所述毒素具有抗性的细胞，以及从毒素 - 产生至少0.1mg / g生物质干重的式I的类鞘氨醇碱。任选地，所述方法还包括使生产至少0.1mg / g生物量干重的毒素抗性微生物细胞群体 式I的鞘氨醇类碱基与编码鞘脂代谢途径酶的多核苷酸进行DNA介导的转化。 本发明还提供一种具有从毕赤酵母（Pichia ciferrii）获得的二氢神经酰胺去饱和酶活性的多肽。