公开(公告)号：US11046968B2

公开(公告)日：2021-06-29

申请号：US16006485

申请日：2018-06-12

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N15/82 ,  A23D9/00 ,  C12N9/12 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/10

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US10829794B2

公开(公告)日：2020-11-10

申请号：US16249253

申请日：2019-01-16

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  James Casey Lippmeier ,  David Simpson ,  Jun Wang ,  James P. Wynn ,  Ross Zirkle

IPC: C07H21/04 ,  C12P21/00 ,  C07K14/37 ,  C12N15/80 ,  C12R1/90 ,  C07K14/705 ,  C12N15/79 ,  C12N9/26

Abstract: The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

公开(公告)号：US10362794B2

公开(公告)日：2019-07-30

申请号：US15790584

申请日：2017-10-23

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  Joseph W. Pfeifer, III ,  Jon Milton Hansen ,  Paul Warren Behrens ,  Ross Zirkle ,  Tracey Lynn Stahl

IPC: A23L9/00 ,  A23K20/158 ,  A23D9/00 ,  A61K31/20 ,  C12P7/64 ,  C12R1/89 ,  A23K50/80 ,  A23L33/115 ,  A23L33/135 ,  A23K50/00 ,  A23L33/12 ,  A61K8/99 ,  A61K31/202 ,  A61K36/02 ,  A61Q19/00 ,  A61K35/00

Abstract: The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

公开(公告)号：US10272060B2

公开(公告)日：2019-04-30

申请号：US15889961

申请日：2018-02-06

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  Paul Warren Behrens ,  Jon Milton Hansen ,  Joseph W. Pfeifer, III ,  Tracey Lynn Stahl ,  Ross Zirkle

IPC: A23L2/52 ,  A61K8/36 ,  C12P7/64 ,  A23K50/80 ,  A23L33/00 ,  A23L33/12 ,  A61Q19/00 ,  A23K20/158 ,  A61K31/202

Abstract: The invention is directed to microbial oils containing omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid, eicosapentaenoic acid, and optionally docosapentaenoic acid and dosage forms containing such oils.

公开(公告)号：US20180282711A1

公开(公告)日：2018-10-04

申请号：US16006485

申请日：2018-06-12

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N9/10 ,  C12N15/82 ,  C12N9/12 ,  C11B1/04 ,  C11B1/00 ,  A23K20/158 ,  A23D9/00 ,  C11B1/10

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US10085465B2

公开(公告)日：2018-10-02

申请号：US15209568

申请日：2016-07-13

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. Apt ,  Joseph W. Pfeifer, III ,  Jon Milton Hansen ,  Paul Warren Behrens ,  Ross Zirkle ,  Tracey Lynn Stahl

IPC: C11C3/00 ,  A23K20/158 ,  A23D9/00 ,  A61K31/20 ,  C12P7/64 ,  C12R1/89 ,  A23K50/80 ,  A23L33/115 ,  A23L33/135 ,  A23K50/00 ,  A23L33/12 ,  A61K8/99 ,  A61K31/202 ,  A61K36/02 ,  A61Q19/00 ,  A61K35/00

Abstract: The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

公开(公告)号：US09994828B2

公开(公告)日：2018-06-12

申请号：US14555326

申请日：2014-11-26

Applicant: Dow AgroSciences LLC ,  DSM IP Assets B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N9/10 ,  C12N15/82 ,  A23D9/00 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  A23K20/158 ,  C12N9/12

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/1288 ,  C12N15/82 ,  C12N15/8247 ,  C12Y203/01085

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

公开(公告)号：US20170088841A1

公开(公告)日：2017-03-30

申请号：US15371410

申请日：2016-12-07

Applicant: DSM IP Assets B.V.

Inventor： Kirk E. APT ,  Leslie Richter ,  David Simpson ,  Ross Zirkle

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/04 ,  C12P7/64 ,  C12N9/10

CPC classification number: C12N15/52 ,  A61K38/00 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  C12Y101/01 ,  C12Y103/01 ,  C12Y203/01039 ,  C12Y203/01041 ,  C12Y402/01 ,  C12Y402/01059 ,  Y02P20/52

Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.

公开(公告)号：US20150299676A1

公开(公告)日：2015-10-22

申请号：US14555326

申请日：2014-11-26

Applicant: DOW AGROSCIENCES LLC ,  DSM IP ASSETS B.V.

Inventor： Terence A. Walsh ,  Daniel J. Gachotte ,  Cory M. Larsen ,  Scott Bevan ,  P. Ann Owens-Merlo ,  James G. Metz ,  Ross Zirkle

IPC: C12N9/10 ,  C11B1/10 ,  C11B1/04 ,  C11B1/00

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11B1/04 ,  C11B1/10 ,  C12N9/1288 ,  C12N15/82 ,  C12N15/8247 ,  C12Y203/01085

Abstract: This disclosure concerns recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

Abstract translation: 本公开涉及用多不饱和脂肪酸（PUFA）合酶系统遗传修饰的重组宿主生物体，以及允许和/或改善宿主生物体中PUFA生产的一种或多种辅助蛋白质。 本公开还涉及制造和使用这些生物以及从这些生物获得的产品的方法。

公开(公告)号：US20130143281A1

公开(公告)日：2013-06-06

申请号：US13633770

申请日：2012-10-02

Applicant: DSM IP Assets B.V.,

Inventor： Craig A. WEAVER ,  Ross Zirkle ,  Daniel H. Doherty ,  James G. Metz

IPC: C12P7/64

CPC classification number: C12P7/6427 ,  C12N9/93 ,  C12N15/52 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/62 ,  C12P7/625 ,  C12P7/6472

Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) olyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.

Abstract translation: 公开了嵌合多不饱和脂肪酸（PUFA）合成酮合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。