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    Multi-primer amplification method for barcoding of target nucleic acids
    21.
    发明授权
    Multi-primer amplification method for barcoding of target nucleic acids 有权

    公开(公告)号：US10344318B2

    公开(公告)日：2019-07-09

    申请号：US15590998

    申请日：2017-05-09

    Applicant: Fluidigm Corporation

    Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    IPC: C12Q1/686 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    NUCLEIC ACID ENCODING REACTIONS
    28.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开

    公开(公告)号：US20160208322A1

    公开(公告)日：2016-07-21

    申请号：US14723872

    申请日：2015-05-28

    Applicant: Fluidigm Corporation

    Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    29.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 审中-公开
    用于靶向靶核酸的多重PCR扩增方法

    公开(公告)号：US20140272952A1

    公开(公告)日：2014-09-18

    申请号：US14180262

    申请日：2014-02-13

    Applicant: Fluidigm Corporation

    Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    Abstract translation: 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

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