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    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
    21.
    发明授权
    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate 有权

    公开(公告)号：US09689006B2

    公开(公告)日：2017-06-27

    申请号：US14716767

    申请日：2015-05-19

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    IPC: C12P7/40 ,  C12P7/46 ,  C12N9/88

    CPC classification number: C12P7/46 ,  C12N9/88 ,  C12P7/40

    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    ALCOHOL DEHYDROGENASE VARIANTS
    22.
    发明申请
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
    酒精脱水变异

    公开(公告)号：US20160237410A1

    公开(公告)日：2016-08-18

    申请号：US15027169

    申请日：2014-10-03

    Applicant: GENOMATICA, INC.

    Inventor： Stefan Andrae ,  Michael Patrick Kuchinskas ,  Jingyi Li ,  Harish Nagarajan ,  Priti Pharkya

    IPC: C12N9/04 ,  C12P7/24

    Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    Abstract translation: 本文描述了与其未修饰的对应物相比，能够分别甲醇或乙醇转化为甲醛或乙醛的至少两倍的非天然NAD +依赖性醇脱氢酶（ADH）。 描述了编码非天然醇脱氢酶的核酸，以及包含核酸的表达构建体和包含核酸或表达构建体的工程化细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，用于促进生产的方法 的靶产物或其中间体，包括靶产物或中间体的组合物，以及由目标产物或中间体制成的产品。

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE
    24.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE 审中-公开
    芳香族化合物的2,4-二戊二酸和1,3-丁二烯的生物合成的微生物和方法

    公开(公告)号：US20150064750A1

    公开(公告)日：2015-03-05

    申请号：US14226631

    申请日：2014-03-26

    Applicant: Genomatica, Inc.

    Inventor： Robin E. OSTERHOUT ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

    IPC: C12P7/62 ,  C12P5/02

    CPC classification number: C12P7/62 ,  C12N15/52 ,  C12P5/005 ,  C12P5/026 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  Y02E50/10

    Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

    Abstract translation: 本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯途径。 本发明另外提供了使用这些生物体来生产甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯。

    METHANOL DEHYDROGENASE FUSION PROTEINS
    27.
    发明公开
    METHANOL DEHYDROGENASE FUSION PROTEINS 审中-公开

    公开(公告)号：US20230265397A1

    公开(公告)日：2023-08-24

    申请号：US17943482

    申请日：2022-09-13

    Applicant: Genomatica, Inc.

    Inventor： Nelson R. Barton ,  Jingyi Li ,  Joseph R. Warner ,  Priti Pharkya

    IPC: C12N9/04 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N9/10 ,  C12P7/24

    CPC classification number: C12N9/0006 ,  C12N9/88 ,  C12N9/90 ,  C12Y401/02043 ,  C12Y503/01027 ,  C12N15/52 ,  C12N9/1029 ,  C12P7/24 ,  C12Y101/02007 ,  C12Y203/01085 ,  C12Y401/02013 ,  C07K2319/00

    Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    MICROORGANISMS FOR PRODUCING 4C-5C COMPOUNDS WITH UNSATURATION AND METHODS RELATED THERETO
    30.
    发明申请
    MICROORGANISMS FOR PRODUCING 4C-5C COMPOUNDS WITH UNSATURATION AND METHODS RELATED THERETO 有权

    公开(公告)号：US20210002673A1

    公开(公告)日：2021-01-07

    申请号：US16888339

    申请日：2020-05-29

    Applicant: Genomatica, Inc.

    Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    IPC: C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12P7/40 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04

    Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

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