公开(公告)号：US20190204225A1

公开(公告)日：2019-07-04

申请号：US16295973

申请日：2019-03-07

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  B01L3/00 ,  G01N33/543

CPC classification number: G01N21/645 ,  B01L3/502707 ,  B01L3/502715 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/54373 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/061 ,  G01N2201/0642

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US10254225B2

公开(公告)日：2019-04-09

申请号：US15175489

申请日：2014-12-09

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/05 ,  G01N21/64 ,  B01L3/00 ,  G01N33/543

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. The biosensor also includes a shield layer having apertures that are positioned relative to the input regions of corresponding light guides such that the light emissions propagate through the apertures into the corresponding input regions. The shield layer extends between adjacent apertures and is configured to block the excitation light and the light emissions incident on the shield layer between the adjacent apertures.

公开(公告)号：US12151240B2

公开(公告)日：2024-11-26

申请号：US18154563

申请日：2023-01-13

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00 ,  B01J19/00 ,  B01L9/00 ,  C12Q1/6837

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US20240326042A1

公开(公告)日：2024-10-03

申请号：US18572786

申请日：2022-09-29

Applicant: ILLUMINA, INC.

Inventor： Bradley Drews ,  Darren Segale ,  Jennifer Foley ,  Jack Twomey

IPC: B01L3/00 ,  G01N15/1434

CPC classification number: B01L3/502715 ,  G01N15/1436 ,  B01L2200/026 ,  B01L2200/0689 ,  B01L2300/0887

Abstract: Flow cell manifold assemblies and methods are disclosed. In an implementation, an apparatus includes a flow cell and a flow cell manifold. The flow cell has a channel. The flow cell defines a plurality of first openings fluidically coupled to the channel and arranged on a first side of the channel and a plurality of second openings fluidically coupled the channel and arranged on a second side of the channel. The flow cell manifold assembly is coupled to the flow cell and has a first manifold fluidic line having a first fluidic line opening and being fluidically coupled to each of the first openings and has a second manifold fluidic line having a second fluidic line opening and being fluidically coupled to each of the second openings.

公开(公告)号：US12064761B2

公开(公告)日：2024-08-20

申请号：US16401805

申请日：2019-05-02

Applicant: Illumina, Inc.

Inventor： Cyril Delattre ,  Minsoung Rhee ,  Jeffrey Liu ,  Wesley Cox-Muranami ,  Paul Crivelli ,  Jennifer Foley ,  Darren Segale ,  Jay Taylor ,  Matthew Hage ,  Philip Paik ,  Erik Allegoren ,  David Hertzog ,  Alex Moroz-Smietana ,  Xiaoxiao Ma ,  Tsukasa Takahashi ,  Brandon Westerberg

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/16 ,  B01L2300/0887

Abstract: An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

公开(公告)号：US20230313275A1

公开(公告)日：2023-10-05

申请号：US18302193

申请日：2023-04-18

Applicant: Illumina, Inc.

Inventor： Darren Segale ,  Fiona E. Black ,  Jeffrey Dennis Brodin ,  Jeffrey Fisher ,  Lorenzo Berti ,  Siew Hong Leong

IPC: C12Q1/6816

CPC classification number: C12Q1/6816

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. In some embodiments, the barcode and the index are determined by sequencing.

公开(公告)号：US11181478B2

公开(公告)日：2021-11-23

申请号：US16295973

申请日：2019-03-07

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  H01L27/00 ,  G01N27/414 ,  G01N33/543 ,  G01N21/64 ,  B01L3/00 ,  H01L27/088 ,  C12Q1/6874 ,  H01L29/78 ,  G01N21/05

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.

公开(公告)号：US20210087613A1

公开(公告)日：2021-03-25

申请号：US17026069

申请日：2020-09-18

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Fiona E. Black ,  Jeffrey Dennis Brodin ,  Lorenzo Berti ,  Siew Hong Leong ,  Jeffrey S. Fisher ,  Allen Eckhardt ,  Rui Zhang ,  Yin Nah Teo

IPC: C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6874

Abstract: Some embodiments provided herein include methods and compositions for the detection of target ligands on an array. In some embodiments, a capture probe specifically binds to a target ligand from a sample, the location of a bead comprising the capture probe in an array is determined, and the bead is decoded to identify the capture probe and the sample. In some embodiments, a barcode is indicative of a capture probe attached to a bead; and an index is indicative of a subpopulation of beads. Some embodiments relate to sequencing target polynucleotides from several different nucleic acids samples on a bead array.

公开(公告)号：US10518263B2

公开(公告)日：2019-12-31

申请号：US16264146

申请日：2019-01-31

Applicant: Illumina, Inc.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: A61J1/06 ,  H01S4/00 ,  G01N35/00 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B01L3/00 ,  B01J19/00 ,  B01L9/00

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US20190240660A1

公开(公告)日：2019-08-08

申请号：US16264146

申请日：2019-01-31

Applicant: Illumina, Inc.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).