公开(公告)号：US20190352327A1

公开(公告)日：2019-11-21

申请号：US16411913

申请日：2019-05-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US20170022559A1

公开(公告)日：2017-01-26

申请号：US15207892

申请日：2016-07-12

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2525/113 ,  C12Q2535/122 ,  C12Q2563/107

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Abstract translation: 本公开提供了用于检测样品中多个不同核苷酸的方法和系统。 具体地，本公开提供了使用比检测到的核苷酸数目少于检测到的核苷酸数目和/或比检测到的核苷酸数目更少的成像事件来检测样品中的多个不同核苷酸。

公开(公告)号：US09273074B2

公开(公告)日：2016-03-01

申请号：US14512181

申请日：2014-10-10

Applicant: Illumina, Inc.

Inventor： Ryan Christopher Smith ,  Randall Smith ,  Xiaodong Zhao

IPC: C07F9/6503 ,  C07F9/6506 ,  A61K31/675 ,  C07F9/572 ,  C07H21/00

CPC classification number: C07F9/5728 ,  A61K31/675 ,  C07F9/65031 ,  C07F9/6506 ,  C07H21/00

Abstract: A compound has Formula I: A, B, C, D, W, X, Y, and Z are independently selected from hydrogen, optionally substituted C1-C6 alkyl, optionally substituted C1-C6 alkoxy, aryl, aldehyde, protected aldehyde, CH, N, O, S, null, and bond; Q is selected from aldehyde, protected aldehyde, and null, at least one of A, B, C, D, W, X, Y, Z, or Q is an aldehyde or protected aldehyde; the bonds between each of A-B, B-C, C-D, W-X, X-Y, and Y-Z are selected from single bond, double bond, triple bond, and no bond; L is a linker selected from a C1-C12 alkyl, aralkyl, and aryl, any of which is optionally substituted; one or more methylene unit (CH2) of the C1-C12 alkyl is optionally replaced by any combination of oxygen, carbonyl(C═O), and NH; and R1 and R2 are independently selected from —NR3R4, halogen, C1-C8 alkoxy, aralkoxy, alkenyloxy, alkynyloxy, and OCH2CH2CN; R3 and R4 are independently a C1-C4, straight chain or branched alkyl group.

Abstract translation: 化合物具有式I：A，B，C，D，W，X，Y和Z独立地选自氢，任选取代的C 1 -C 6烷基，任选取代的C 1 -C 6烷氧基，芳基，醛，被保护的醛，CH ，N，O，S，无效和键; Q选自醛，被保护的醛，无效，A，B，C，D，W，X，Y，Z或Q中的至少一个是醛或被保护的醛; A-B，B-C，C-D，W-X，X-Y和Y-Z各自之间的键选自单键，双键，三键和无键; L是选自C 1 -C 12烷基，芳烷基和芳基，其中任何一个任选被取代的连接体; C1-C12烷基的一个或多个亚甲基单元（CH2）任选被氧，羰基（C = O）和NH的任何组合取代; 并且R 1和R 2独立地选自-NR 3 R 4，卤素，C 1 -C 8烷氧基，芳烷氧基，烯氧基，炔氧基和OCH 2 CH 2 CN; R 3和R 4独立地为C 1 -C 4直链或支链烷基。

公开(公告)号：US20240400605A1

公开(公告)日：2024-12-05

申请号：US18806031

申请日：2024-08-15

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US20240390896A1

公开(公告)日：2024-11-28

申请号：US18790272

申请日：2024-07-31

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US12084474B2

公开(公告)日：2024-09-10

申请号：US16411913

申请日：2019-05-14

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C07H21/04 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2523/107 ,  C12Q2525/113 ,  C12Q2565/543

Abstract: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US20240271191A1

公开(公告)日：2024-08-15

申请号：US18611520

申请日：2024-03-20

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

CPC classification number: C12Q1/6837

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US11852586B2

公开(公告)日：2023-12-26

申请号：US17734953

申请日：2022-05-02

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

CPC classification number: G01N21/6428 ,  G01N21/6454 ,  G01N33/53 ,  G01N2021/6471 ,  G01N2201/08

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US20220220242A1

公开(公告)日：2022-07-14

申请号：US17565818

申请日：2021-12-30

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Randall Smith ,  Wayne George ,  Andrew Brown

IPC: C08F222/40 ,  C08F283/12 ,  C07H21/00

Abstract: In one example, an unsaturated cyclic dione is coupled to the substrate, and is reacted with an indole or indazole including a first functional group to form a first adduct coupling the first functional group to the substrate. In another example, an unsaturated cyclic dione is coupled to a substrate and reacted with a diene including a functional group to form an adduct coupling the functional group to the substrate. In another example, an indole or indazole is coupled to a substrate, and is reacted with an unsaturated cyclic dione including an oligonucleotide to form an adduct coupling the oligonucleotide to the substrate. In another example, a diene is coupled to a substrate, and is reacted with an unsaturated cyclic dione including an oligonucleotide to form an adduct coupling the oligonucleotide to the substrate.

公开(公告)号：US20220134333A1

公开(公告)日：2022-05-05

申请号：US17414612

申请日：2020-08-10

Applicant: ILLUMINA, INC.

Inventor： Steven Modiano ,  Dajun Yuan ,  Randall Smith

IPC: B01L3/00 ,  C08F220/56

Abstract: A method for patterning flow cell substrates using photo-initiated chemical reactions that includes fabricating a planar waveguide flow cell by forming a layer of light coupling gratings on a glass substrate layer; depositing a core layer on the layer of light coupling gratings; depositing a cladding layer on the core layer; and forming nanowells in the cladding layer; silanizing the cladding layer; coating the silanized cladding layer and nanowells with a first group of reactants; introducing a second group of reactants into the nanowells, wherein the second group of reactants includes a target reactant and a light-sensitive photoinitiator system; coupling a light source to the light coupling gratings and directing light internally within the planar waveguide flow cell for photo-initiating a chemical reaction between the first and second groups of reactants, wherein the photo-initiated chemical reaction covalently binds the target reactant to only the bottom portion of each nanowell.