公开(公告)号：US20090234090A1

公开(公告)日：2009-09-17

申请号：US12400417

申请日：2009-03-09

申请人： Toshihiro Ogawa ,  Kouji Tamori

发明人： Toshihiro Ogawa ,  Kouji Tamori

IPC分类号： C08F220/58

CPC分类号： C09D133/26 ,  C08K5/25

摘要： A coating agent for preventing nonspecific adsorption of a substance, which comprises a water-soluble copolymer (P) comprisinga repeating unit (A) represented by the following formula (1): wherein R0 represents a hydrogen atom or a methyl group and X represents a group represented by the following formula (1a): wherein R1 and R2 independently represent a hydrogen atom or unsubstituted or substituted an alkyl group having from 1 to 8 carbon atoms or formula (1b): wherein R3 and R4 independently represent a single bond, substituted or unsubstituted methylene or substituted or unsubstituted divalent alkylene group having from 2 to 7 carbon atoms wherein total of the number of carbon atoms of R3 and R4 is from 4 to 10, and Y represents any one of a single bond, O and S;a repeating unit (B) represented by the following formula (2): wherein R5 represents a hydrogen atom or a methyl group and Z represents an organic group containing at least one aldo group or keto group; anda hydrazide compound (H) comprising at least two hydrazino groups per one molecule.

摘要翻译： 一种用于防止物质非特异性吸附的涂层剂，其包含由下式（1）表示的重复单元（A）的水溶性共聚物（P）：其中R 0表示氢原子或甲基，X表示 由下式（1a）表示的基团：其中R1和R2独立地表示氢原子或未取代或取代的具有1至8个碳原子的烷基或式（1b）：其中R3和R4独立地表示单键， 取代或未取代的亚甲基或取代或未取代的2至7个碳原子的二价亚烷基，其中R3和R4的碳原子数合计为4-10，Y代表单键，O和S中任一个; 由下式（2）表示的重复单元（B）：其中R5表示氢原子或甲基，Z表示含有至少一个醛基或酮基的有机基团; 和每个分子包含至少两个肼基的酰肼化合物（H）。

公开(公告)号：US09051355B2

公开(公告)日：2015-06-09

申请号：US13636410

申请日：2011-03-23

申请人： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

发明人： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

IPC分类号： A61K38/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/286 ,  B01J20/288 ,  B01J20/32

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3219 ,  B01J20/3274 ,  C07K2317/10

摘要： Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. R—R2  (1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R2.

摘要翻译： 提供了具有优异的耐碱性的亲和色谱法的填充剂和分离免疫球蛋白的方法。 用于亲和层析的填料是其中由下式（1）表示的蛋白质固定在载体上的填料。 R-R2（1）其中R表示由4至300个氨基酸残基组成的氨基酸序列，其含有由4至20个连续组氨酸残基组成的区域; 并且R 2表示能够结合免疫球蛋白的氨基酸序列，所述氨基酸序列由含有蛋白A的Z结构域或其片段的50至500个氨基酸残基或其变体组成，条件是R与C-末端结合 或R2的N-末端。

公开(公告)号：US20140031522A1

公开(公告)日：2014-01-30

申请号：US13997015

申请日：2011-12-21

申请人： Shun-Cheng LI ,  Xing Li ,  Courtney Voss ,  Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani

发明人： Shun-Cheng LI ,  Xing Li ,  Courtney Voss ,  Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani

IPC分类号： C07K14/31

CPC分类号： C07K14/31 ,  B01D15/3809 ,  B01J20/28057 ,  B01J20/28078 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K17/06

摘要： The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.

摘要翻译： 本发明涉及具有耐碱特性的免疫球蛋白（Ig）结合蛋白。 在一个实施方案中，本发明提供Ig结合蛋白的变体，所述变体包含具有至少一个被组氨酸取代的天冬酰胺残基，丝氨酸，天冬氨酸或苏氨酸残基的Ig结合蛋白。 当与野生型Ig结合相比时，至少一种取代可赋予变体Ig结合蛋白在碱性溶液中增加的稳定性。 本发明还涉及用于亲和分离包含本发明的Ig结合蛋白的免疫球蛋白的基质，以及使用本发明的Ig结合蛋白质从混合物组合物中分离免疫球蛋白的方法。

公开(公告)号：US20140005357A1

公开(公告)日：2014-01-02

申请号：US13996978

申请日：2011-12-20

申请人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani ,  Shun-Cheng Li ,  Xing Li ,  Courtney Voss

发明人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani ,  Shun-Cheng Li ,  Xing Li ,  Courtney Voss

IPC分类号： C07K1/22

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/288 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3293 ,  C07K16/065 ,  C07K17/08

摘要： Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1): R—R2  (1) wherein R represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by ATK or ASK; and R2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which R2 binds to R is C-terminus or N-terminus of the immunoglobulin-binding domain.

摘要翻译： 提供了具有优异的耐碱性的亲和层析的支持物和分离免疫球蛋白的方法。 含有由下式（1）表示的固定化蛋白质配体的亲和层析的载体：R-R2（1）其中R表示由4至30个氨基酸残基组成的多肽，其含有由ATK或ASK表示的氨基酸序列 ; 并且R 2表示由50-500个氨基酸残基组成的多肽，其含有由SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列组成的免疫球蛋白结合结构域，其部分序列或具有 这些序列具有70％以上的同一性; 条件是R 2与R结合的末端是免疫球蛋白结合结构域的C末端或N末端。

公开(公告)号：US20130164761A1

公开(公告)日：2013-06-27

申请号：US13740763

申请日：2013-01-14

申请人： Masayuki TAKAHASHI ,  Tetsuo Fukuda ,  Kiyoshi Kasai ,  Toshihiro Ogawa ,  Satoshi Katayose ,  Kouji Tamori ,  Kinji Yamada

发明人： Masayuki TAKAHASHI ,  Tetsuo Fukuda ,  Kiyoshi Kasai ,  Toshihiro Ogawa ,  Satoshi Katayose ,  Kouji Tamori ,  Kinji Yamada

IPC分类号： G01N33/53

CPC分类号： C08G63/91 ,  B32B5/16 ,  G01N33/53 ,  G01N33/5434 ,  G01N33/54353 ,  Y10T428/2982 ,  Y10T428/2998

摘要： Carrier polymer particles comprising organic polymer particles having a particle diameter of 0.1 to 20 micrometers and a saccharide with which the surface of the organic polymer particles is covered, the organic polymer particles and the saccharide being chemically bonded.

摘要翻译： 包含粒径为0.1-20微米的有机聚合物颗粒的载体聚合物颗粒和被覆盖有机聚合物颗粒的表面的糖类，有机聚合物颗粒和化学键合的糖类。

公开(公告)号：US09162161B2

公开(公告)日：2015-10-20

申请号：US13638751

申请日：2011-03-29

申请人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Tomonori Shiotani ,  Kazuhiro Ikkyu ,  Yong Wang

发明人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Tomonori Shiotani ,  Kazuhiro Ikkyu ,  Yong Wang

IPC分类号： B01D15/04 ,  B01D15/08 ,  B01D15/38 ,  C08F8/00 ,  C08F220/26 ,  B01J20/26 ,  B01J20/286

CPC分类号： B01D15/3804 ,  B01J20/265 ,  B01J20/286 ,  C08F8/00 ,  C08F220/26 ,  Y10T428/2982

摘要： Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

摘要翻译： 本发明提供一种对蛋白质具有高动态结合能力并具有优异的耐碱性和储存稳定性的亲和层析用填料。 本发明的亲和层析用填料包含由40〜99.5质量份的含有甲基丙烯酰基的含有甲基丙烯酰基的乙烯基单体（不含有环氧基）的（M-1）共聚物构成的多孔质粒子 ，（M-2）含有环氧基的乙烯基单体0.5〜30质量份，除了单体（M）以外的（M-3）含有甲基丙烯酰基的乙烯基单体的（M-3） -1）和（M-2）和0〜25质量份（M-4）除了单体（M-1），（M-2）和（M-3） 条件是（M-1），（M-2），（M-3）和（M-4）的含量的总量为100质量份） 通过共聚物中包含的环氧基开环获得的开环环氧基; 和与多孔颗粒结合的配体。

公开(公告)号：US09090665B2

公开(公告)日：2015-07-28

申请号：US13638826

申请日：2011-03-29

申请人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Yong Wang

发明人： Kouji Tamori ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Hiroshi Kawai ,  Satoshi Hyugaji ,  Yong Wang

IPC分类号： G01N33/547 ,  C07K1/14 ,  G01N33/545 ,  C07K1/18 ,  B01D15/38 ,  C08F222/10 ,  B01J20/286 ,  C07K1/22

CPC分类号： C07K1/18 ,  B01D15/3804 ,  B01D15/3809 ,  B01J20/286 ,  C07K1/22 ,  C08F222/1006 ,  C08F220/20 ,  C08F2220/325 ,  C08F2220/281 ,  C08F220/58

摘要： Provided is a filler for affinity chromatography which is useful for protein purification and contains porous particles that have a high dynamic binding capacity for proteins and excellent pressure characteristics. The filler for affinity chromatography of the present invention is characterized in that it includes a porous particle consisting of a polymer of vinyl monomer including a cross-linkable vinyl monomer that contains hydroxyl group but does not contain epoxy group and an epoxy group-containing non-cross-linkable vinyl monomer, or a cross-linkable vinyl monomer that contains hydroxyl group and epoxy group, ligands bound to the porous particle, and ring-opened epoxy groups.

摘要翻译： 提供了用于亲和层析的填充剂，其用于蛋白质纯化，并且包含对于蛋白质具有高动态结合能力和优异的压力特性的多孔颗粒。 本发明的亲和层析用填料的特征在于，它包括由包含含有羟基但不含环氧基的可交联乙烯基单体的乙烯基单体的聚合物组成的多孔颗粒， 或可交联乙烯基单体，或含有羟基和环氧基的可交联乙烯基单体，与多孔颗粒结合的配体和开环的环氧基。

公开(公告)号：US07713627B2

公开(公告)日：2010-05-11

申请号：US11688653

申请日：2007-03-20

申请人： Kouji Tamori ,  Tetsuo Fukuta ,  Satoshi Katayose ,  Masaru Ueno ,  Mitsuhiro Murata

发明人： Kouji Tamori ,  Tetsuo Fukuta ,  Satoshi Katayose ,  Masaru Ueno ,  Mitsuhiro Murata

IPC分类号： B32B5/16

CPC分类号： G01N33/5434 ,  G01N33/54393 ,  Y10S525/921 ,  Y10T428/2991 ,  Y10T428/2998

摘要： Magnetic particles comprising a group shown by the following formula (1): wherein R1 and R2 individually represent a hydroxyl group, a group shown by the following formula (2), or a group shown by the following formula (3), provided that R1 and R2 are not both hydrogen atoms, wherein R3 represents an a linear, branched, or cyclic alkylene group having 2 to 6 carbon atoms or an arylene group, and wherein R4 is a hydrogen atom or an alkyl group.

摘要翻译： 包含由下式（1）表示的基团的磁性颗粒：其中R1和R2各自表示羟基，下式（2）表示的基团或下式（3）所示的基团，条件是R1 并且R 2不是氢原子，其中R 3表示具有2至6个碳原子的直链，支链或环状亚烷基或亚芳基，并且其中R4是氢原子或烷基。

公开(公告)号：US20080227893A1

公开(公告)日：2008-09-18

申请号：US10587220

申请日：2005-01-25

申请人： Kouji Tamori ,  Yasuhisa Watanabe ,  Akira Nishikawa

发明人： Kouji Tamori ,  Yasuhisa Watanabe ,  Akira Nishikawa

IPC分类号： C08L95/00 ,  C08J3/05

CPC分类号： G02B5/0242 ,  C08F265/02 ,  C08F265/04 ,  C08J3/11 ,  G02B5/0247 ,  G02B5/0278

摘要： A hollow particles-containing liquid composition which comprises:(A) crosslinked hollow particles having particle diameters of 0.4 μm or more and containing 40 mass % or more of a toluene insoluble, in an amount of 5 to 70 mass %,(B) a reactive diluent (B1) and/or an organic solvent (B2) in an amount of 95 to 30 mass % [the total mass of the crosslinked hollow particles (A) and the reactive diluent (B1) and/or the organic solvent (B2) is 100 mass %], and(C) a dispersing agent in an amount of 0 to 30 parts by mass relative to 100 parts by mass of the total of the crosslinked hollow particles (A) and the reactive diluent (B1) and/or the organic solvent (B2). There are provided a hollow particles-containing liquid composition which is low in foreign matter content and can give a high haze, a process for production thereof, and an optical article.

摘要翻译： 含有中空颗粒的液体组合物，其包含：（A）5〜70质量％的粒径为0.4μm以上且含有40质量％以上甲苯不溶性的交联中空粒子，（B）a 反应性稀释剂（B1）和/或有机溶剂（B2）的含量为95〜30质量％[交联中空粒子（A）和反应性稀释剂（B1）和/或有机溶剂（B2 ）为100质量％]，（C）相对于交联中空粒子（A）和反应性稀释剂（B1）的总量100质量份，0〜30质量份的分散剂和/ 或有机溶剂（B2）。 提供了含有异物含量低，可能产生高雾度的中空颗粒的液体组合物，其制造方法和光学制品。

公开(公告)号：US20050113505A1

公开(公告)日：2005-05-26

申请号：US10992816

申请日：2004-11-22

申请人： Yasuhisa Watanabe ,  Kouji Tamori

发明人： Yasuhisa Watanabe ,  Kouji Tamori

IPC分类号： C08F2/38 ,  C08F257/02 ,  C08F291/00 ,  C08J9/20 ,  C09D11/00 ,  C09D11/18 ,  C09D11/322 ,  C09D11/324 ,  C09D11/326 ,  C09D11/328 ,  C09D11/38 ,  G03G5/00

CPC分类号： C09D11/18 ,  C08F257/02 ,  C09D11/16 ,  C09D11/30 ,  C08F220/10

摘要： Disclosed are hollow polymer particles composed of a crosslinked polymer having a comparatively small particle diameter and a narrow particle diameter distribution, an aqueous dispersion of the particles, and an ink having good flying property. The hollow polymer particles are obtained by emulsion-polymerizing a crosslinkable polymerizable monomer composition absorbed in seed particles and containing a crosslinkable monomer component and a hydrophilic monomer component, wherein the seed particles are particles of a polymer having a weight average molecular weight of 500 to 15,000, and obtained by emulsion-polymerizing 100 parts by mass of a non-crosslinkable polymerizable monomer in the presence of 0.01 to 5 parts by mass of a chain transfer agent composed of at least one compound selected from the group consisting of α-methylstyrene dimer, thiuram disulfides and terpene hydrocarbons. The ink contains the hollow polymer particles.

摘要翻译： 公开了由具有较小粒径和窄粒径分布的交联聚合物，颗粒的水分散体和具有良好飞行性能的油墨组成的中空聚合物颗粒。 中空聚合物颗粒通过将吸附在种子颗粒中并含有可交联单体组分和亲水单体组分的可交联聚合单体组合物进行乳液聚合而获得，其中种子颗粒是重均分子量为500至15,000的聚合物颗粒 通过在0.01〜5质量份的由至少一种选自由α-甲基苯乙烯二聚体，甲基苯乙烯二聚物，甲基苯乙烯二聚物组成的组中选择的化合物的链转移剂的存在下，将100质量份的不可交联聚合性单体进行乳液聚合， 秋兰姆二硫化物和萜烯烃。 油墨含有中空聚合物颗粒。