公开(公告)号：US09937494B2

公开(公告)日：2018-04-10

申请号：US14739974

申请日：2015-06-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  Todd Roswech

IPC: B01L3/00 ,  B65D81/32 ,  B01F15/02 ,  G01N35/10 ,  B01F11/00

CPC classification number: B01L3/502 ,  B01F11/0008 ,  B01F11/0065 ,  B01F15/0238 ,  B01F15/028 ,  B01L3/505 ,  B01L3/527 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B65D81/3272 ,  G01N35/1002 ,  Y10T436/2575

Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.

公开(公告)号：US09901887B2

公开(公告)日：2018-02-27

申请号：US15282283

申请日：2016-09-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Thwar ,  Todd Roswech ,  John Andrew Sheridan

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  B01F3/08 ,  C12P19/34 ,  C12M1/26 ,  B01F15/00 ,  B04B11/02 ,  B01F5/04 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  B04B5/04 ,  B04B7/02 ,  C12Q1/68

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

公开(公告)号：US09776146B2

公开(公告)日：2017-10-03

申请号：US14696642

申请日：2015-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech

IPC: B01F5/06 ,  C12M1/34 ,  C12M3/00 ,  B01F3/08 ,  B01F5/04 ,  B01F13/00 ,  B04B5/04 ,  B04B7/02 ,  B01L3/00

CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

公开(公告)号：US20150314294A1

公开(公告)日：2015-11-05

申请号：US14799259

申请日：2015-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech ,  John Andrew Sheridan

IPC: B01L7/00 ,  B04B11/02 ,  B01F15/00

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。

公开(公告)号：US20150224457A1

公开(公告)日：2015-08-13

申请号：US14696642

申请日：2015-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech

IPC: B01F5/06

CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。