公开(公告)号：US20050186595A1

公开(公告)日：2005-08-25

申请号：US11016872

申请日：2004-12-21

申请人： Mie Ishii ,  Tomohiro Suzuki

发明人： Mie Ishii ,  Tomohiro Suzuki

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6816 ,  C12Q1/686 ,  C12Q2527/137

摘要： It is provided a nucleic acid labeling method that can reduce to a minimum the amount of a labeling substance used and can effectively incorporate the labeling substance into a product, a target nucleic acid to be detected. In the labeling of a target nucleic acid sequence in a product while it is amplified by PCR, at least one type from among four types of substrates A, T, C and G is chosen as a substrate to be labeled, the concentration of the chosen substrates is adjusted to be lower than that of the non-chosen substrates, and at least the labeled substrates are contained in the chosen substrates.

摘要翻译： 提供了一种核酸标记方法，其可以将所使用的标记物质的量减至最少，并且可以有效地将标记物质并入待检测的靶核酸的产物中。 在通过PCR扩增产物中标记产物中的靶核酸序列时，选择四种类型的底物A，T，C和G中的至少一种类型作为待标记的底物，选择的浓度 将衬底调整为低于未选择的衬底的衬底，并且至少标记的衬底被包含在所选择的衬底中。

公开(公告)号：US08792021B2

公开(公告)日：2014-07-29

申请号：US13255923

申请日：2010-03-31

申请人： Mie Ishii

发明人： Mie Ishii

IPC分类号： H04N9/64

CPC分类号： H04N5/3658

摘要： An image capturing apparatus includes an image sensor having an effective pixel area composed of effective pixels that photoelectrically convert an object image and a reference pixel area composed of reference pixels that output pixel signals to be a reference; a first calculation unit that calculates a standard deviation of the pixel signals output from the reference pixel area; a second calculation unit that calculates an integrated value of the pixel signals output from the reference pixel area; a third calculation unit that calculates a correction value for correcting pixel signals output from the effective pixel area, with use of the integrated value; a correction unit that corrects the pixel signals output from the effective pixel area, based on the correction value; and a determination unit that determines whether correction is to be performed by the correction unit, according to the standard deviation.

摘要翻译： 图像摄影装置包括：图像传感器，其具有由有效像素构成的有效像素区域，所述有效像素对所述物体图像进行光电转换;以及参考像素区域，所述参考像素区域由输出像素信号的参考像素构成; 第一计算单元，计算从参考像素区域输出的像素信号的标准偏差; 第二计算单元，计算从参考像素区域输出的像素信号的积分值; 第三计算单元，利用积分值计算用于校正从有效像素区域输出的像素信号的校正值; 校正单元，其基于校正值来校正从有效像素区域输出的像素信号; 以及确定单元，其根据标准偏差确定校正单元是否要执行校正。

公开(公告)号：US20120327280A1

公开(公告)日：2012-12-27

申请号：US13529252

申请日：2012-06-21

申请人： Mie Ishii

发明人： Mie Ishii

IPC分类号： H04N5/335

CPC分类号： H04N5/2176 ,  H01L27/3223 ,  H01L27/3269 ,  H04N5/347 ,  H04N5/361 ,  H04N5/3658 ,  H04N5/374 ,  H04N5/378

摘要： Provided is an imaging apparatus including an image sensor. In the image sensor that includes a valid pixel area in which a plurality of valid pixels each having a photoelectric conversion unit is disposed, a first reference pixel area in which a plurality of first reference pixels each having a light-shielded photoelectric conversion unit is disposed, and a second reference pixel area in which a plurality of second reference pixels each having no photoelectric conversion unit is disposed, when outputs of the plurality of valid pixels are added to be read, outputs of the plurality of first reference pixels are added by the addition unit to be read, and outputs of the plurality of second reference pixels are read without being added by the addition unit. Thus, noises can be effectively corrected even in the small number of invalid pixels areas.

摘要翻译： 提供了一种包括图像传感器的成像装置。 在包括其中设置有多个具有光电转换单元的有效像素的有效像素区域的图像传感器中，设置有各自具有遮光光电转换单元的多个第一参考像素的第一参考像素区域 以及第二参考像素区域，其中设置有多个不具有光电转换单元的第二参考像素，当多个有效像素的输出相加以被读取时，多个第一参考像素的输出被相加 读取的加法单元和多个第二参考像素的输出被读取，而不被加法单元添加。 因此，即使在少量的无效像素区域中也可以有效地校正噪声。

公开(公告)号：US07534566B2

公开(公告)日：2009-05-19

申请号：US11016872

申请日：2004-12-21

申请人： Mie Ishii ,  Tomohiro Suzuki

发明人： Mie Ishii ,  Tomohiro Suzuki

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

CPC分类号： C12Q1/6816 ,  C12Q1/686 ,  C12Q2527/137

摘要： It is provided a nucleic acid labeling method that can reduce to a minimum the amount of a labeling substance used and can effectively incorporate the labeling substance into a product, a target nucleic acid to be detected. In the labeling of a target nucleic acid sequence in a product while it is amplified by PCR, at least one type from among four types of substrates A, T, C and G is chosen as a substrate to be labeled, the concentration of the chosen substrates is adjusted to be lower than that of the non-chosen substrates, and at least the labeled substrates are contained in the chosen substrates.

摘要翻译： 提供了一种核酸标记方法，其可以将所使用的标记物质的量减至最少，并且可以有效地将标记物质并入待检测的靶核酸的产物中。 在通过PCR扩增产物中标记产物中的靶核酸序列时，选择四种类型的底物A，T，C和G中的至少一种类型作为待标记的底物，选择的浓度 将衬底调整为低于未选择的衬底的衬底，并且至少标记的衬底被包含在所选择的衬底中。

公开(公告)号：US20120249817A1

公开(公告)日：2012-10-04

申请号：US13515422

申请日：2010-12-03

申请人： Mie Ishii

发明人： Mie Ishii

IPC分类号： H04N5/335 ,  H04N5/225

CPC分类号： H04N9/045 ,  H04N5/335 ,  H04N5/3456 ,  H04N5/3532 ,  H04N5/3575 ,  H04N5/3696 ,  H04N5/37452

摘要： An image pickup apparatus that is capable of taking a distortion-free moving image without enlarging circuit scale. An image sensor has pixels each of which comprises a photoelectric conversion section that generates an electric charge corresponding to the incident light, a charge storage section that stores the electric charge, and an output section that outputs a voltage corresponding to the electric charge. The pixels include a first type pixel with a charge retention section that temporally holds the electric charge before transferring to the charge storage section, and a second type pixel without the charge retention section. A setting unit sets up a mode. A control unit controls to generate an image corresponding the output voltage from the first type pixel in a moving image pickup mode, and to generate an image corresponding to the output voltages from the first and second type pixels in a still image pickup mode.

摘要翻译： 能够在不放大电路规模的情况下拍摄无失真运动图像的摄像装置。 图像传感器具有像素，每个像素包括产生对应于入射光的电荷的光电转换部分，存储电荷的电荷存储部分和输出与电荷相对应的电压的输出部分。 像素包括具有电荷保留部分的第一类型像素，其在传送到电荷存储部分之前暂时保持电荷，以及第二类型像素，而没有电荷保持部分。 设置单元设置模式。 控制单元控制在运动图像拾取模式中产生对应于来自第一类型像素的输出电压的图像，并且在静止图像拾取模式中产生与来自第一和第二类型像素的输出电压相对应的图像。

公开(公告)号：US07964344B2

公开(公告)日：2011-06-21

申请号：US10939597

申请日：2004-09-14

申请人： Tomohiro Suzuki ,  Mie Ishii

发明人： Tomohiro Suzuki ,  Mie Ishii

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6832 ,  C12Q2565/501

摘要： A hybrid formed of a probe nucleic acid immobilized onto a substrate and a sample nucleic acid, in which the target strand of the sample nucleic acid is longer than the probe nucleic acid, so that at least a 5′ end portion of the target strand extends upstream the target sequence; a 3′ end of the target strand is the same as a 3′ end of the target sequence or extends downstream the target sequence by at least one base from the 3′ end of the target sequence; and provided that when the number of bases of the extending 5′ portion is designated as L1, and the number of bases of the extending 3′ portion of the target strand is designated as L2, a value of L1/L2 falls within the range of 0 to 1.5 both inclusive. This hybrid is very stable so that useful for detecting a small amount of nucleic acid specifically with high sensitivity.

摘要翻译： 由固定在底物上的探针核酸和样品核酸形成的杂交物，其中样品核酸的靶链长于探针核酸，使得目标链的至少5'末端延伸 上游目标序列; 目标链的3'末端与靶序列的3'末端相同或者在靶序列的下游延伸至目标序列的3'末端至少一个碱基; 并且当延伸的5'部分的基数被指定为L1，并且目标链的延伸3'部分的碱基数被指定为L2时，L1 / L2的值落在 0〜1.5 该杂交体非常稳定，因此可用于特异性高灵敏度检测少量核酸。

公开(公告)号：US20090011413A1

公开(公告)日：2009-01-08

申请号：US11808943

申请日：2007-06-14

申请人： Mie Ishii ,  Nobuko Yamamoto ,  Masahiro Kawaguchi ,  Tetsuo Okabe ,  Hiroki Sasaki ,  Satoshi Yajima ,  Yasuhiro Matsumura ,  Hisayuki Matsushita ,  Hiroyuki Tsunoda ,  Kunio Harada

发明人： Mie Ishii ,  Nobuko Yamamoto ,  Masahiro Kawaguchi ,  Tetsuo Okabe ,  Hiroki Sasaki ,  Satoshi Yajima ,  Yasuhiro Matsumura ,  Hisayuki Matsushita ,  Hiroyuki Tsunoda ,  Kunio Harada

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12N11/00

CPC分类号： C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/16

摘要： Colon cancer cells in a sample are screened by analyzing the amount of expression of at least 2 or more genes or products thereof selected from the group of genes listed in Tables 1 and 30. As compared to conventional method, patients having colon cancer can be detected with higher accuracy. Colon cancer cells in stool are also screened by analyzing expression of genes selected from the group of genes listed in Table 37.

摘要翻译： 通过分析选自表1和30中列出的基因组的至少2个或更多个基因或其产物的表达量来筛选样品中的结肠癌细胞。与常规方法相比，可以检测到具有结肠癌的患者 精度更高。 还通过分析表37所列基因的表达分析来筛选粪便中的结肠癌细胞。

公开(公告)号：US20060051788A1

公开(公告)日：2006-03-09

申请号：US11171234

申请日：2005-07-01

申请人： Tomohiro Suzuki ,  Mie Ishii

发明人： Tomohiro Suzuki ,  Mie Ishii

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6837 ,  C12Q2527/107 ,  C12Q2525/204

摘要： Provided is a probe set having the same level of hybrid stability when a plurality of probes are assigned to an identical nucleic acid to be detected. The probe set is adjusted in the binding strength, for example a Tm value, of each probe in consideration of the nucleotide length of single strand portions located on both sides of a hybrid composed of a nucleic acid to be detected and the probe. If necessary, a probe is assigned to a complementary strand of the nucleic acid to be detected. A probe substrate bound with the probe set is also provided.

摘要翻译： 提供了当将多个探针分配给待检测的相同核酸时，具有相同水平的杂交稳定性的探针组。 考虑到位于由待检测的核酸组成的杂交体和探针两侧的单链部分的核苷酸长度，探针组在每个探针的结合强度（例如Tm值）中进行调整。 如果需要，将探针分配给要检测的核酸的互补链。 还提供了与探针组结合的探针底物。

公开(公告)号：US20050214769A1

公开(公告)日：2005-09-29

申请号：US10519813

申请日：2003-06-27

申请人： Hiroyuki Hashimoto ,  Tadashi Okamoto ,  Mie Ishii

发明人： Hiroyuki Hashimoto ,  Tadashi Okamoto ,  Mie Ishii

IPC分类号： B01J19/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N37/00 ,  G06F19/00

CPC分类号： B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00693 ,  B01J2219/00707 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

摘要： To provide a technology for obtaining a probe carrier which is quality assured by an effective inspection program and an effective producing steps. Quality of a produced probe carrier is ensured by expanding test items in the processes from synthesis and purification of the probe to fixation thereof to a carrier.

摘要翻译： 提供用于获得通过有效检查程序和有效的制造步骤质量保证的探针载体的技术。 通过在从探针的合成和纯化到固定到载体的过程中扩大测试项目来确保产生的探针载体的质量。

公开(公告)号：US20050059069A1

公开(公告)日：2005-03-17

申请号：US10939597

申请日：2004-09-14

申请人： Tomohiro Suzuki ,  Mie Ishii

发明人： Tomohiro Suzuki ,  Mie Ishii

IPC分类号： C07H21/04 ,  C12M1/34 ,  C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q2565/501

摘要： A hybrid formed of a probe nucleic acid immobilized onto a substrate and a sample nucleic acid, in which the target strand of the sample nucleic acid is longer than the probe nucleic acid, so that at least a 5′ end portion of the target strand extends upstream the target sequence; a 3′ end of the target strand is the same as a 3′ end of the target sequence or extends downstream the target sequence by at least one base from the 3′ end of the target sequence; and provided that when the number of bases of the extending 5′ portion is designated as L1, and the number of bases of the extending 3′ portion of the target strand is designated as L2, a value of L1/L2 falls within the range of 0 to 1.5 both inclusive. This hybrid is very stable so that useful for detecting a small amount of nucleic acid specifically with high sensitivity.

摘要翻译： 由固定在底物上的探针核酸和样品核酸形成的杂交物，其中样品核酸的靶链长于探针核酸，使得目标链的至少5'末端延伸 上游目标序列; 目标链的3'末端与靶序列的3'末端相同或者在靶序列的下游延伸至目标序列的3'末端至少一个碱基; 并且当延伸的5'部分的基数被指定为L1，并且目标链的延伸3'部分的碱基数被指定为L2时，L1 / L2的值落在 0〜1.5 该杂交体非常稳定，因此可用于特异性高灵敏度检测少量核酸。