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    Gene editing through microfluidic delivery
    21.
    发明授权
    Gene editing through microfluidic delivery 有权

    公开(公告)号：US11125739B2

    公开(公告)日：2021-09-21

    申请号：US15542892

    申请日：2016-01-12

    申请人： Massachusetts Institute of Technology ,  President and Fellows of Harvard College

    发明人： Armon R. Sharei ,  Marc Lajoie ,  Klavs F. Jensen ,  Robert S. Langer

    IPC分类号： C12N15/87 ,  G01N33/50 ,  G01N33/487 ,  C12Q1/00

    摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.

    RESIDENCE STRUCTURES AND RELATED METHODS
    26.
    发明申请
    RESIDENCE STRUCTURES AND RELATED METHODS 有权

    公开(公告)号：US20210113460A1

    公开(公告)日：2021-04-22

    申请号：US17126786

    申请日：2020-12-18

    申请人： Massachusetts Institute of Technology ,  The Brigham and Women's Hospital, Inc.

    发明人： Andrew Bellinger ,  Shiyi Zhang ,  Carlo Giovanni Traverso ,  Robert S. Langer ,  Stacy Mo ,  Tyler Grant ,  Mousa Jafari ,  Dean Liang Glettig ,  Angela DiCiccio ,  Lowell L. Wood, JR. ,  Philip A. Eckhoff

    IPC分类号： A61K9/00 ,  A61K31/65 ,  A61K9/48 ,  A61M31/00 ,  A61K47/58 ,  A61K47/40 ,  A61K47/32 ,  C08G18/73 ,  C08G18/42 ,  A61K31/7048 ,  C08L33/14 ,  A61K47/34 ,  A61K31/357 ,  A61K47/42 ,  A61K47/69 ,  C08G83/00 ,  C08L33/02 ,  C08L33/08 ,  C08G63/08 ,  A61K47/10

    摘要： Residence structures, systems, and related methods are generally provided. Certain embodiments comprise administering (e.g., orally) a residence structure to a subject (e.g., a patient) such that the residence structure is retained at a location internal to the subject for a particular amount of time (e.g., at least about 24 hours) before being released. The residence structure may be, in some cases, a gastric residence structure. In some embodiments, the structures and systems described herein comprise one or more materials configured for high levels of active substances (e.g., a therapeutic agent) loading, high active substance and/or structure stability in acidic environments, mechanical flexibility and strength in an internal orifice (e.g., gastric cavity), easy passage through the GI tract until delivery to at a desired internal orifice (e.g., gastric cavity), and/or rapid dissolution/degradation in a physiological environment (e.g., intestinal environment) and/or in response to a chemical stimulant (e.g., ingestion of a solution that induces rapid dissolution/degradation). In certain embodiments, the structure has a modular design, combining a material configured for controlled release of therapeutic, diagnostic, and/or enhancement agents with a structural material necessary for gastric residence but configured for controlled and/or tunable degradation/dissolution to determine the time at which retention shape integrity is lost and the structure passes out of the gastric cavity. For example, in certain embodiments, the residence structure comprises a first elastic component, a second component configured to release an active substance (e.g., a therapeutic agent), and, optionally, a linker. In some such embodiments, the linker may be configured to degrade such that the residence structure breaks apart and is released from the location internally of the subject after a predetermined amount of time.