公开(公告)号：US06964747B2

公开(公告)日：2005-11-15

申请号：US10348123

申请日：2003-01-21

Applicant: Sukanta Banerjee ,  Cecilia Georgescu ,  Eric S. Daniels ,  Victoria L. Dimonie ,  Michael Seul

Inventor： Sukanta Banerjee ,  Cecilia Georgescu ,  Eric S. Daniels ,  Victoria L. Dimonie ,  Michael Seul

IPC: B01J13/02 ,  D06P3/52

CPC classification number: D06P1/90 ,  C08J3/215 ,  D06P1/908 ,  D06P1/92 ,  D06P1/922 ,  D06P1/924 ,  D06P1/926 ,  D06P1/928 ,  Y10T428/2987 ,  Y10T428/2989

Abstract: A dye, such as a fluorescent dye, is incorporated into polymer microparticles using a solvent system composed of a first solvent in which the dye and the microparticle polymer are soluble, a second solvent in which the dye and the microparticle polymer are not or only weakly soluble, and a third solvent in which the dye and the microparticle polymer are not or only weakly soluble. The first and second solvents are immiscible with each other, or at most partially miscible. The third solvent is miscible with the first and second solvents. The formulation provides substantially complete partitioning of the dye to the microparticles. The method may be used to obtain dyed polymer microparticle formed of cross-linked or non-cross-linked polymers. Libraries are provided comprising two or more sets of microparticles of different dye loadings. Fluorescent core-shell microparticles are produced from a mixture of microparticle cores incorporating one or more fluorescent dyes, a polymerization mixture comprising at least one polymerizable shell monomer, at least one free radical polymerization initiator comprising a water-insoluble oxidizing agent, and at least one water-soluble reducing agent.

Abstract translation: 使用由可溶于染料和微粒聚合物的第一溶剂构成的溶剂体系将染料，例如荧光染料引入聚合物微粒中，染料和微粒聚合物不是或仅仅是弱的第二溶剂 和其中染料和微粒聚合物不是或仅仅是弱溶解的第三溶剂。 第一和第二溶剂彼此不混溶，或最多部分混溶。 第三溶剂与第一溶剂和第二溶剂混溶。 该制剂提供染料基本上完全分配到微粒。 该方法可用于获得由交联或非交联聚合物形成的染色聚合物微粒。 提供包括两组或更多组不同染料负载的微粒子的文库。 荧光核壳微粒由掺入一种或多种荧光染料的微粒核心，包含至少一种可聚合壳单体的聚合混合物，至少一种包含水不溶性氧化剂的自由基聚合引发剂和至少一种 水溶性还原剂。

公开(公告)号：US20050246844A1

公开(公告)日：2005-11-10

申请号：US11179135

申请日：2005-07-12

Applicant: Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

Inventor： Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

IPC: C08J3/215 ,  A61K7/021 ,  A61K9/16 ,  A61K9/50

CPC classification number: C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

Abstract: Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.

Abstract translation: 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入到微粒中的溶质的量。 该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

公开(公告)号：US20050069956A1

公开(公告)日：2005-03-31

申请号：US10901864

申请日：2004-07-29

Applicant: Michael Seul

Inventor： Michael Seul

IPC: B01J19/00 ,  C07B61/00 ,  C40B40/06 ,  C40B40/10 ,  C40B70/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/567

CPC classification number: B01J19/0046 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B70/00

Abstract: Disclosed is a method for the physico-chemical encoding of a collection of beaded resin (“beads”) allowing determination of the chemical identity of bead-anchored compounds, following identification of beads bearing compounds of interest in an assay, by in-situ interrogation of individual beads, which does not require isolation of the beads of interest. These methods can be used to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial compounds libraries as well as multiplexed diagnostic and environmental testing aid other biochemical assays.

Abstract translation: 公开了一种用于物理化学编码珠粒树脂（“珠粒”）的集合的方法，其通过原位询问在鉴定在化验中带有感兴趣化合物的珠子之后，确定珠状锚定化合物的化学特性 的单个珠粒，其不需要分离感兴趣的珠粒。 这些方法可用于实现应用中的颜色编码策略，包括基于珠的组合化合物文库的超高通量筛选，以及多种诊断和环境测试辅助其他生物化学测定。

公开(公告)号：US20240273103A1

公开(公告)日：2024-08-15

申请号：US18440694

申请日：2024-02-13

Applicant: Michael Seul

Inventor： Michael Seul

IPC: G06F16/2455 ,  G06F16/2453 ,  G06F16/248

CPC classification number: G06F16/2456 ,  G06F16/24537 ,  G06F16/24544 ,  G06F16/248

Abstract: Disclosed is a method for querying by pattern matching a relational data store, including a collection of records having a preset number of attributes, wherein the representation of the content of a structured or unstructured data source also encodes the structure of the data source, and including these steps:



providing at least one query comprising at least a find- and a where-clause, wherein the where-clause comprises one or more query patterns, and the find-clause specifies a subset of attributes optionally including aliases thereof; executing the query by:

applying predicates in the one or more query patterns to identify a subset of matching or non-matching records;
forming the intersection of the two or more subsets of records for two or more patterns;
in accordance with the find-clause, selecting specified attributes and applying any aliases; and

thereby identifying the subset of records satisfying constraints expressed in the query patterns.

公开(公告)号：US09709559B2

公开(公告)日：2017-07-18

申请号：US10032657

申请日：2001-12-28

Applicant: Sukanta Banerjee ,  Michael Seul

Inventor： Sukanta Banerjee ,  Michael Seul

IPC: G01N33/543 ,  G01N33/58 ,  B01J19/00 ,  B82Y15/00

CPC classification number: G01N33/5434 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B82Y15/00 ,  C12Q1/6837 ,  C12Q1/6876 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54346 ,  G01N33/587 ,  G01N33/588

Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.

公开(公告)号：US09428799B2

公开(公告)日：2016-08-30

申请号：US13190147

申请日：2011-07-25

Applicant: Michael Seul

Inventor： Michael Seul

IPC: G01N33/48 ,  C12Q1/68 ,  G06F19/20

CPC classification number: C12Q1/6858 ,  G06F19/20 ,  C12Q2535/125 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2563/179

Abstract: A method of identifying alleles of polymorphic sites in a plurality of nucleic acid samples including the steps of determining a source tag sharing number “d” for each of the alleles; performing a first reaction in a plurality of pools of the alleles to be identified to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products to identify the alleles. If “d” is equal to or larger than a maximum pool size, the first reaction may not be performed. Alleles may be binned together. A microparticle comprising one or more capture probes each comprising an oligonucleotide complementary to a subsequence of a target polynucleotide.

Abstract translation: 一种鉴定多个核酸样品中多态位点的等位基因的方法，包括确定每个等位基因的源标签共有数“d”的步骤; 在要识别的等位基因的多个池中进行第一反应以产生包括识别所述每个池的源标签的反应产物; 集中池塘提供池池; 对于待鉴定的每个等位基因，使用所述反应产物进行第二反应以产生包含标记标签和衍生源标签的等位基因特异性第二反应产物; 识别所述等位基因特异性第二反应产物以鉴定等位基因。 如果“d”等于或大于最大池大小，则可能不执行第一反应。 等位基因可以合并在一起。 一种包含一种或多种捕获探针的微粒，每个捕获探针包含与靶多核苷酸的亚序列互补的寡核苷酸。

公开(公告)号：US09082145B2

公开(公告)日：2015-07-14

申请号：US14309850

申请日：2014-06-19

Applicant: Michael Seul

Inventor： Michael Seul

IPC: G01N33/46 ,  G06Q30/06 ,  G06F19/00 ,  G06Q30/00 ,  C40B20/04 ,  C40B50/10 ,  C40B50/16 ,  G06G7/48

CPC classification number: G06Q30/0631 ,  B01J2219/00576 ,  B01J2219/00592 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B20/04 ,  C40B50/10 ,  C40B50/16 ,  G06F19/34 ,  G06F19/3456 ,  G06Q30/018 ,  G06Q2220/10 ,  G16H10/60 ,  G16H50/30

Abstract: Genetic information relating to clinically significant attributes are generated as unique molecular signatures, which are provided to a subscriber to be carried as a card or in another form, including for display by a subscriber's computer or smart-phone or PDA. The signature is periodically updated as new clinically significant attributes become known. The process of updating the signature and using it to obtain suitable products (which don't have an unacceptable risk of generating an adverse reaction or outcome) is also described.

Abstract translation: 生成与临床重要属性相关的遗传信息，作为独特的分子标记产生，其被提供给用户作为卡或另一形式携带，包括由用户的计算机或智能手机或PDA显示。 当新的临床重要属性变得已知时，签名被定期更新。 还描述了更新签名并使用其获得合适产品（其不具有产生不利反应或结果的不可接受的风险）的过程。

公开(公告)号：US20130029857A1

公开(公告)日：2013-01-31

申请号：US13190147

申请日：2011-07-25

Applicant: Michael Seul

Inventor： Michael Seul

IPC: C40B30/04 ,  C40B30/10 ,  C07H21/00 ,  C40B30/00

CPC classification number: C12Q1/6858 ,  G06F19/20 ,  C12Q2535/125 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2563/179

Abstract: A method of identifying alleles of polymorphic sites in a plurality of nucleic acid samples including the steps of determining a source tag sharing number “d” for each of the alleles; performing a first reaction in a plurality of pools of the alleles to be identified to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products to identify the alleles. If “d” is equal to or larger than a maximum pool size, the first reaction may not be performed. Alleles may be binned together. A microparticle comprising one or more capture probes each comprising an oligonucleotide complementary to a subsequence of a target polynucleotide.

Abstract translation: 一种确定多个核酸样品中多态性位点的等位基因的方法，包括以下步骤：确定每个等位基因的源标签共享数d; 在要识别的等位基因的多个池中进行第一反应以产生包括识别所述每个池的源标签的反应产物; 集中池塘提供池池; 对于待鉴定的每个等位基因，使用所述反应产物进行第二反应以产生包含标记标签和衍生源标签的等位基因特异性第二反应产物; 识别所述等位基因特异性第二反应产物以鉴定等位基因。 如果d等于或大于最大池大小，则可能不执行第一反应。 等位基因可以合并在一起。 一种包含一种或多种捕获探针的微粒，每个捕获探针包含与靶多核苷酸的亚序列互补的寡核苷酸。

公开(公告)号：US20120203567A1

公开(公告)日：2012-08-09

申请号：US13366702

申请日：2012-02-06

Applicant: Michael Seul ,  Andreas E. Gocksch

Inventor： Michael Seul ,  Andreas E. Gocksch

IPC: G06Q50/22 ,  G06Q10/08

CPC classification number: G06Q50/22 ,  G06Q10/06315 ,  G06Q10/087

Abstract: Apparatuses, methods, and computer readable medium, for fulfilling a need for at least one perishable item, the method including reserving a plurality of perishable items with at least partially unknown attribute profiles from a supplier; receiving values for at least some of the reserved at least partially unknown attribute profiles, wherein the received values are determined by tests conducted after the reserving step; determining based on the received values which of the plurality of perishable items satisfy the need for the at least one perishable item; and if at least one of the plurality of perishable items does not satisfy the need for the at least one perishable item, unreserving the at least one perishable item of the plurality of perishable items determined not to satisfy the need for the at least one perishable item.

Abstract translation: 用于满足至少一个易腐物品的需要的装置，方法和计算机可读介质，所述方法包括从供应商保留具有至少部分未知属性简档的多个易腐物品; 接收所述保留的至少部分未知属性简档中的至少一些的值，其中通过在所述保留步骤之后进行的测试来确定所接收的值; 基于所接收的值确定所述多个易腐品中的哪一个满足对所述至少一个易腐物品的需要; 并且如果所述多个易腐物品中的至少一个不满足对所述至少一个易腐物品的需要，则不保留所述多个易腐物品中的至少一个易腐物品被确定为不满足所述至少一个易腐物品的需要 。

公开(公告)号：US08206953B2

公开(公告)日：2012-06-26

申请号：US11525064

申请日：2006-09-21

Applicant: Nataliya Korzheva ,  Michael Seul

Inventor： Nataliya Korzheva ,  Michael Seul

IPC: C12P19/34

CPC classification number: C12Q1/6837 ,  C12N15/1096 ,  C12Q1/6858 ,  C12Q1/6865 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2525/143 ,  C12Q2563/179 ,  C12Q2565/137 ,  C12Q2545/114 ,  C12Q2531/113

Abstract: Disclosed is a single stranded primer-promoter-selector construct comprising (in 3′ to 5′ orientation) a primer subsequence annealing to the target, a T7 or other promoter subsequence (the template strand), and a selector subsequence. The primer can be extended by template mediated elongation, including reverse transcription, or ligation to another oligonucleotide. The promoter sequence is oriented to direct the in-vitro transcription (IVT) opposite to that of primer extension, where the selector subsequence serves as a template for IVT. The selector is associated with the target subsequence of interest and it, and the amplified product are unique subsequences, dissimilar to other sequence present in the sample. The construct's is useful for determination of the presence and relative abundance of designated subsequences in the sample, multiplex gene expression analysis, multiplex allele counting, determination of polymorphic/mutation site, and loss of heterozygosity.

Abstract translation: 公开了一种单链引物 - 启动子选择构建体，其包含（靶向3'至5'取向）引物亚序列退火，T7或其它启动子亚序列（模板链）和选择子序列。 引物可通过模板介导的延伸扩增，包括逆转录或连接至另一寡核苷酸。 启动子序列被定向以引导与引物延伸相反的体外转录（IVT），其中选择子序列用作IVT的模板。 选择器与感兴趣的目标子序列相关联，并且扩增产物是与样本中存在的其他序列不同的唯一子序列。 该构建体可用于确定样品中指定的子序列的存在和相对丰度，多重基因表达分析，多重等位基因计数，多态/突变位点的确定和杂合性的丧失。