公开(公告)号：US20240301435A1

公开(公告)日：2024-09-12

申请号：US18609293

申请日：2024-03-19

Applicant: Monsanto Technology LLC

Inventor： Justin Arsenault ,  Yurong Chen ,  Byung-Guk Kang ,  Jennifer Kumpf ,  Brian J. Martinell ,  Lorena B. Moeller ,  Mary Ann Saltarikos ,  Ashok Shrawat ,  Shubha Subbarao ,  Edward J. Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00

CPC classification number: C12N15/8205 ,  A01H4/008

Abstract: The invention provides novel compositions and methods for improving the transformation of monocot seed excised embryo explants, which may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture, bud induction, extended bud induction, or regeneration of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explant with a heterologous polynucleotide by inoculating the embryo explant with a Rhizobiales bacterium comprising the heterologous polynucleotide. The methods provided herein also include methods of regenerating a genetically modified plant or plant part from a transformed or edited plant cell or explant.

公开(公告)号：US20240229063A1

公开(公告)日：2024-07-11

申请号：US18541484

申请日：2023-12-15

Applicant: Monsanto Technology LLC

Inventor： Matthew Bauer ,  Sarah Brown ,  Robert Thomas Gaeta ,  Michael Edward Goley ,  Shirley Guo ,  Erin Lyn Hall ,  Andrei Kouranov ,  Clayton Tyler Larue ,  Mingsheng Peng ,  Linda Rymarquis ,  Oscar Cornelius Sparks, JR. ,  Xudong Ye

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6895 ,  G01N33/569 ,  G01N33/573

CPC classification number: C12N15/8274 ,  C12N9/22 ,  C12N15/111 ,  C12N15/8213 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6895 ,  G01N33/56961 ,  G01N33/573 ,  C12N2310/20 ,  C12Q2600/13 ,  G01N2333/90206

Abstract: A transgenic corn event, Zm_CSM63715, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Zm_CSM63715 are also provided. Recombinant DNA molecules unique to the event Zm_CSM63715, and methods of using and detecting Zm_CSM63715 are also provided. Corn plants containing the event Zm_CSM63715 exhibit tolerance to PPO inhibitors.

公开(公告)号：US20240117366A1

公开(公告)日：2024-04-11

申请号：US18481464

申请日：2023-10-05

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C12N9/10 ,  G01N33/00

CPC classification number: C12N15/8209 ,  A01H4/003 ,  A01H4/008 ,  C12N5/0025 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8201 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8221 ,  C12N15/8265 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  G01N33/0098 ,  A01H6/202 ,  C12N15/8261

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20230159944A1

公开(公告)日：2023-05-25

申请号：US17820172

申请日：2022-08-16

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8214 ,  C12N9/22

Abstract: Provided are methods for plastid genome editing and development of plants, plant cells, plant parts, and seeds comprising edited plastid genomes. Compositions for transformation of plastid genomes are further provided. Specifically, the plastid genome is modified to comprise the replacement of a targeted endogenous plastid genome sequence with a modified version of the target plastid sequence, where the modified plastid genome sequence has been designed to deliberately reduce its homology to the targeted endogenous plastid genome sequence and, in some cases, encode a protein containing one or more mutations.

公开(公告)号：US11485980B2

公开(公告)日：2022-11-01

申请号：US16904347

申请日：2020-06-17

Applicant: Monsanto Technology LLC

Inventor： Erik Dersch ,  Richard J. Heinzen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Xudong Ye

IPC: C12N15/82 ,  C12N5/00 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10 ,  A01H6/20 ,  A01H6/54 ,  A01H6/46 ,  A01H6/60 ,  C12N1/20 ,  C12R1/41

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

公开(公告)号：US10752908B2

公开(公告)日：2020-08-25

申请号：US16242887

申请日：2019-01-08

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/84 ,  C12R1/41 ,  C12N15/82

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

公开(公告)号：US10717983B2

公开(公告)日：2020-07-21

申请号：US15826540

申请日：2017-11-29

Applicant: Monsanto Technology, LLC

Inventor： Erik Dersch ,  Richard J. Heinzen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

公开(公告)号：US20190382777A1

公开(公告)日：2019-12-19

申请号：US16393833

申请日：2019-04-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US10202611B2

公开(公告)日：2019-02-12

申请号：US14642674

申请日：2015-03-09

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Michael W. Petersen

IPC: C12N15/84 ,  C12N15/82

Abstract: Methods and compositions for improved bacterial-mediated plant transformation are provided. The methods generally allow plant transformation with reduced vector backbone integration and a high frequency of low-copy transformation events. Vectors for achieving these results are described, as are methods for their use.

公开(公告)号：US20180312854A1

公开(公告)日：2018-11-01

申请号：US15977229

申请日：2018-05-11

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/82 ,  G01N33/50 ,  C12R1/41

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.