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21.发明授权Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation 失效通过荧光共振能量转移标记产生检测核苷酸序列变异公开(公告)号：US06573047B1
公开(公告)日：2003-06-03
申请号：US09547292
申请日：2000-04-11
IPC分类号： C12Q168
CPC分类号： C12Q1/6858 , C12Q2565/101 , C12Q2535/125
摘要： The present invention provides methods and kits for identifying nucleotides at a variant site in a target molecule by forming fluorescence resonance energy transfer labeled product. The location and type of fluorescent labels in the labeled product provide strong signals and relatively high spectral purity that facilitate detection. Utilizing various secondary labels and different combinations of acceptor and donor labels, certain methods permit multiple analyses to be conducted simultaneously and at high throughput. The methods can be used in a variety of applications such as analyzing point mutations and single nucleotide polymorphisms (SNPs). In addition, the methods have utility in other applications in which specific sequence information is of value, including detection of pathogens, paternity disputes, prenatal testing and forensic analysis.
摘要翻译： 本发明提供用于通过形成荧光共振能量转移标记产物来鉴定靶分子中变体位点处的核苷酸的方法和试剂盒。 标记产品中荧光标记的位置和类型提供强信号和较高的光谱纯度，便于检测。 利用各种二级标签和受体和供体标签的不同组合，某些方法允许同时进行多次分析并以高产量进行。 该方法可用于各种应用，如分析点突变和单核苷酸多态性（SNPs）。 此外，这些方法在其他应用中具有实用价值，其中具体的序列信息是有价值的，包括病原体的检测，亲子鉴定，产前检测和法医分析。
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22.发明授权Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels 有权微型毛细管电泳芯片及同时检测多种氧化还原标记物的方法公开(公告)号：US06361671B1
公开(公告)日：2002-03-26
申请号：US09229386
申请日：1999-01-11
IPC分类号： G01N2726
CPC分类号： G01N27/4473
摘要： This invention relates to a microfabricated capillary electrophoresis chip for detecting multiple redox-active labels simultaneously using a matrix coding scheme and to a method of selectively labeling analytes for simultaneous electrochemical detection of multiple label-analyte conjugates after electrophoretic or chromatographic separation.
摘要翻译： 本发明涉及一种用于同时使用基质编码方案检测多种氧化还原活性标记物的微细毛细管电泳芯片，以及选择性标记用于电泳或色谱分离后多种标记 - 分析物共轭物的电化学检测的分析物的方法。
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23.发明授权Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores 失效二聚荧光能量转移染料包括不对称花青唑 - 吲哚宁发色团
公开(公告)号：US5929227A
公开(公告)日：1999-07-27
申请号：US965913
申请日：1997-11-07
IPC分类号： G01N27/447 , C07H21/02 , C07H21/04
CPC分类号： G01N27/44721
摘要： Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.
摘要翻译： 提供新型荧光异二聚体DNA染色能量转移染料，其结合不对称花青唑 - 吲哚宁染料，其提供强的DNA亲和力，大的斯托克斯位移和在红色区域中的发射。 这些染料特别适用于凝胶电泳和可能在多种情况下与各种组合物结合的标记物。 提供了试剂盒和各种化合物，其中试剂盒可用于同时检测各种部分，特别是使用单个窄波长照射源。 单个化合物的特征在于由于优化分离两个发色团的连接基团的长度而导致高的供体猝灭和对dsDNA的高亲和力。
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24.发明授权Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores 失效二聚荧光能量转移染料包括不对称花青唑 - 吲哚宁发色团
公开(公告)号：US5565554A
公开(公告)日：1996-10-15
申请号：US283006
申请日：1994-07-29
IPC分类号： C07D417/14 , C09B23/02 , G01N27/447 , C07H19/00 , C07D221/12
CPC分类号： C07D417/14 , C09B23/02 , G01N27/44726
摘要： Novel fluorescent DNA-staining dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts.
摘要翻译： 提供新型荧光DNA染色染料，结合不对称花青唑 - 吲哚宁染料，提供强的DNA亲和力，大的斯托克斯位移和光谱红色区域的发射。 这些染料特别适用于凝胶电泳和可能在多种情况下与各种组合物结合的标记物。
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25.发明授权Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display 有权多功能重组藻胆蛋白基荧光结构和phycobilisome显示公开(公告)号：US07169582B2
公开(公告)日：2007-01-30
申请号：US10617208
申请日：2003-07-10
申请人： Alexander N. Glazer , Yuping Cai
发明人： Alexander N. Glazer , Yuping Cai
CPC分类号： C07K14/415 , C07K2319/00 , Y10S435/822
摘要： The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
摘要翻译： 本发明提供了多功能融合构建体，其迅速并入大分子结构例如藻糖酵母中，使得融合蛋白彼此分离并且不能自相关。 本发明提供了用于在寡聚藻胆蛋白上显示功能性多肽结构域的方法和组合物，包括融合蛋白，其包含功能性显示结构域和掺入功能性寡聚藻胆蛋白的功能性藻胆蛋白结构域。 融合蛋白提供新的特异性标记试剂。
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26.发明授权公开(公告)号：US06280933B1
公开(公告)日：2001-08-28
申请号：US08966398
申请日：1997-11-07
IPC分类号： C12Q168
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333 , C12Q2565/102 , C12Q2563/173
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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公开(公告)号：US6054272A
公开(公告)日：2000-04-25
申请号：US965917
申请日：1997-11-07
IPC分类号： C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/68 , G01N27/447 , C07H21/04
CPC分类号： C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747
摘要： Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
摘要翻译： 提供了新的荧光染料，其特征在于具有连接到阳离子链的荧光团。 与没有聚阳离子链的荧光团相比，发现这些染料提供与核酸结合的高度增强并且与核酸具有强的结合亲和力。 该染料可用于使用基本上较少染料的凝胶电泳和溶液中以显着更高的灵敏度检测dsDNA。
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28.发明授权Electrochemical detector integrated on microfabricated capillary electrophoresis chips 失效电化学检测器集成在微细毛细管电泳芯片上
公开(公告)号：US5906723A
公开(公告)日：1999-05-25
申请号：US703394
申请日：1996-08-26
IPC分类号： G01N27/447 , G01N30/60 , G01N27/26
CPC分类号： G01N27/44791 , G01N27/4473 , G01N30/6095
摘要： A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.
摘要翻译： 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。
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29.发明授权Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels 失效在能量转移标签中具有高吸光度横截面作为供体发色团的花青染料
公开(公告)号：US5853992A
公开(公告)日：1998-12-29
申请号：US726178
申请日：1996-10-04
CPC分类号： C12Q1/6818 , C12Q1/6869
摘要： Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
摘要翻译： 能量转移标记中使用花青染料作为供体荧光团，其中光能被供体荧光团吸收并转移到受体荧光团，受体荧光基团通过发射荧光检测反应。 花青染料赋予标签异常高的灵敏度，从而改善其在各种生物化学方法，特别是核酸测序，核酸片段大小和相关方法中的用途。
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30.发明授权
公开(公告)号：US5760201A
公开(公告)日：1998-06-02
申请号：US500798
申请日：1995-07-12
IPC分类号： C07D417/14 , C09B23/02 , G01N27/447 , C07H21/02 , C12Q1/68
CPC分类号： C07D417/14 , C09B23/02 , G01N27/44726
摘要： Novel fluorescent heterodimeric DNA-staining energy transfer dyes are provided combining asymmetric cyanine azole-indolenine dyes, which provide for strong DNA affinity, large Stokes shifts and emission in the red region of the spectrum. The dyes find particular application in gel electrophoresis and for labels which may be bound to a variety of compositions in a variety of contexts. Kits and individual compounds are provided, where the kits find use for simultaneous detection of a variety of moieties, particularly using a single narrow wavelength irradiation source. The individual compounds are characterized by high donor quenching and high affinity to dsDNA as a result of optimizing the length of the linking group separating the two chromophores.
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