公开(公告)号：US11795212B2

公开(公告)日：2023-10-24

申请号：US17093781

申请日：2020-11-10

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin Rottman ,  Alexander L. Watters

IPC: C07K16/12 ,  A61K47/68 ,  G01N33/543 ,  G01N33/569 ,  C07K14/42

CPC classification number: C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  C07K2319/33 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/30

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：US20230146985A1

公开(公告)日：2023-05-11

申请号：US18146483

申请日：2022-12-27

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Je-Hyuk Lee ,  George M. Church ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/53

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

公开(公告)号：US11293052B2

公开(公告)日：2022-04-05

申请号：US17238682

申请日：2021-04-23

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Jehyuk Lee ,  Daniel Levner ,  Michael Super

IPC: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12P19/34

Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.

公开(公告)号：US20220025448A1

公开(公告)日：2022-01-27

申请号：US17498829

申请日：2021-10-12

Applicant: President and Fellows of Harvard College

Inventor： Daniel Levner ,  Je-Hyuk Lee ,  George M. Church ,  Michael Super

IPC: C12Q1/6837 ,  C12Q1/6804 ,  C12Q1/6816

Abstract: The inventions provided herein relate to detection reagents, compositions, methods, and kits comprising the detection reagents for use in detection, identification, and/or quantification of analytes in a sample. Such detection reagents and methods described herein allow multiplexing of many more labeled species in the same procedure than conventional methods, in which multiplexing is limited by the number of available and practically usable colors.

公开(公告)号：US11059874B2

公开(公告)日：2021-07-13

申请号：US16892577

申请日：2020-06-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Jeffrey Charles Way ,  Donald E. Ingber

IPC: C07K14/47 ,  G01N33/569 ,  C07K16/44 ,  C07K17/00 ,  G01N33/50 ,  G01N27/74

Abstract: The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.

公开(公告)号：US10753940B2

公开(公告)日：2020-08-25

申请号：US15749976

申请日：2016-08-03

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Olivier Y. F. Henry ,  Michael Super

IPC: G01N33/58 ,  G01F1/64 ,  G01N33/543 ,  C12Q1/68 ,  C12Q1/6816 ,  G01N33/535 ,  B82Y30/00

Abstract: The invention described herein relates generally to methods, sensors, devices and kits for electrochemical detection of a target analyte in a sample. In certain aspects, the methods, sensors, devices and kits described herein can be used to detect low concentrations of at least one target analyte using small sample volumes. In some embodiments, methods, sensors and kits for detecting a microbe, microbe fragment or released endotoxin in a test sample, including bodily fluids such as blood and tissues of a subject, food, water, and environmental surfaces, are also provided herein.

公开(公告)号：US10718766B2

公开(公告)日：2020-07-21

申请号：US15668794

申请日：2017-08-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Mark J. Cartwright ,  Nazita Gamini ,  Donald E. Ingber ,  Martin Rottman ,  Michael Super ,  Julie A. Tomolonis ,  Karen A. Sinclair

IPC: A61K39/00 ,  A61K39/02 ,  C12Q1/00 ,  G01N33/569 ,  C12Q1/18

Abstract: The disclosure provides methods, compositions, and kits for enhanced detection of microbes in samples and monitoring of antimicrobial activity in a subject.

公开(公告)号：US10550272B2

公开(公告)日：2020-02-04

申请号：US15944619

申请日：2018-04-03

Applicant: President and Fellows of Harvard College

Inventor： Joanna Aizenberg ,  Benjamin Hatton ,  Donald Ingber ,  Michael Super ,  Tak Sing Wong

IPC: C09D5/16 ,  A61L15/24 ,  A61L15/34 ,  A61L15/42 ,  A61L15/46 ,  A61L27/28 ,  A61L27/34 ,  A61L27/50 ,  A61L33/00 ,  A61L33/06 ,  F15D1/02 ,  F15D1/10 ,  B05D5/08

Abstract: A self-healing, scratch resistant slippery surface that is manufactured by wicking a chemically-inert, high-density liquid coating over a roughened solid surface featuring micro and nanoscale topographies is described. Such a slippery surface shows anti-wetting properties, as well as exhibits significant reduction of adhesion of a broad range of biological materials, including particles in suspension or solution. Specifically, the slippery surfaces can be applied to medical devices and equipment to effectively repel biological materials such as blood, and prevent, reduce, or delay coagulation and surface-mediated clot formation. Moreover, the slippery surfaces can be used to prevent fouling by microorganisms such as bacteria.

公开(公告)号：US10435457B2

公开(公告)日：2019-10-08

申请号：US15750788

申请日：2016-08-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  C07K14/00 ,  C07K19/00 ,  G01N33/569 ,  C07K14/47 ,  A61K38/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e.g., enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US20190077850A1

公开(公告)日：2019-03-14

申请号：US16059799

申请日：2018-08-09

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Michael Super ,  Alexander Watters

IPC: C07K16/12 ,  G01N33/487 ,  G01N33/569 ,  G01N33/543 ,  A61P31/04

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. The microbe-targeting or microbe-binding molecules can comprise a microbe surface-binding domain linked to a portion of an Fc region. Further, the microbe-targeting molecules can be conjugated to substrate (e.g., a magnetic particle) to form a microbe-targeting substrate. Such microbe-targeting molecules and/or substrates and the kits comprising the same can be used in various applications, such as diagnosis and/or treatment of an infection caused by microbes. Moreover, the microbe-targeting molecules and/or substrates can be easily regenerated after use.