公开(公告)号：US10041950B2

公开(公告)日：2018-08-07

申请号：US13849160

申请日：2013-03-22

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder, II ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/682 ,  G01N33/542

CPC分类号： G01N33/581 ,  C12Q1/68 ,  C12Q1/682 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US11959838B2

公开(公告)日：2024-04-16

申请号：US15965786

申请日：2018-04-27

申请人： Ventana Medical Systems, Inc.

发明人： Nelson Alexander ,  Aoune Barhoumi ,  Melinda Day ,  Lisa Gallegos ,  Katherine Leith ,  Samantha Rajkovich ,  Esteban Roberts ,  Stacey Stanislaw ,  Eric Walk

IPC分类号： G01N1/28 ,  A01N1/02 ,  G01N1/30 ,  G01N1/36 ,  G01N33/483

CPC分类号： G01N1/286 ,  A01N1/0226 ,  A01N1/0278 ,  G01N1/30 ,  G01N1/36 ,  G01N33/4833 ,  G01N2001/2866 ,  G01N2001/368

摘要： The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially “personalized” anti-tumor vaccines or immune cell based therapies.

公开(公告)号：US20220178934A1

公开(公告)日：2022-06-09

申请号：US17678389

申请日：2022-02-23

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder, II ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： G01N33/58 ,  C12Q1/682 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US20210394172A1

公开(公告)日：2021-12-23

申请号：US17359683

申请日：2021-06-28

申请人： Ventana Medical Systems, Inc.

发明人： Nathan Crum ,  Timothy Keller ,  Alastair Laing ,  Michael Otter ,  Vinnie Petre ,  Elizabeth Pigman ,  Taylor Shingler ,  Stacey Stanislaw ,  Benjamin Stevens

IPC分类号： B01L3/00 ,  G01N1/31 ,  B01L9/06 ,  G01N35/10

摘要： A tissue sample that has been removed from a subject can be properly fixed for evaluation using the disclosed transporter assembly for carrying a tissue sample and method for fixing an unfixed tissue sample. In one embodiment, the disclosed assembly includes a transport container, a fixative in the transport container, and a cooling device that reduces and/or maintains the temperature of the fixative to perform a pre-soaking process at a temperature of less than about 7° C. The pre-soaking process can, for example, be performed during sample transport or during extended periods of storage, such as over a weekend.

公开(公告)号：US20200333349A1

公开(公告)日：2020-10-22

申请号：US16856604

申请日：2020-04-23

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： G01N33/58 ,  C12Q1/682 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US10557851B2

公开(公告)日：2020-02-11

申请号：US16038389

申请日：2018-07-18

申请人： VENTANA MEDICAL SYSTEMS, INC.

发明人： Nelson Alexander ,  William Day ,  Jerome W. Kosmeder, II ,  Mark Lefever ,  Larry Morrison ,  Anne M. Pedata ,  Stacey Stanislaw

IPC分类号： C12Q1/68 ,  G01N33/58 ,  C12Q1/682 ,  G01N33/53 ,  G01N33/542

摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

公开(公告)号：US20170320054A1

公开(公告)日：2017-11-09

申请号：US15659581

申请日：2017-07-25

申请人： Ventana Medical Systems, Inc.

发明人： Nathan Crum ,  Timothy Keller ,  Alastair Laing ,  Michael Otter ,  Vinnie Petre ,  Elizabeth Pigman ,  Taylor Shingler ,  Stacey Stanislaw ,  Benjamin Stevens

IPC分类号： B01L3/00 ,  G01N35/10

CPC分类号： B01L3/502 ,  B01L3/50825 ,  B01L7/04 ,  B01L9/06 ,  B01L2200/026 ,  B01L2200/147 ,  B01L2200/18 ,  B01L2300/024 ,  B01L2300/047 ,  B01L2300/0609 ,  B01L2300/1855 ,  B01L2300/1877 ,  B01L2300/1883 ,  B01L2300/1894 ,  G01N1/31 ,  G01N35/10 ,  G01N2001/315

摘要： A tissue sample that has been removed from a subject can be properly fixed for evaluation using the disclosed transporter assembly for carrying a tissue sample and method for fixing an unfixed tissue sample. In one embodiment, the disclosed assembly includes a transport container, a fixative in the transport container, and a cooling device that reduces and/or maintains the temperature of the fixative to perform a pre-soaking process at a temperature of less than about 7° C. The pre-soaking process can, for example, be performed during sample transport or during extended periods of storage, such as over a weekend.

公开(公告)号：US20130196880A1

公开(公告)日：2013-08-01

申请号：US13746455

申请日：2013-01-22

申请人： Ventana Medical Systems, Inc.

发明人： Stacey Stanislaw ,  Heather Lewis ,  Lei Tang ,  Lizhen Pang ,  Hong Ni

IPC分类号： C40B40/06 ,  C40B40/12 ,  C40B40/10 ,  C12Q1/68 ,  G01N33/68

CPC分类号： C40B40/06 ,  C12Q1/6837 ,  C40B40/10 ,  C40B40/12 ,  G01N21/253 ,  G01N33/54366 ,  G01N33/6803 ,  C12Q2565/513

摘要： Disclosed herein are devices for the detection of target molecules and methods for their use and production. The disclosed devices may include optically decipherable patterns that facilitate an understanding of binding events between a target molecule and a detection molecule. In one example, a device includes a macroscopic pattern constructed using microscopic elements that can be chromogenically developed to memorialize a binding event. In another example, a device includes characters that can be chromogenically developed using an automated slide staining instrument to memorialize a binding event.

摘要翻译： 本文公开了用于检测靶分子的装置及其使用和生产方法。 所公开的装置可以包括有助于理解目标分子和检测分子之间的结合事件的光学可解译图案。 在一个示例中，设备包括使用可以显色发展以记录绑定事件的微观元素构造的宏观图案。 在另一个示例中，设备包括可以使用自动化幻灯片染色仪器进行染色显影的字符，以记录绑定事件。