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公开(公告)号：US06391541B1
公开(公告)日：2002-05-21
申请号：US09583807
申请日：2000-05-30
申请人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
IPC分类号： G01N3353
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要： A cartridge for separating a desired analyte from a fluid sample has a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for capturing cells or viruses from the sample as the sample flows through the lysing chamber. Beads are also disposed in the lysing chamber for rupturing the cells or viruses to release the analyte therefrom. An analyte flow path extends from the lysing chamber and diverges from the sample flow path. The analyte flow path preferably leads to a reaction chamber for chemically reacting and optically detecting the analyte. The cartridge also includes at least one flow controller (e.g., valves) for directing the sample into the waste chamber after the sample flows through the lysing chamber and for directing the analyte separated from the sample into the analyte flow path.
摘要翻译： 用于从流体样品分离所需分析物的滤筒具有样品流动路径中的样品流动路径和裂解室。 裂解室包含至少一个用于在样品流过裂解室时从样品捕获细胞或病毒的过滤器。 珠也设置在裂解室中，用于破裂细胞或病毒以从其中释放分析物。 分析物流动路径从裂解室延伸并从样品流动路径发散。 分析物流动路径优选地导致用于化学反应和光学检测分析物的反应室。 该盒还包括至少一个流量控制器（例如阀），用于在样品流过裂解室之后将样品引入废物室，并将从样品分离的分析物引导到分析物流动路径中。
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公开(公告)号：US08580559B2
公开(公告)日：2013-11-12
申请号：US11977615
申请日：2007-10-24
申请人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
IPC分类号： C12M1/34
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要： The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译： 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体，裂解细胞或病毒以从其中分离分析物（例如核酸），以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断，生命科学研究，环境或法医应用，用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号：US06881541B2
公开(公告)日：2005-04-19
申请号：US09800590
申请日：2001-03-06
申请人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
IPC分类号： C12M1/33 , B01L3/00 , B01L7/00 , C12M1/26 , C12N1/00 , G01N1/04 , G01N1/10 , G01N33/48 , C12Q1/68
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要： A method for extracting nucleic acid from a fluid sample comprises the steps of introducing the sample into a cartridge having a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for separating cells or viruses from the sample. The sample is forced to flow through the lysing chamber to capture the cells or viruses with the filter, while used sample fluid flows to waste. The captured cells or viruses are disrupted to release their nucleic acid, the nucleic acid is eluted from the lysing chamber, and optionally the nucleic acid is amplified and detected in a reaction chamber of the cartridge.
摘要翻译： 从流体样品中提取核酸的方法包括以下步骤：将样品引入具有样品流动路径中的样品流动路径和裂解室的盒中。 裂解室含有至少一个用于从样品中分离细胞或病毒的过滤器。 样品被迫流过裂解室以用过滤器捕获细胞或病毒，而使用的样品流体流入废物。 捕获的细胞或病毒被破坏以释放其核酸，核酸从裂解室洗脱，并且任选地在核心的反应室中扩增和检测核酸。
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公开(公告)号：US06368871B1
公开(公告)日：2002-04-09
申请号：US08910434
申请日：1997-08-13
申请人： Lee Allan Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi
发明人： Lee Allan Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi
IPC分类号： G01N110
CPC分类号： C07H1/00 , B01F5/0403 , B01F5/0645 , B01F13/0059 , B01F2005/0031 , B01J19/0093 , B01L3/502753 , B01L2200/0636 , B01L2200/0668 , B01L2300/0681 , B01L2300/0816 , B01L2400/0415 , B01L2400/0487 , B01L2400/086 , G01N1/405 , G01N30/6095 , Y10S366/03 , Y10T436/2575
摘要： This invention comprises an apparatus and method for the manipulation of materials, including particles, cells, macromolecules, such as proteins, nucleic acids and other moieties, in fluid samples. The apparatus comprises an enclosed chamber on a chip having an internal microstructure with surface area substantially greater than the facial surface area of the internal structure. Generally the internal microstructure comprises a continuous network of channels, each of which has a depth substantially greater than its width. The network may comprise a single channel, a single channel with multiple branches, multiple channels, multiple channels with multiple branches, and any combination thereof. The internal structure may present an inert, non-reactive surface, or be coated with a reactive ligand, or be electrically conductive and optionally be coated with an electrical insulator. Discrete portions of the internal structure may differ in structural and surface properties. Multiple chips may be linked together to create a multiplexed array of chambers, optionally linked to other analytical devices.
摘要翻译： 本发明包括用于在流体样品中操作包括颗粒，细胞，大分子如蛋白质，核酸和其它部分的材料的装置和方法。 该装置包括在芯片上的封闭室，其具有内表面，其表面面积基本上大于内部结构的面部表面积。 通常，内部微结构包括连续的通道网络，每个通道具有比其宽度大得多的深度。 网络可以包括单个信道，具有多个分支的单个信道，多个信道，具有多个分支的多个信道，以及其任何组合。 内部结构可以呈现惰性的非反应性表面，或者涂覆有反应性配体，或者是导电的，并且任选被电绝缘体涂覆。 内部结构的离散部分在结构和表面性质上可能不同。 可以将多个芯片连接在一起以产生可选地链接到其他分析装置的腔室的多路复用阵列。
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公开(公告)号：US07188001B2
公开(公告)日：2007-03-06
申请号：US10353186
申请日：2003-01-27
申请人： Steven J. Young , Gregory T. A. Kovacs , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Konstantin Othmer , Lee A. Christel
发明人： Steven J. Young , Gregory T. A. Kovacs , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Konstantin Othmer , Lee A. Christel
CPC分类号： B01L7/52 , G05B13/021 , G05D23/1913 , Y10T436/12
摘要： A system for controlling the temperature of a reaction mixture comprises at least one heating device for heating the mixture and a power regulator for regulating the amount of power supplied to the heating device. A controller in communication with the power regulator includes program instructions for heating the reaction mixture by setting a variable target temperature that initially exceeds a desired setpoint temperature for the mixture. When the heating device reaches a threshold temperature, the variable target temperature is decreased to the desired setpoint temperature. In another embodiment, the controller includes an adaptive control program for dynamically adjusting the duration or intensity of power pulses provided to the heating device.
摘要翻译： 用于控制反应混合物的温度的系统包括至少一个用于加热混合物的加热装置和用于调节供应给加热装置的功率量的功率调节器。 与功率调节器通信的控制器包括用于通过设定最初超过混合物的期望设定点温度的可变目标温度来加热反应混合物的程序指令。 当加热装置达到阈值温度时，可变目标温度降低到所需的设定点温度。 在另一个实施例中，控制器包括用于动态调整提供给加热装置的功率脉冲的持续时间或强度的自适应控制程序。
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公开(公告)号：US06979424B2
公开(公告)日：2005-12-27
申请号：US09929270
申请日：2001-08-13
CPC分类号： G01N27/44743 , B01J19/0093 , B01L3/502738 , B01L3/502753 , B01L2300/1883 , B01L2400/0406 , B01L2400/0418 , B01L2400/0421 , B01L2400/0487 , B01L2400/0633 , B01L2400/0655 , G01N30/6095
摘要： An analysis device comprises a body having a reaction chamber for chemically reacting a sample, a separation region for separating components of the sample, and a transition region connecting the reaction chamber to the separation region. The transition region includes at least one valve for controlling the flow of fluid between the reaction chamber and the separation region. Further, the transition region thermally isolates the reaction chamber from the separation region. In a preferred embodiment, the reaction chamber is an amplification chamber for amplifying nucleic acid in the sample, and the separation region comprises an electrophoresis channel containing a suitable matrix material, such as electrophoresis gel or buffer, for separating nucleic acid fragments. Electrodes are embedded in the body for separation of sample components. The body may also be surrounded by external, functional components such as an optical detector for detecting separated components of the sample.
摘要翻译： 分析装置包括具有用于使样品化学反应的反应室的主体，用于分离样品的组分的分离区域和将反应室连接到分离区域的过渡区域。 过渡区域包括用于控制反应室和分离区域之间的流体流动的至少一个阀。 此外，过渡区域将反应室与分离区域热隔离。 在一个优选的实施方案中，反应室是用于扩增样品中的核酸的扩增室，分离区域包含一个电泳通道，其中含有合适的基质材料，如电泳凝胶或缓冲液，用于分离核酸片段。 电极被嵌入在体内用于分离样品组分。 身体也可以被外部功能部件包围，例如用于检测样品的分离成分的光学检测器。
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公开(公告)号：US06818185B1
公开(公告)日：2004-11-16
申请号：US09584328
申请日：2000-05-30
IPC分类号： B01L302
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0858 , B01L2300/087 , B01L2300/123 , B01L2300/14 , B01L2300/168 , B01L2300/18 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12Q1/6844 , G01N33/5302 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： A device for conducting a chemical reaction comprises a body having at least first and second channels formed therein. A reaction vessel extends from the body. The reaction vessel has a reaction chamber, an inlet port connected to the reaction chamber via an inlet channel, and an outlet port connected to the reaction chamber via an outlet channel. The inlet port of the vessel is connected to the first channel in the body, and the outlet port of the vessel is connected to the second channel in the body.
摘要翻译： 用于进行化学反应的装置包括具有形成在其中的至少第一和第二通道的主体。 反应容器从身体延伸出来。 反应容器具有反应室，通过入口通道连接到反应室的入口端口和经由出口通道连接到反应室的出口。 容器的入口连接到体内的第一通道，容器的出口连接到体内的第二通道。
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公开(公告)号：US08168442B2
公开(公告)日：2012-05-01
申请号：US10955811
申请日：2004-09-29
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0858 , B01L2300/087 , B01L2300/123 , B01L2300/14 , B01L2300/168 , B01L2300/18 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12Q1/6844 , G01N33/5302 , Y10T436/25 , Y10T436/25375 , Y10T436/2575
摘要： A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber.
摘要翻译： 用于进行化学反应的药筒包括具有形成在其中的至少一个流路的主体。 药筒还包括从身体延伸的反应容器，用于保持用于化学反应和光学检测的反应混合物。 容器包括限定反应室侧壁的刚性框架。 该框架包括连接到该腔室的流动路径的至少一个通道。 容器还包括附接到刚性框架的相对侧的柔性膜或片，以形成腔室的相对的主壁。 此外，至少两个侧壁是光学透射的并且与每个侧壁成角度地偏移以允许对反应室中的分析物的实时光学检测。
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公开(公告)号：US5958349A
公开(公告)日：1999-09-28
申请号：US808325
申请日：1997-02-28
CPC分类号： B01L3/505 , B01J19/0093 , B01L7/52 , G01N21/0303 , G01N21/0332 , B01J2219/00873
摘要： A reaction vessel for holding a sample for a heat-exchanging chemical process has two opposing major faces and a plurality of contiguous minor faces joining the major faces to each other. The major and minor faces form an enclosed chamber having a triangular-shaped bottom portion. The ratio of the thermal conductance of the major faces to that of the minor faces is at least 2:1, and the minor faces forming the triangular-shaped bottom portion of the chamber are optically transmissive. The vessel also has a port for introducing a sample into the chamber and a cap for sealing the chamber.
摘要翻译： 用于保持用于热交换化学过程的样品的反应容器具有两个相对的主面和将主面彼此连接的多个连续的小面。 主面和次面形成具有三角形底部的封闭室。 主面与小面的热导率之比至少为2:1，形成腔的三角形底部的小面光学透光。 容器还具有用于将样品引入室中的端口和用于密封室的盖。
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公开(公告)号：US20080286151A1
公开(公告)日：2008-11-20
申请号：US10719073
申请日：2003-11-21
IPC分类号： G01N33/48
CPC分类号： B01L7/52 , B01J19/0093 , B01J2219/00788 , B01J2219/00873 , B01J2219/00963 , B01J2219/0097 , B01L3/508 , B01L9/00 , B01L2300/042 , B01L2300/0858 , B01L2300/1827 , F28D2021/0077 , F28F13/00 , G01N21/0332 , G01N35/0099 , G01N2035/00376 , G01N2035/0405 , Y10T436/2575
摘要： An apparatus for controlling the temperature of a reaction mixture contained in a chamber of a reaction vessel comprises a thermal surface for contacting a flexible wall of the chamber and an automated machine for increasing the pressure in the chamber. The pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface for good thermal conductance. The apparatus also includes at least one thermal element for heating or cooling the surface to induce a temperature change within the chamber.
摘要翻译： 用于控制包含在反应容器的室中的反应混合物的温度的装置包括用于接触室的柔性壁的热表面和用于增加室中的压力的自动化机器。 室中的压力增加足以迫使柔性壁与热表面一致，以获得良好的导热性。 该装置还包括至少一个热元件，用于加热或冷却表面以引起腔室内的温度变化。
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