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公开(公告)号：US06565815B1
公开(公告)日：2003-05-20
申请号：US09194374
申请日：2000-07-25
申请人： Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人： Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
IPC分类号： F28D700
CPC分类号： G01N21/75 , B01J19/0093 , B01J2219/00783 , B01J2219/0081 , B01J2219/00822 , B01J2219/00828 , B01J2219/00833 , B01J2219/00871 , B01J2219/00873 , B01J2219/00961 , B01J2219/0097 , B01J2219/00986 , B01L3/505 , B01L3/508 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要： The present invention provides a reaction vessel and apparatus for performing heat-exchanging reactions. The vessel has a chamber for holding a sample, the chamber being defined by a plurality of walls, at least two of the walls being light transmissive to provide optical windows to the chamber. The apparatus comprises at least one heating surface for contacting at least one of the plurality of walls, a heat source for heating the surface, and optics positioned to optically interrogate the chamber while the heating surface is in contact with at least one of the plurality of walls. The optics include at least one light source for transmitting light to the chamber through a first one of the light transmissive walls and at least one detector for detecting light exiting the chamber through a second one of the light transmissive walls.
摘要翻译： 本发明提供一种用于进行热交换反应的反应容器和装置。 容器具有用于保持样品的室，所述室由多个壁限定，至少两个壁是透光的，以向室提供光学窗口。 该装置包括用于接触多个壁中的至少一个的至少一个加热表面，用于加热该表面的热源，以及定位成光学询问该室的光学器件，同时该加热表面与多个壁板中的至少一个接触 墙壁 所述光学器件包括至少一个光源，用于通过第一透光壁将光传输到室，以及至少一个检测器，用于检测通过第二透光壁离开室的光。
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公开(公告)号：US08029733B2
公开(公告)日：2011-10-04
申请号：US11981155
申请日：2007-10-30
申请人： Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人： Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
CPC分类号： G01N21/75 , B01J19/0093 , B01J2219/00783 , B01J2219/0081 , B01J2219/00822 , B01J2219/00828 , B01J2219/00833 , B01J2219/00871 , B01J2219/00873 , B01J2219/00961 , B01J2219/0097 , B01J2219/00986 , B01L3/505 , B01L3/508 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要： A reaction vessel having a reaction chamber for holding a sample is fabricated by producing a housing having a rigid frame defining the minor walls of the chamber. The housing also defines a port for introducing fluid into the chamber. At least one sheet or film is attached to the rigid frame to form at least one major wall of the chamber. In preferred embodiments, two sheets or films are attached to opposite sides of the rigid frame to form two opposing major walls of the chamber, the major walls being connected to each other by the minor walls.
摘要翻译： 具有用于保持样品的反应室的反应容器通过制造具有限定室的小壁的刚性框架的壳体来制造。 壳体还限定了用于将流体引入腔室的端口。 至少一个片或薄膜连接到刚性框架以形成腔室的至少一个主壁。 在优选实施例中，两个片或膜连接到刚性框架的相对侧面以形成腔室的两个相对的主壁，主壁通过次壁彼此连接。
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公开(公告)号：US07188001B2
公开(公告)日：2007-03-06
申请号：US10353186
申请日：2003-01-27
申请人： Steven J. Young , Gregory T. A. Kovacs , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Konstantin Othmer , Lee A. Christel
发明人： Steven J. Young , Gregory T. A. Kovacs , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Konstantin Othmer , Lee A. Christel
CPC分类号： B01L7/52 , G05B13/021 , G05D23/1913 , Y10T436/12
摘要： A system for controlling the temperature of a reaction mixture comprises at least one heating device for heating the mixture and a power regulator for regulating the amount of power supplied to the heating device. A controller in communication with the power regulator includes program instructions for heating the reaction mixture by setting a variable target temperature that initially exceeds a desired setpoint temperature for the mixture. When the heating device reaches a threshold temperature, the variable target temperature is decreased to the desired setpoint temperature. In another embodiment, the controller includes an adaptive control program for dynamically adjusting the duration or intensity of power pulses provided to the heating device.
摘要翻译： 用于控制反应混合物的温度的系统包括至少一个用于加热混合物的加热装置和用于调节供应给加热装置的功率量的功率调节器。 与功率调节器通信的控制器包括用于通过设定最初超过混合物的期望设定点温度的可变目标温度来加热反应混合物的程序指令。 当加热装置达到阈值温度时，可变目标温度降低到所需的设定点温度。 在另一个实施例中，控制器包括用于动态调整提供给加热装置的功率脉冲的持续时间或强度的自适应控制程序。
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4.发明授权公开(公告)号：US07255833B2
公开(公告)日：2007-08-14
申请号：US10379291
申请日：2003-03-03
申请人： Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人： Ronald Chang , Lee A. Christel , Gregory T. A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
IPC分类号： G01N21/00
CPC分类号： B01L3/508 , B01J19/0093 , B01J2219/00804 , B01J2219/0081 , B01J2219/00873 , B01J2219/0097 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要： This invention provides an apparatus for rapidly heating and/or cooling a sample in a reaction vessel. In some embodiments, the apparatus includes optics for the efficient detection of a reaction product in the vessel. The invention also provides a reaction vessel having a reaction chamber designed for optimal thermal conductance and for efficient optical viewing of reaction products in the chamber.
摘要翻译： 本发明提供一种用于在反应容器中快速加热和/或冷却样品的装置。 在一些实施例中，该装置包括用于有效检测容器中的反应产物的光学元件。 本发明还提供一种反应容器，该反应容器具有设计用于最佳热传导和有效地光学观察室内反应产物的反应室。
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公开(公告)号：US06940598B2
公开(公告)日：2005-09-06
申请号：US10119228
申请日：2002-04-08
申请人： Lee A. Christel , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs , Steven J. Young , Ronald Chang , Douglas B. Dority , Raymond T. Hebert , Gregory J. Kintz
发明人： Lee A. Christel , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs , Steven J. Young , Ronald Chang , Douglas B. Dority , Raymond T. Hebert , Gregory J. Kintz
IPC分类号： B01J19/00 , B01L3/00 , B01L3/14 , B01L7/00 , F28F3/12 , F28F21/04 , G01N21/03 , G01N35/00 , G01N21/64
CPC分类号： B01L3/508 , B01J19/0093 , B01L3/5082 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要： An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel [2] having a chamber [10] for holding the mixture. The apparatus also includes a heat-exchanging module [37] having a pair of opposing thermal plates [34A, 34B] for receiving the vessel [2] between them and for heating/and or cooling the mixture contained in the vessel. The module [37] also includes optical excitation and detection assemblies [46,48] positioned to optically interrogate the mixture. The excitation assembly [46] includes multiple light sources [100] and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly [48] includes multiple detectors [102] and a second set of filters for detecting light emitted from the chamber [10] in multiple emission wavelength ranges. The optics assemblies [46,48] thus provide a multi-channel system for detecting a plurality of different target analytes in the mixture.
摘要翻译： 用于热控制和光学询问反应混合物的装置包括具有用于保持混合物的室[10]的容器[2]。 该装置还包括具有一对相对的热板[34A，34B]的热交换模块[37]，用于在它们之间接收容器[2]并用于加热和/或冷却容器中所含的混合物。 模块[37]还包括定位成光学询问混合物的光学激发和检测组件[46,48]。 激发组件[46]包括多个光源[100]和一组用于在多个激发波长范围内用激发光束顺序地照射混合物中的标记分析物的滤光器。 检测组件[48]包括多个检测器[102]和第二组滤波器，用于在多个发射波长范围内检测从腔室[10]发射的光。 因此，光学组件[46,48]提供用于检测混合物中的多个不同目标分析物的多通道系统。
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公开(公告)号：US5958349A
公开(公告)日：1999-09-28
申请号：US808325
申请日：1997-02-28
CPC分类号： B01L3/505 , B01J19/0093 , B01L7/52 , G01N21/0303 , G01N21/0332 , B01J2219/00873
摘要： A reaction vessel for holding a sample for a heat-exchanging chemical process has two opposing major faces and a plurality of contiguous minor faces joining the major faces to each other. The major and minor faces form an enclosed chamber having a triangular-shaped bottom portion. The ratio of the thermal conductance of the major faces to that of the minor faces is at least 2:1, and the minor faces forming the triangular-shaped bottom portion of the chamber are optically transmissive. The vessel also has a port for introducing a sample into the chamber and a cap for sealing the chamber.
摘要翻译： 用于保持用于热交换化学过程的样品的反应容器具有两个相对的主面和将主面彼此连接的多个连续的小面。 主面和次面形成具有三角形底部的封闭室。 主面与小面的热导率之比至少为2:1，形成腔的三角形底部的小面光学透光。 容器还具有用于将样品引入室中的端口和用于密封室的盖。
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公开(公告)号：US06369893B1
公开(公告)日：2002-04-09
申请号：US09314605
申请日：1999-05-19
申请人： Lee A. Christel , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs , Steven J. Young , Ronald Chang , Douglas B. Dority , Raymond T. Hebert , Gregory J. Kintz
发明人： Lee A. Christel , M. Allen Northrup , Kurt E. Petersen , William A. McMillan , Gregory T. A. Kovacs , Steven J. Young , Ronald Chang , Douglas B. Dority , Raymond T. Hebert , Gregory J. Kintz
IPC分类号： G01N2164
CPC分类号： B01L3/508 , B01J19/0093 , B01L3/5082 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要： An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel [2] having a chamber [10] for holding the mixture. The apparatus also includes a heat-exchanging module [37] having a pair of opposing thermal plates [34A, 34B] for receiving the vessel [2] between them and for heating/and or cooling the mixture contained in the vessel. The module [37] also includes optical excitation and detection assemblies [46,48] positioned to optically interrogate the mixture. The excitation assembly [46] includes multiple light sources [100] and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly [48] includes multiple detectors [102] and a second set of filters for detecting light emitted from the chamber [10] in multiple emission wavelength ranges. The optics assemblies [46,48] thus provide a multi-channel system for detecting a plurality of different target analytes in the mixture.
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公开(公告)号：US20080254532A1
公开(公告)日：2008-10-16
申请号：US11981155
申请日：2007-10-30
申请人： Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
发明人： Ronald Chang , Lee A. Christel , Gregory T.A. Kovacs , William A. McMillan , M. Allen Northrup , Kurt E. Petersen , Farzad Pourahmadi , Steven J. Young , Robert Yuan , Douglas B. Dority
IPC分类号： C12M1/02
CPC分类号： G01N21/75 , B01J19/0093 , B01J2219/00783 , B01J2219/0081 , B01J2219/00822 , B01J2219/00828 , B01J2219/00833 , B01J2219/00871 , B01J2219/00873 , B01J2219/00961 , B01J2219/0097 , B01J2219/00986 , B01L3/505 , B01L3/508 , B01L7/52 , B01L2300/042 , B01L2300/0809 , B01L2300/0858 , B01L2300/168 , B01L2300/1805 , F28D2021/0077 , F28F3/12 , F28F21/04 , F28F2280/10 , G01N21/0303 , G01N21/0332 , G01N2035/00326
摘要： A reaction vessel having a reaction chamber for holding a sample is fabricated by producing a housing having a rigid frame defining the minor walls of the chamber. The housing also defines a port for introducing fluid into the chamber. At least one sheet or film is attached to the rigid frame to form at least one major wall of the chamber. In preferred embodiments, two sheets or films are attached to opposite sides of the rigid frame to form two opposing major walls of the chamber, the major walls being connected to each other by the minor walls.
摘要翻译： 具有用于保持样品的反应室的反应容器通过制造具有限定室的小壁的刚性框架的壳体来制造。 壳体还限定了用于将流体引入腔室的端口。 至少一个片或薄膜连接到刚性框架以形成腔室的至少一个主壁。 在优选实施例中，两个片或膜连接到刚性框架的相对侧面以形成腔室的两个相对的主壁，主壁通过次壁彼此连接。
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公开(公告)号：US06942971B2
公开(公告)日：2005-09-13
申请号：US10027404
申请日：2001-12-19
IPC分类号： B01L7/00 , C07H21/02 , C07H21/04 , C12M1/00 , C12M1/34 , C12N9/98 , C12P19/34 , C12Q1/00 , C12Q1/68 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/50 , G01N35/00 , G06F19/00
CPC分类号： C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要： An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.
摘要翻译： 用于确定核酸扩增反应中的阈值（例如，阈值循环数或时间值）的装置包括检测机制，用于在扩增反应期间的多个不同时间测量至少一个强度为 与在反应中被扩增的核酸序列的量有关。 与检测机制通信的控制器被编程为存储定义核酸序列的生长曲线的信号值，确定生长曲线的导数，并计算与该衍生物的特征相关联的周期数或时间值。
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公开(公告)号：US06911327B2
公开(公告)日：2005-06-28
申请号：US09808706
申请日：2001-03-14
IPC分类号： B01L7/00 , C07H21/02 , C07H21/04 , C12M1/00 , C12M1/34 , C12N9/98 , C12P19/34 , C12Q1/00 , C12Q1/68 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/50 , G01N35/00 , G06F19/00
CPC分类号： C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要： A method for determining an unknown starting quantity of a target nucleic acid sequence in a test sample comprises amplifying the unknown starting quantity of the target nucleic acid sequence in the test sample and known starting quantities of a calibration nucleic acid sequence in respective calibration samples; and determining a respective threshold value for each of the nucleic acid sequences using a derivative of a growth curve derived for the sequence. The starting quantity of the target nucleic acid sequence in the test sample is determined using the threshold value determined for the target sequence and a calibration curve derived from the threshold values determined for the known starting quantities of the calibration nucleic acid sequences.
摘要翻译： 用于测定测试样品中目标核酸序列的未知起始量的方法包括扩增测试样品中目标核酸序列的未知起始量和已知的各校准样品中校准核酸序列的起始量; 以及使用由该序列导出的生长曲线的衍生物来确定每个核酸序列的相应阈值。 使用针对靶序列确定的阈值和从针对校准核酸序列的已知起始量确定的阈值得到的校准曲线来确定测试样品中靶核酸序列的起始量。
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