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公开(公告)号：US07582431B2
公开(公告)日：2009-09-01
申请号：US10987549
申请日：2004-11-12
申请人： Radoje Drmanac , Snezana Drmanac , David Kita , Cory Cooke , Chongjun Xu
发明人： Radoje Drmanac , Snezana Drmanac , David Kita , Cory Cooke , Chongjun Xu
CPC分类号： C12Q1/6874 , C12Q2537/165
摘要： The invention provides methods for sequencing by hybridization (SBH) using pools of probes that allow greater efficiency in conducting SBH by reducing the number of separate measurements of hybridization signals required to identify each particular nucleotide in a target nucleic acid sequence. The invention also provides pools and sets of pools of probes, as well as methods of generating pools of probes.
摘要翻译： 本发明提供了通过杂交（SBH）进行测序的方法，所述探针池通过减少鉴定靶核酸序列中的每个特定核苷酸所需的杂交信号的单独测量的数量，从而能够更高效地传导SBH。 本发明还提供了一组探针池和一组探测池的生成方法。
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公开(公告)号：US20110039731A1
公开(公告)日：2011-02-17
申请号：US12644860
申请日：2009-12-22
申请人： Radoje Drmanac , Snezana Drmanac , David Kita , Cory Cooke , Chongjun Xu
发明人： Radoje Drmanac , Snezana Drmanac , David Kita , Cory Cooke , Chongjun Xu
IPC分类号： C40B40/06
CPC分类号： C12Q1/6874 , C12Q2537/165
摘要： The invention provides methods for sequencing by hybridization (SBH) using pools of probes that allow greater efficiency in conducting SBH by reducing the number of separate measurements of hybridization signals required to identify each particular nucleotide in a target nucleic acid sequence. The invention also provides pools and set of pools of probes, as well as method of generating pools of probes.
摘要翻译： 本发明提供了通过杂交（SBH）进行测序的方法，所述探针池通过减少鉴定靶核酸序列中的每个特定核苷酸所需的杂交信号的单独测量的数量，从而能够更高效地传导SBH。 本发明还提供了池和一组探测池，以及生成探针池的方法。
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公开(公告)号：US20050191656A1
公开(公告)日：2005-09-01
申请号：US10987549
申请日：2004-11-12
申请人： Radoje Drmanac , Snezana Drmanac , David Kita , Cory Cooke , Chongjun Xu
发明人： Radoje Drmanac , Snezana Drmanac , David Kita , Cory Cooke , Chongjun Xu
CPC分类号： C12Q1/6874 , C12Q2537/165
摘要： The invention provides methods for sequencing by hybridization (SBH) using pools of probes that allow greater efficiency in conducting SBH by reducing the number of separate measurements of hybridization signals required to identify each particular nucleotide in a target nucleic acid sequence. The invention also provides pools and sets of pools of probes, as well as methods of generating pools of probes.
摘要翻译： 本发明提供了通过杂交（SBH）进行测序的方法，所述探针池通过减少鉴定靶核酸序列中的每个特定核苷酸所需的杂交信号的单独测量的数量，从而能够更高效地传导SBH。 本发明还提供了一组探针池和一组探测池的生成方法。
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公开(公告)号：US20070060743A1
公开(公告)日：2007-03-15
申请号：US10276817
申请日：2001-05-16
申请人： Y. Tom Tang , Chenghua Liu , Radoje Drmanac
发明人： Y. Tom Tang , Chenghua Liu , Radoje Drmanac
CPC分类号： C07K14/47
摘要： The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof.
摘要翻译： 本发明提供了新的核酸，由这些核酸编码的新的多肽序列及其用途。
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25.发明申请Method and materials relating to novel stem cell growth factor-like polypeptides and polynucleotides 失效与新型干细胞生长因子样多肽和多核苷酸相关的方法和材料公开(公告)号：US20050059073A1
公开(公告)日：2005-03-17
申请号：US10968674
申请日：2004-10-19
申请人： Y. Tang , Ivan Labat , Radoje Drmanac , Nancy Mize , Mitsuo Nishikawa , Cheng-Chi Chao
发明人： Y. Tang , Ivan Labat , Radoje Drmanac , Nancy Mize , Mitsuo Nishikawa , Cheng-Chi Chao
IPC分类号： A61K38/00 , A61K48/00 , C07K14/475 , C07K16/00 , C12N5/08 , C12N9/16 , C12N9/64 , C12Q1/68 , C07H21/04
CPC分类号： C07K14/475 , A01K2217/05 , A61K38/00 , A61K48/00 , C07K16/00 , C07K2319/00 , C12N9/16 , C12N9/6432 , C12Y304/21006 , Y10S435/81 , Y10S435/975
摘要： The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human stem cell growth factor-like protein. These polynucleotides comprise nucleic acid sequences isolated from cDNA libraries from human testis cells (Hyseq clone identification numbers 2880984 and 2881695), from human fetal skin (Hyseq clone identification number 15375176), adult spleen (Hyseq clone identification number 14856094), and human endothelial cells (Hyseq clone identification numbers 13804756, 13687487, 13804756). Other aspects of the invention include vectors containing processes for producing novel human stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.
摘要翻译： 本发明提供由对应于新的人类干细胞生长因子样蛋白质的这种多核苷酸及其突变体或变体编码的新型多核苷酸和多肽。 这些多核苷酸包含从人胎儿皮肤（Hyseq克隆鉴定号15375176），成年脾（Hyseq克隆鉴定号14856094）和人内皮细胞的人睾丸细胞（Hyseq克隆鉴定号2880984和2881695）的cDNA文库中分离的核酸序列 （Hyseq克隆鉴定号13804756,13687487,13804756）。 本发明的其它方面包括含有生产新型人类干细胞生长因子样多肽的方法的载体和对这些多肽特异的抗体。
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公开(公告)号：US09551026B2
公开(公告)日：2017-01-24
申请号：US13337968
申请日：2011-12-27
申请人： Andres Fernandez , Bryan Staker , Radoje Drmanac
发明人： Andres Fernandez , Bryan Staker , Radoje Drmanac
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6825 , C12Q2523/307 , C12Q2565/501 , C12Q2565/607
摘要： Methods are provided for carrying out DNA sequencing on a device having upper and lower conductive layers separated by an insulative layer. Holes in the upper conductive layer create discrete attachment sites for DNA fragments. Voltage is applied to the surface to control affinity between the attachment sites and the DNA fragments, and to compact the DNA fragments for discrete optical detection.
摘要翻译： 提供了用于在具有由绝缘层隔开的上导电层和下导电层的器件上进行DNA测序的方法。 上导电层中的孔为DNA片段产生离散的附着位点。 将电压施加到表面以控制附着位点和DNA片段之间的亲和力，并压缩DNA片段以进行离散的光学检测。
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公开(公告)号：US09023769B2
公开(公告)日：2015-05-05
申请号：US12956802
申请日：2010-11-30
申请人： Radoje Drmanac , Fredrik Dahl , Evan Hurowitz , Fredrie Dahl
发明人： Radoje Drmanac , Fredrik Dahl , Evan Hurowitz , Fredrie Dahl
CPC分类号： C12Q1/6869 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12N15/10 , C12Q1/6853 , C40B20/00
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译： 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。
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公开(公告)号：US08617811B2
公开(公告)日：2013-12-31
申请号：US12361507
申请日：2009-01-28
申请人： Radoje Drmanac
发明人： Radoje Drmanac
CPC分类号： C12Q1/6874 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6876 , C12Q2565/102 , C12Q2565/1025 , C12Q2565/518
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences. In particular, the present invention provides methods and compositions for improving the efficiency of sequencing reactions by using fewer labels to distinguish between nucleotides and by detecting nucleotides at multiple detection positions in a target sequence.
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公开(公告)号：US08592150B2
公开(公告)日：2013-11-26
申请号：US12816365
申请日：2010-06-15
申请人： Radoje Drmanac , Brock A. Peters , Andrei Alexeev , Peter Hong
发明人： Radoje Drmanac , Brock A. Peters , Andrei Alexeev , Peter Hong
CPC分类号： B01J19/0046 , B01J2219/00722 , C12P19/34 , C12Q1/6869 , C12Q1/6874 , C12Q2525/101 , C12Q2521/101
摘要： The present invention is directed to methods and compositions for long fragment read sequencing. The present invention encompasses methods and compositions for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data.
摘要翻译： 本发明涉及用于长片段读取测序的方法和组合物。 本发明包括用于制备基因组DNA长片段的方法和组合物，用于处理长片段读取测序方法的基因组DNA，以及用于处理和分析序列数据的软件和算法。
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公开(公告)号：US08440397B2
公开(公告)日：2013-05-14
申请号：US11981761
申请日：2007-10-31
CPC分类号： C12Q1/6874 , C12N15/64 , C12N15/66 , Y10T436/143333 , C12Q2521/313 , C12Q2525/191 , C12Q2525/151 , C12Q2525/131 , C12Q2565/518 , C12Q2533/107 , C12Q2531/125
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.
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