公开(公告)号：US5385830A

公开(公告)日：1995-01-31

申请号：US771632

申请日：1991-10-04

申请人： Yoshifumi Amano ,  Kazuo Nakamura ,  Hiroshi Kurosawa ,  Takeshi Sato ,  Hirofumi Akano ,  Yoshiya Kawamura

发明人： Yoshifumi Amano ,  Kazuo Nakamura ,  Hiroshi Kurosawa ,  Takeshi Sato ,  Hirofumi Akano ,  Yoshiya Kawamura

IPC分类号： C12Q1/00 ,  C12Q1/02 ,  C12Q1/26 ,  C12Q1/32 ,  C12S3/00 ,  C12M1/00 ,  C12P1/04 ,  C12P11/00

CPC分类号： C12Q1/02 ,  C12Q1/001 ,  C12Q1/26 ,  C12Q1/32 ,  Y10T436/15 ,  Y10T436/18 ,  Y10T436/184 ,  Y10T436/186 ,  Y10T436/188

摘要： The present invention provides a method for measuring the amount of free sulfurous acid and bound sulfurous acid in a sample using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans of in which the sample is treated with acid and/or alkali to give free sulfurous acid alone in the sample. Then, the total amount of free sulfurous acid in the sample is measured by an oxidation reaction of free sulfurous acid to sulfuric acid using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans.

摘要翻译： 本发明提供了使用属于硫氧化硫杆菌（Thiobacillus thiooxidans）或硫氧化硫杆菌（Thiobacillus ferrooxidans）的细菌测量样品中游离亚硫酸和结合亚硫酸的量的方法，其中样品用酸和/或碱处理以产生单独的亚硫酸 在样品中。 然后，使用属于硫氧化硫杆菌（Thiobacillus thiooxidans）或硫氧化硫杆菌（Thiobacillus ferrooxidans）的细菌，通过游离亚硫酸与硫酸的氧化反应测定样品中游离亚硫酸的总量。

公开(公告)号：US5356792A

公开(公告)日：1994-10-18

申请号：US978843

申请日：1992-11-19

申请人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Hatagaki Keizo ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

发明人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Hatagaki Keizo ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

IPC分类号： C12Q1/00 ,  G01N33/18 ,  C12Q1/24 ,  G01N27/40

CPC分类号： G01N33/1806 ,  C12Q1/001 ,  G01N33/1866 ,  G01N33/1893 ,  Y10S435/817 ,  Y10S435/852

摘要： The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in membrane. Specifically, the present invention relates to a BOD analyzer comprising flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from the group consisting of alginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.

摘要翻译： 本发明涉及一种包含含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，本发明涉及一种BOD分析装置，其特征在于，包括配置有含有氧电极和微生物膜的微生物传感器的流动池， 以及与流通池的入口连接且配有出口的液体通道。 通过使用至少一种选自藻酸或其盐的凝胶剂，将属于克雷伯氏菌12092菌株的微生物固定在直径为0.65-3μm平均孔径的多孔亲水膜中来制备微生物膜 ，琼脂，结冷胶，黄原胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶和支链淀粉。 本发明还涉及使用BOD分析仪分析BOD的改进方法。

公开(公告)号：US4996064A

公开(公告)日：1991-02-26

申请号：US396662

申请日：1989-08-22

申请人： Hirofumi Akano ,  Takeshi Sato ,  Hajime Okumura ,  Yoshiya Kawamura ,  Kyo Shimada

发明人： Hirofumi Akano ,  Takeshi Sato ,  Hajime Okumura ,  Yoshiya Kawamura ,  Kyo Shimada

IPC分类号： A23C20/02 ,  A23L11/00

CPC分类号： A23C20/025 ,  A23L11/09

摘要： A novel foodstuff is produced by reacting a milk-coagulating enzyme which is produced by a milk-coagulating enzyme-producing microorganism belonging to genus Aspergillus, genus Mucor, or genus Rhizopus and which exhibits a milk-coagulating activity (A) and a protease activity (B) such that the ratio of (A)/(B) is larger than 0.1, with soymilk thereby inducing coagulation, and collecting a resulting coagulated material. The novel foodstuffs produced have the benefit of lacking the bitterness and astringency common to soybean, abounds in emulsifying activity, exhibit a high water-retention property, and are smoothly agreeable to the taste. Thus, they have extensive utility as substitutes for raw materials in conventional processed foods. They are especially suitable for use as raw materials for emulsifiable foods.

摘要翻译： 通过使属于曲霉属，毛霉属或根霉属的乳凝固酶生成微生物产生的乳化凝固酶与乳固醇活性（A）和蛋白酶活性（A）反应而制造的新型食品 （B）使得（A）/（B）的比例大于0.1，用豆浆从而引起凝结，并收集所得的凝固材料。 所生产的新型食品具有缺乏大豆常见的苦味和涩味的优点，乳化活性大，显示出高保水性，并且味道顺利。 因此，它们作为常规加工食品中原料的替代物具有广泛的用途。 它们特别适合用作可乳化食品的原料。

公开(公告)号：US4692414A

公开(公告)日：1987-09-08

申请号：US938166

申请日：1986-12-04

申请人： Mikio Yamada ,  Yoshinori Tsukamoto ,  Masahiro Mizuno

发明人： Mikio Yamada ,  Yoshinori Tsukamoto ,  Masahiro Mizuno

IPC分类号： C12J1/04 ,  C12J1/10 ,  C12M1/36 ,  C12M1/34

CPC分类号： C12M41/32 ,  C12J1/10 ,  C12M41/48 ,  Y10S435/818

摘要： A method and apparatus for producing vinegar by acetic acid fermentation utilizing an automatic alcohol analyzer for measurement of alcohol concentration in a fermenting broth, a feeding apparatus for feeding alcohol solution as raw material to the fermenting broth, and a control computer controllably coupled to the alcohol analyzer and to the feeding apparatus. The alcohol concentration in the fermenting broth is periodically measured by the automatic alcohol analyzer by a direction from the control computer at given intervals over a plurality of cycles of measurement; the control computer calculates a feed rate of alcohol solution as raw material to the fermenting broth so as to control the alcohol concentration in the fermenting broth at a desirable concentration on the basis of a difference between a measured alcohol concentration in the fermenting broth and a desirable alcohol concentration and the alcohol consumption rate calculated during each of the cycles by the control computer; and then the feeding apparatus is operated to control the feed rate of the alcohol solution responsive to a control signal generated by the control computer as a result of the aforementioned calculations, to thereby controllably feed alcohol solution as raw material to the fermenting broth.

摘要翻译： 一种通过乙酸发酵生产醋的方法和装置，使用用于测量发酵液中酒精浓度的自动酒精分析仪，用于将醇溶液作为原料供给到发酵液中的进料装置，以及可控地与醇连接的对照计算机 分析仪和送料装置。 在多个测量循环中，通过自动酒精分析仪通过来自控制计算机的方向以给定间隔周期性地测量发酵液中的酒精浓度; 控制计算机计算作为原料的醇溶液的进料速率，以发酵液为基础，以发酵液中测定的酒精浓度与所需发酵液之间的差为基准，将发酵液中的醇浓度控制在理想浓度 酒精浓度和通过控制计算机在每个循环期间计算的酒精消耗率; 然后根据由上述计算结果控制由控制计算机产生的控制信号来控制进料装置，从而可控制地将醇溶液作为原料供给发酵液。

公开(公告)号：US4656140A

公开(公告)日：1987-04-07

申请号：US669761

申请日：1984-11-08

申请人： Mikio Yamada ,  Masahiro Mizuno ,  Yoshinori Tsukamoto ,  Koki Yamada

发明人： Mikio Yamada ,  Masahiro Mizuno ,  Yoshinori Tsukamoto ,  Koki Yamada

IPC分类号： C12J1/04 ,  C12J1/10 ,  C12Q1/00 ,  G01N1/22 ,  G01N30/00 ,  G01N33/00 ,  G01N33/14 ,  B01D15/08 ,  C12P7/54 ,  G01N1/00

CPC分类号： G01N33/146 ,  G01N1/2247 ,  G01N30/00 ,  G01N33/0014 ,  Y10S435/807 ,  Y10T436/203332 ,  Y10T436/204165 ,  Y10T436/25125 ,  Y10T436/255 ,  Y10T436/25875

摘要： A method for measuring the alcohol concentration of an acetic acid fermentation broth which comprises (a) forming a sample gas containing volatile components present in the acetic acid fermentation broth, the volatile componentscomprising alcohol and acetic acid; (b) passing the sample gas through an absorption column packed with a material which binds acetic acid whereby the acetic acid is absorbed on the material; (c) passing the sample gas which has passed through the absorption column to a gas sensor for measuring the alcohol content of the gas by outputting an electrical signal which correlates to the alcohol content of the sample gas; and (d) determining the alcohol content of the sample gas in response to the electrical output signal from the gas sensor. The invention also provides a method of forming the sample gas by passing a sample of the fermentation broth through a water-repellent gas permeable tube mounted in a hollow cell and passing a carrier gas through the hollow cell whereby volatile components from the fermentation broth inside of the tube diffuse through the gas permeable tube wall and are intermixed with the carrier gas to form the sample gas containing the volatile components.

摘要翻译： 一种乙酸发酵液的酒精浓度的测定方法，其特征在于，包括：（a）形成含有存在于乙酸发酵液中的挥发性成分的样品气体，含有挥发性成分的醇和乙酸; （b）使样品气体通过填充有结合乙酸的材料的吸收塔，由此乙酸被吸收在材料上; （c）将通过吸收塔的样品气体通过输出与样品气体的醇含量相关的电信号，将气体传感器通过气体传感器测量气体的醇含量; 和（d）响应于来自气体传感器的电输出信号确定样品气体的醇含量。 本发明还提供了一种通过将发酵液的样品通过安装在中空细胞中的防水透气管并使载气通过中空细胞而形成样品气体的方法，由此来自发酵液中的挥发性组分 管扩散通过透气管壁并与载气混合形成含有挥发性成分的样品气体。

公开(公告)号：US4282257A

公开(公告)日：1981-08-04

申请号：US115419

申请日：1980-01-25

申请人： Yoshio Kunimatsu ,  Hajime Okumura ,  Hiroshi Masai ,  Koki Yamada ,  Mikio Yamada

发明人： Yoshio Kunimatsu ,  Hajime Okumura ,  Hiroshi Masai ,  Koki Yamada ,  Mikio Yamada

IPC分类号： C12J1/04 ,  C12J1/00 ,  C12P7/54

CPC分类号： C12J1/04

摘要： Vinegar having an acetic acid concentration higher than 18 percent weight by volume is produced in a submerged fermentation by maintaining the temperature of the fermenting broth after the initiation of the fermentation at 27.degree.-32.degree. C. until the acetic concentration of the fermenting broth reaches 12-15 percent weight by volume and thereafter maintaining the temperature of the fermenting broth at 18.degree.-24.degree. C.

摘要翻译： 醋酸浓度高于18％（重量）的醋通过将发酵开始后的发酵液的温度保持在27℃-32℃直到发酵液的乙酸浓度达到 12-15重量％，然后将发酵液的温度保持在18〜24℃。