Tissue grafts and methods of making and using the same

    公开(公告)号:US11471285B2

    公开(公告)日:2022-10-18

    申请号:US15108996

    申请日:2014-12-29

    摘要: In some embodiments, the present invention provides tissue grafts, such as vascularized bone grafts, and methods for preparing and using such tissue grafts. In some embodiments the tissue grafts are made using pluripotent stem cells, such as autologous pluripotent stem cells. In some embodiments, the tissue grafts are made by creating a digital model of a tissue portion to be replaced or repaired, such as a bone defect, partitioning the model into two or more model segments, and then producing tissue graft segments having a size and shape corresponding to that of the model segments. Such tissue graft segments may be assembled to form a tissue graft having a size and shape corresponding to that of the tissue portion to be replaced or repaired.

    Perfusion bioreactor
    25.
    发明授权

    公开(公告)号:US10214714B2

    公开(公告)日:2019-02-26

    申请号:US14959950

    申请日:2015-12-04

    IPC分类号: C12M1/00 C12M1/12 C12M3/00

    摘要: In some embodiments the present invention provides perfusion bioreactors and cell culture scaffolds suitable for the preparation of tissue grafts, such as bone tissue grafts. In some embodiments, the perfusion bioreactors comprise a graft chamber and/or a graft chamber insert configured to hold a tissue graft having a certain shape and/or certain dimensions, and/or to allow culture of such tissue grafts under press-fit direct perfusion conditions. In some embodiments, the perfusion bioreactors comprise an equilibration chamber.