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79 条记录 (耗时 0.013 秒)

    Process for the microbial production of alcoholoxidase
    21.
    发明授权
    Process for the microbial production of alcoholoxidase 失效
    藻酸酶微生物生产工艺

    公开(公告)号：US4250261A

    公开(公告)日：1981-02-10

    申请号：US55533

    申请日：1979-07-09

    申请人： Lothar Eggeling ,  Manfred Paschke ,  Hermann Sahm

    发明人： Lothar Eggeling ,  Manfred Paschke ,  Hermann Sahm

    IPC分类号： C12N9/04 ,  C12R1/78

    CPC分类号： C12N9/0006 ,  Y10S435/93

    摘要： A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.

    摘要翻译： 一种生产醇氧化酶的方法，其中将多形汉逊酵母（Hansenula polymorpha yeast）培养在含有无机营养盐的营养肉汤中，其中磷酸盐，硝酸盐或钾以25至45℃的温度存在于细胞生长限制浓度中。 pH为4.0至6.0，氧气作为空气和氧气的混合物供应。 根据本发明，在营养液中以0.03-0.15-1的比例，在肉汤中以0.1至1.0％的浓度加入由甘油，山梨糖醇或木糖组成的有机底物，对醇醇氧化酶具有抑制作用。

    Method for conducting fermentation
    23.
    发明授权
    Method for conducting fermentation 失效
    发酵方法

    公开(公告)号：US3957585A

    公开(公告)日：1976-05-18

    申请号：US545546

    申请日：1975-01-30

    申请人： Emil A. Malick

    发明人： Emil A. Malick

    IPC分类号： C12M1/08 ,  C12B1/14

    CPC分类号： C12M27/02 ,  C12M27/24 ,  C12M41/24 ,  Y10S435/804 ,  Y10S435/812 ,  Y10S435/813 ,  Y10S435/818 ,  Y10S435/874 ,  Y10S435/93

    摘要： An apparatus for conducting a fermentation process for the production of single cell protein and the like. The apparatus includes a vessel with a reaction zone and has liquid atomizing means opening thereinto for the injection of atomized liquid into the reaction zone to effect intimate contact of the atomized liquid with a gas phase in the reaction zone. Fermentation medium is supplied to the reaction zone and provides a source of carbon and energy for the culturing of a microorganism and preferably before introduction into the reaction zone said medium is saturated with an oxygen-containing gas.

    摘要翻译： 用于进行用于产生单细胞蛋白质等的发酵方法的装置。 该装置包括具有反应区的容器，并具有用于将雾化液体注入反应区的液体雾化装置，以使雾化液体与反应区中的气相紧密接触。 将发酵培养基供应到反应区，并提供用于培养微生物的碳源和能量源，优选在引入反应区之前，所述培养基用含氧气体饱和。

    Process for cultivating ethanol-assimilating yeasts
    24.
    发明授权
    Process for cultivating ethanol-assimilating yeasts 失效
    培养乙醇同化酵母的方法

    公开(公告)号：US3929578A

    公开(公告)日：1975-12-30

    申请号：US51901974

    申请日：1974-10-29

    申请人： MITSUBISHI GAS CHEMICAL CO

    发明人： URAKAMI TEIZI

    IPC分类号： C12N1/32 ,  C12B1/00

    CPC分类号： C12N1/32 ,  Y10S435/921 ,  Y10S435/93 ,  Y10S435/938 ,  Y10S435/94 ,  Y10S435/942 ,  Y10S435/944

    摘要： Ethanol-assimilating yeasts of, for example, the genus Pichia, Mycoderma, Candida, Saccharomyces, Hansenula, Endomyces, Debaryomyces, Cryptococcus, Torulopsis Rhodotorula or Lipomyces are cultivated with a higher productivity in a higher yield without any contamination of bacteria by conducting cultivation at an ethanol concentration of not more than 0.8% by weight, a dissolved oxygen concentration of 0.1 to 30 ppm, a dissolved carbon dioxide concentration of 0.38 to 380 ppm, pH of 2.0 to 5.0 and temperature of 25* to 48*C. The resulting yeasts can be utilized as food or fodder directly or after treatments, or as a raw material for extraction of valuable substances, nucleic acids, vitamins, coenzymes, proteins, and lipids therefrom.

    摘要翻译： 以更高的产率以更高的生产率培养例如毕赤酵母属，真菌病，假丝酵母​​属，酵母属，酵母菌属，假丝酵母​​属，酵母属，汉逊酵母属，内分泌酵母属，德巴利酵母属，隐球菌属，Torulopsis Rhodotorula或Lipomyces的乙醇 - 同化酵母，而不会通过在 乙醇浓度不超过0.8重量％，溶解氧浓度为0.1〜30ppm，溶解二氧化碳浓度为0.38〜380ppm，pH为2.0〜5.0，温度为25〜48℃。 酵母可以直接或经处理后用作食物或饲料，也可用作从中提取有价值物质，核酸，维生素，辅酶，蛋白质和脂质的原料。

    Production of mannans by fermentation
    26.
    发明授权
    Production of mannans by fermentation 失效
    通过发酵生产芒硝

    公开(公告)号：US3713979A

    公开(公告)日：1973-01-30

    申请号：US3713979D

    申请日：1971-05-28

    申请人： US AGRICULTURE

    发明人： SLODKI M ,  SMILEY M ,  HENSLEY D

    IPC分类号： C12P19/04 ,  C12D1/00

    CPC分类号： C12P19/04 ,  Y10S435/93 ,  Y10S435/938 ,  Y10S435/944

    摘要： Mannan polymers are produced extracellularly by yeast previously known to produce only phosphomannans. Hydrolysis of the polymers with a mineral acid catalyst results in substantially pure Dmannose.

    摘要翻译： 曼南聚合物是由以前已知只能生产磷酸甘露聚糖的酵母细胞外产生的。 用无机酸催化剂水解聚合物导致基本上纯的D-甘露糖。

    Method for producing optically active mandelic acid derivatives
    28.
    发明授权
    Method for producing optically active mandelic acid derivatives 失效
    光学活性扁桃酸衍生物的制备方法

    公开(公告)号：US06777224B2

    公开(公告)日：2004-08-17

    申请号：US10278268

    申请日：2002-10-23

    申请人： Kazuya Mitsuhashi ,  Hiroaki Yamamoto

    发明人： Kazuya Mitsuhashi ,  Hiroaki Yamamoto

    IPC分类号： C07C100

    CPC分类号： C12P7/42 ,  Y10S435/829 ,  Y10S435/83 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/853 ,  Y10S435/859 ,  Y10S435/93 ,  Y10S435/94

    摘要： The present invention provides a method for enzymatically producing optically active mandelic acid derivatives. An optically active mandelic acid derivative (shown as Formula II) is produced by reacting a culture or cell body of a microorganism, or processed products thereof, with a phenylglyoxylic acid derivative, and then recovering the obtained optically active mandelic acid derivative, wherein the microorganism has the ability to stereo-selectively reduce the phenylglyoxylic acid derivative. An optically active mandelic acid obtained according to the present invention is useful as an intermediate for the synthesis of pharmaceuticals and agricultural chemicals.

    摘要翻译： 本发明提供了一种酶促生产光学活性扁桃酸衍生物的方法。 通过使微生物的培养物或细胞体或其加工产物与苯基乙醛酸衍生物反应，然后回收获得的光学活性扁桃酸衍生物，制备光学活性扁桃酸衍生物（如式II所示），其中微生物 具有立体选择性还原苯乙醛酸衍生物的能力。 根据本发明获得的光学活性扁桃酸可用作药物和农药的合成中间体。

    Process for the preparation of chiral 1-aryl-2-aminopropanes
    29.
    发明授权
    Process for the preparation of chiral 1-aryl-2-aminopropanes 失效
    手性1-芳基-2-氨基丙烷的制备方法

    公开(公告)号：US5360724A

    公开(公告)日：1994-11-01

    申请号：US992575

    申请日：1992-12-18

    申请人： George W. Matcham ,  Seujo Lee

    发明人： George W. Matcham ,  Seujo Lee

    IPC分类号： C07C209/26 ,  C07C209/52 ,  C07C209/62 ,  C07C213/02 ,  C12P7/26 ,  C12P41/00 ,  C12P13/00

    CPC分类号： C07C213/02 ,  C07C209/26 ,  C07C209/52 ,  C07C209/62 ,  C12P41/006 ,  C12P7/26 ,  Y10S435/832 ,  Y10S435/874 ,  Y10S435/886 ,  Y10S435/913 ,  Y10S435/921 ,  Y10S435/93 ,  Y10S435/94

    摘要： One chiral form of a 1-aryl-2-aminopropane is produced in preference to its enantiomer by allowing a 1-arylpropan-2-one to react with a 1-amino-1-phenylethane of predominantly one chiral form and reducing the resultant 1-(1-arylprop-2-ylideneimino)-1-phenylethane to yield phenylethane and a mixture of 1-aryl-2-aminopropanes in which one chiral form thereof is present in preference to its enantiomer. The mixture of 1-aryl-2-aminopropanes then is subjected to the action of an omega-amino acid transaminase which converts one of the two chiral forms of 1-aryl-2-aminopropane into the corresponding arylpropanone which can be separated from the remaining chiral form of the 1-aryl-2-aminopropane.

    摘要翻译： 通过使1-芳基丙-2-酮与主要具有一个手性形式的1-氨基-1-苯基乙烷反应，并且将所得的1 - 芳基-2-氨基丙烷与其对映异构体1反应，生成1-芳基-2-氨基丙烷的一种手性形式 - （1-芳基丙-2-亚基亚氨基）-1-苯基乙烷，得到苯乙烷和1-芳基-2-氨基丙烷的混合物，其中优选其对映异构体存在一种手性形式。 然后将1-芳基-2-氨基丙烷的混合物进行ω-氨基酸转氨酶的作用，其将两个手性形式的1-芳基-2-氨基丙烷转化成相应的芳基丙酮，其可以与剩余的 手性形式的1-芳基-2-氨基丙烷。

    Nitrate reductase from yeasts, the preparation and use thereof
    30.
    发明授权
    Nitrate reductase from yeasts, the preparation and use thereof 失效
    来自酵母的硝酸还原酶，其制备和使用

    公开(公告)号：US5294539A

    公开(公告)日：1994-03-15

    申请号：US781165

    申请日：1991-10-16

    申请人： Walther Johannssen ,  Harry Schwartz ,  Reiner Gromes ,  Martin Heinrich

    发明人： Walther Johannssen ,  Harry Schwartz ,  Reiner Gromes ,  Martin Heinrich

    IPC分类号： C12N9/02 ,  C12N9/96 ,  C12Q1/26 ,  C12R1/72 ,  C12Q1/12

    CPC分类号： C12N9/0036 ,  C12N9/96 ,  C12Q1/26 ,  C12Y107/01002 ,  Y10S435/814 ,  Y10S435/921 ,  Y10S435/93

    摘要： The invention relates to NAD(P)H-dependent nitrate reductase from yeasts, to a process for the preparation thereof and to the use thereof in a reagent for determining nitrate. The nitrate reductase is characterized by a molecular weight of about 350 000 D and can be obtained by yeast cells which have been cultivated in a completely synthetic nutrient medium with nitrate as the sole nitrogen source and which contain nitrate reductase being disrupted in phosphate buffer, the crude extract being chromatographed on an anion exchanger, the fractions containing nitrate reductase being mixed with protein, concentrated by ultrafiltration and dried by fluidized bed granulation. The reagent for determining nitrate contains, besides the nitrate reductase prepared in this way, also NAD(P)H and a color reagent for determining nitrite.

    摘要翻译： PCT No.PCT / EP91 / 00199 Sec。 371日期1991年10月16日 102（e）日期1991年10月16日PCT 1991年2月2日PCT PCT。 公开号WO91 / 12319 日本1991年8月22日。本发明涉及来自酵母的NAD（P）H依赖性硝酸还原酶，涉及其制备方法及其在用于测定硝酸盐的试剂中的用途。 硝酸还原酶的特征在于分子量约为350000D，可以通过在硝酸盐作为唯一氮源并且含有硝酸还原酶在磷酸盐缓冲液中被破坏的完全合成营养培养基中培养的酵母细胞得到， 粗提物在阴离子交换剂上进行色谱分离，含有硝酸还原酶的级分与蛋白质混合，通过超滤浓缩并通过流化床造粒干燥。 用于测定硝酸盐的试剂除了以这种方式制备的硝酸盐还原酶之外，还含有NAD（P）H和用于测定亚硝酸盐的着色剂。