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1652 条记录 (耗时 0.035 秒)

    Novel fructosyltransferases
    21.
    发明申请
    Novel fructosyltransferases 审中-公开
    新型果糖基转移酶

    公开(公告)号：US20040185537A1

    公开(公告)日：2004-09-23

    申请号：US10791799

    申请日：2004-03-04

    申请人： NEDERLANDSE ORGANISATIE VOOR TOEGEPAST NATUURWETENSCHAPPELIJK ONDERZOEK TNO

    发明人： Sacha Adrianus Fokke Taco Van Hijum ,  Gerritdina Hendrika Van Geel-Schutten ,  Lubbert Dijkhuizen ,  Hakim Rahaoui

    IPC分类号： C12P019/04 ,  C12N009/10 ,  C12N001/21

    CPC分类号： C12N9/1051 ,  A61K35/747 ,  C12N9/1055

    摘要： The present invention describes two novel proteins having fructosyltransferase activity. Both enzymes are derived from lactobacilli, which are food-grade micro-organisms with the Generally Recognized As Safe (GRAS) status. One of these proteins produces an inulin and fructo-oligosaccharides, while the other produces a levan and fructo-oligosaccharides. According to the invention lactobacilli capable of producing an inulin and/or a levan and/or fructo-oligosaccharides using one or both of the fructosyltransferases can be used as a probiotic or a symbiotic.

    摘要翻译： 本发明描述了两种具有果糖基转移酶活性的新型蛋白质。 这两种酶均来自乳酸杆菌，其是具有“一般认可安全”（GRAS）状态的食品级微生物。 这些蛋白质中的一种产生菊粉和果寡糖，而另一种则产生果糖和低聚果糖。 根据本发明，使用一个或两个果糖基转移酶能够产生菊粉和/或levan和/或果寡糖的乳杆菌可以用作益生菌或共生体。

    Recombinant proteins containing Shiga-like toxin and vascular endothelial growth factor fragments
    22.
    发明申请
    Recombinant proteins containing Shiga-like toxin and vascular endothelial growth factor fragments 有权
    含有志贺样毒素和血管内皮生长因子片段的重组蛋白

    公开(公告)号：US20040166565A1

    公开(公告)日：2004-08-26

    申请号：US10765580

    申请日：2004-01-27

    发明人： Marina V. Backer ,  Joseph M. Backer

    IPC分类号： A61K038/18 ,  C07K014/475 ,  C12N001/21 ,  C07H021/04

    CPC分类号： C07K14/245 ,  A61K38/00 ,  C07K14/52 ,  C07K2319/00

    摘要： The present invention is directed to an isolated nucleic acid encoding a fusion protein comprising (1) the A subunit of Shiga-like bacterial toxin, or a truncated or mutated version thereof; and (2) human vascular endothelial growth factor, or a truncated or mutated version thereof; wherein the fusion protein possesses ribosome inactivating activity and ability to bind to cellular VEGF receptors. The present invention is also directed to polypeptides the above combination of toxin and growth factor, as well as expression vectors and transformed cells incorporating the above nucleic acid. The invention is also directed to pharmaceutical compositions and methods for treating patients suffering from diseases relating to angiogenesis.

    摘要翻译： 本发明涉及编码融合蛋白的分离核酸，其包含（1）志贺样细菌毒素的A亚基或其截短或突变型; 和（2）人血管内皮生长因子，或其截短或突变型; 其中所述融合蛋白具有结合细胞VEGF受体的核糖体失活活性和能力。 本发明还涉及上述毒素和生长因子的组合以及包含上述核酸的表达载体和转化细胞的多肽。 本发明还涉及用于治疗患有与血管生成有关的疾病的患者的药物组合物和方法。

    Alcohol dehydrogenases with increased solvent and temperature stability
    24.
    发明申请
    Alcohol dehydrogenases with increased solvent and temperature stability 有权
    醇脱氢酶具有增加的溶剂和温度稳定性

    公开(公告)号：US20040157305A1

    公开(公告)日：2004-08-12

    申请号：US10666205

    申请日：2003-09-18

    发明人： Wolfgang Stampfer ,  Birgit Kosjek ,  Wolfgang Kroutil ,  Kurt Faber ,  Jurgen Eck ,  Frank Niehaus

    IPC分类号： C12N001/21 ,  C12N009/02

    CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P41/002

    摘要： The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and/or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and/or reducing ketones.

    摘要翻译： 本发明涉及显示可从红球菌获得的醇脱氢酶活性的生物催化剂，它们的制备，它们在仲醇氧化中的用途和/或还原酮，以及编码这些醇脱氢酶的核酸和用编码的核酸转化的微生物 这些生物催化剂及其用于生产生物催化剂或氧化仲醇和/或还原酮的用途。

    Pet family of efflux proteins
    26.
    发明申请
    Pet family of efflux proteins 失效
    宠物家庭的外排蛋白

    公开(公告)号：US20040147000A1

    公开(公告)日：2004-07-29

    申请号：US10759898

    申请日：2004-01-16

    发明人： Fateme Sima Sariaslani ,  Tina K. Van Dyk

    IPC分类号： C12P007/40 ,  C12N001/21 ,  C12N001/18 ,  A01H001/00

    CPC分类号： C12P7/40 ,  C07K14/245

    摘要： The invention provides methods of increasing the production of aromatic carboxylic acids from a host cell via manipulation of the yhcRQP operon encoding a family of efflux proteins. Up-regulation of all or a sub-set of the genes in the yhcRQP were additionally found to enhance tolerance to aromatic carboxylic acids toxicity.

    摘要翻译： 本发明提供了通过操纵编码一系列外排蛋白的yhcRQP操纵子来增加宿主细胞产生芳族羧酸的方法。 另外发现yhcRQP中全部或一组亚基的上调增强了对芳香族羧酸毒性的耐受性。

    Enhanced production of Streptococcus mutans mutacin I and III
    27.
    发明申请
    Enhanced production of Streptococcus mutans mutacin I and III 审中-公开
    增强变形链球菌Mutacin I和III的生产

    公开(公告)号：US20040146983A1

    公开(公告)日：2004-07-29

    申请号：US10672784

    申请日：2003-09-26

    发明人： Page W. Caufield ,  Zengguo He

    IPC分类号： C12P021/02 ,  C12N001/21

    CPC分类号： C12P21/02 ,  C07K14/315

    摘要： Methods for producing a mutacin, by growing a mutacin-producing cell in a liquid medium under conditions in which mutacin is produced and isolating mutacin from the liquid medium. Methods are also provided for isolating and purifying mutacin I/III from fermentation broths of Streptococcus mutans strains CH43 and UA787 to homogeneity. Fermentation are conducted in anaerobic bioreactors under anaerobic conditions at a cultivation temperature of about 35-42null C., with agitation rate of between 50-250 rpm.

    摘要翻译： 通过在液体培养基中生产Mutacin产生细胞，在产生Mutacin的条件下生成Mutacin并从液体培养基中分离Mutacin的方法。 还提供了用于从变形链球菌菌株CH43和UA787的发酵液中分离和纯化Mutacin I / III的方法，使其具有均匀性。 在厌氧条件下，在约35-42℃的培养温度下，在50-250rpm的搅拌速度下，在厌氧生物反应器中进行发酵。

    Recombinant chalcomycin polyketide synthase and modifying genes
    29.
    发明申请
    Recombinant chalcomycin polyketide synthase and modifying genes 审中-公开
    重组chalcomycin聚酮化合物合成酶和修饰基因

    公开(公告)号：US20040132055A1

    公开(公告)日：2004-07-08

    申请号：US10647196

    申请日：2003-08-21

    发明人： Leonard Katz ,  Ralph C. Reid ,  Zhihao Hu ,  Andreas Schirmer ,  Shannon L. Ward ,  Christopher Reeves

    IPC分类号： C12Q001/68 ,  C12P019/60 ,  C07H021/04 ,  C12N009/10 ,  C12N001/21

    CPC分类号： C12N15/52

    摘要： Domains of chalcomycin polyketide synthases and modification enzymes and polynucleotides encoding them are provided. Methods to prepare chalcomycin in pharmaceutically useful quantities are described, as are methods to prepare chalcomycin analogs and other polyketides using the polynucleotides encoding chalcomycin polyketide synthase domains or modifying enzymes.

    摘要翻译： 提供了霉菌素聚酮化合物合酶及其修饰酶和编码它们的多核苷酸的结构域。 描述了以药学上可用的量制备沙门霉素的方法，以及使用编码chalcomycin聚酮合成酶结构域或修饰酶的多核苷酸制备沙门霉素类似物和其它聚酮化合物的方法。