公开(公告)号：US11441130B2

公开(公告)日：2022-09-13

申请号：US15319272

申请日：2015-06-16

Applicant: GENOMATICA, INC.

Inventor： Baolong Zhu ,  Andreas W. Schirmer ,  Cindy Chang

IPC: C12N9/02 ,  C12P7/6409

Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

公开(公告)号：US11441128B2

公开(公告)日：2022-09-13

申请号：US15771973

申请日：2016-10-27

Applicant: Genomatica, Inc.

Inventor： Nelson R. Barton ,  Jingyi Li ,  Joseph R. Warner ,  Priti Pharkya

IPC: C12N9/00 ,  C12N9/10 ,  C12N9/90 ,  C12N9/88 ,  C12N15/52 ,  C12N9/04 ,  C12P7/24

Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US20220267813A1

公开(公告)日：2022-08-25

申请号：US17732799

申请日：2022-04-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Noah HELMAN ,  Haibo WANG ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Alma Itzel RAMOS-SOLIS ,  Elizabeth CLARKE

IPC: C12P7/18 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52

Abstract: The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

公开(公告)号：US20220243185A1

公开(公告)日：2022-08-04

申请号：US17715587

申请日：2022-04-07

Applicant: Genomatica, Inc.

Inventor： Angelica Zabala BAUTISTA ,  Min Sun PARK ,  Alma Itzel RAMOS-SOLIS ,  Bernardo Moura TORRES DA COSTA ,  Behnaz BEHROUZIAN ,  Donald E. TRIMBUR ,  Emanuela POPOVA ,  Qijun HU ,  Stephen Bralley DEL CARDAYRE

IPC: C12N9/16 ,  C12P7/6409

Abstract: The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.

公开(公告)号：US20220220511A1

公开(公告)日：2022-07-14

申请号：US17394184

申请日：2021-08-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12P7/16 ,  C12N15/52

Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.

公开(公告)号：US20220177895A1

公开(公告)日：2022-06-09

申请号：US17255352

申请日：2019-06-26

Applicant: Genomatica, Inc.

Inventor： Harish Nagarajan ,  Tae Hoon Yang ,  Ali Khodayari

IPC: C12N15/52 ,  C12N9/02 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12P13/00 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P7/46 ,  C12P7/6409

Abstract: Described herein are engineered cells including ones having synthetic methylotrophy which include an NADH-dependent enzyme capable of converting G3P to 3PG (e.g., B. methanolicus gapN) and/or fructose-1,6-bisphosphatase, along with hexulose-6-phosphate synthase, 6-phospho-3-hexuloisomerase, a phosphoketolase, or a combination thereof. Engineered cells of the disclosure beneficially maintain adequate pool sizes of phosphorylated C3 and/or C4 compounds, and/or provide increased levels of NADPH. As such, the modifications allow for the generation of C6 compounds from C1 (e.g., a methanol feedstod) and C5 compounds, the regeneration of C5 compounds from C6 compounds by carbon rearrangement, and an improved balance between regeneration of C5 compounds and lower glycolysis. In turn, this allows the engineered microorganism to generate sufficient quantities of metabolic precursors (e.g., acetyl-CoA) which can be used in a bioproduct pathway, and the engineered cells can include further modifications to those pathway enzymes allowing for production of a desired bioproduct.

公开(公告)号：US11299716B2

公开(公告)日：2022-04-12

申请号：US16499531

申请日：2018-03-29

Applicant: Genomatica, Inc.

Inventor： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Amit Shah ,  Joseph Warner ,  Michael Noble

IPC: C12N9/02

Abstract: The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

公开(公告)号：US20220056488A1

公开(公告)日：2022-02-24

申请号：US17225034

申请日：2021-04-07

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

IPC: C12P7/62 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00

Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

公开(公告)号：US20220025411A1

公开(公告)日：2022-01-27

申请号：US17193914

申请日：2021-03-05

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P5/02 ,  C12N15/52 ,  C07C11/16 ,  C08F136/06

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US20210371889A1

公开(公告)日：2021-12-02

申请号：US17350690

申请日：2021-06-17

Applicant: Genomatica, Inc.

Inventor： Derek L. GREENFIELD ,  Louis G. HOM ,  Fernando A. SANCHEZ-RIERA ,  Zhihao HU ,  Vikranth ARLAGADDA ,  Eli S. GROBAN ,  Scott A. FRYKMAN

IPC: C12P7/64 ,  C07K14/245 ,  C07K14/195 ,  C07C31/125 ,  C07C33/02 ,  C07C53/126

Abstract: The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.