公开(公告)号：US09982281B2

公开(公告)日：2018-05-29

申请号：US14692630

申请日：2015-04-21

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12P7/18 ,  C12N15/52

CPC classification number: C12P7/18 ,  C12N15/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：US20180100172A1

公开(公告)日：2018-04-12

申请号：US15599314

申请日：2017-05-18

Applicant: Genomatica, Inc.

Inventor： Petronella Catharina RAEMAKERS-FRANKEN ,  Martin SCHURMANN ,  Axel Christoph TREFZER ,  Stefaan Marie Andre DE WILDEMAN

IPC: C12P13/00 ,  C12P13/02 ,  C07C227/06 ,  C07D201/08 ,  C07D223/10 ,  C07C229/08

CPC classification number: C12P13/001 ,  C07C227/06 ,  C07D201/08 ,  C07D223/10 ,  C12P13/005 ,  C12P13/02 ,  C07C229/08

Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing ϵ-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.

公开(公告)号：US09909150B2

公开(公告)日：2018-03-06

申请号：US14071318

申请日：2013-11-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14 ,  C12P7/20 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52

CPC classification number: C12P7/24 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/20 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.

公开(公告)号：US09885064B2

公开(公告)日：2018-02-06

申请号：US14185709

申请日：2014-02-20

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

IPC: C12N1/20 ,  C12N1/12 ,  C12P7/62 ,  C12P7/04 ,  C07H21/04 ,  C12P7/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12P19/32 ,  C12N15/70

CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

公开(公告)号：US09732361B2

公开(公告)日：2017-08-15

申请号：US14059131

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C08F36/00 ,  C12P5/02 ,  C12N1/20 ,  C12N1/00 ,  C07C9/00 ,  C12N15/52 ,  C07C11/16 ,  C08F136/06

CPC classification number: C12P5/026 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US20170183694A1

公开(公告)日：2017-06-29

申请号：US15191421

申请日：2016-06-23

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCHINSKAS ,  Brian STEER ,  Stefan ANDRAE ,  Amit SHAH

IPC: C12P7/18 ,  C12P7/42 ,  C12N1/20 ,  C12P17/04 ,  C07C211/09 ,  C12P13/00 ,  C07C47/19 ,  C07C31/20 ,  C07D307/33 ,  C12N15/52 ,  C12N9/10

CPC classification number: C12P7/18 ,  C07C31/207 ,  C07C47/19 ,  C07C211/09 ,  C07D307/33 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/13 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P17/04 ,  C12P19/32 ,  C12Y208/03

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

公开(公告)号：US09663805B2

公开(公告)日：2017-05-30

申请号：US14105705

申请日：2013-12-13

Applicant: Genomatica, Inc

Inventor： Petronella Catharina Raemakers-Franken ,  Martin Schurmann ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman

IPC: C12P13/02 ,  C12P7/40 ,  C12P7/26 ,  C12P13/00 ,  C07C227/06 ,  C07D223/10 ,  C07D201/08

CPC classification number: C12P13/001 ,  C07C227/06 ,  C07D201/08 ,  C07D223/10 ,  C12P13/005 ,  C12P13/02 ,  C07C229/08

Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.

公开(公告)号：US20170073691A1

公开(公告)日：2017-03-16

申请号：US15133150

申请日：2016-04-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Cara Ann TRACEWELL ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12N15/52 ,  C12N9/10 ,  C12N9/06 ,  C12P7/62 ,  C12N9/04

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。

公开(公告)号：US20170022508A1

公开(公告)日：2017-01-26

申请号：US15141002

申请日：2016-04-28

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya

IPC: C12N15/52 ,  C12P13/00 ,  C12N9/88 ,  C12N15/70 ,  C12N9/10

CPC classification number: C12N15/52 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/70 ,  C12P13/001 ,  C12Y206/01085 ,  C12Y401/01024 ,  C12Y401/03038

Abstract: A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline.

Abstract translation: 具有苯胺途径的非天然存在的微生物包括至少一种编码以足够量表达以产生苯胺的苯胺途径酶的外源核酸。 苯胺途径包括（1）氨基脱氧胸苷酸合酶，氨基脱氧胸苷酸裂合酶和4-氨基苯甲酸羧基酯酶或（2）邻氨基苯甲酸合酶和邻氨基苯甲酸脱羧酶。 制备苯胺的方法包括在条件和足够的时间内培养这些非天然存在的微生物生物产生苯胺。

公开(公告)号：US20160355846A1

公开(公告)日：2016-12-08

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Robert HASELBECK ,  Catherine J. PUJOL-BAXLEY ,  Wei NIU ,  John D. TRAWICK ,  Harry YIM ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Jun SUN

IPC: C12P7/18 ,  C12N9/10 ,  C12N15/52 ,  C12N9/16 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Abstract translation: 本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化用于BDO的表达。 本发明还提供了使用这种微生物生产BDO的方法。